{"title":"Fast Restriction Endonuclease","description":"\u003cp\u003e\u003cspan\u003eAll GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.\u003c\/span\u003e\u003c\/p\u003e","products":[{"product_id":"bglii","title":"BglII","description":"\u003cdiv\u003e\u003cspan\u003e\u003cspan data-spm-anchor-id=\"a2700.details.0.i67.5c5e27cetDLebi\"\u003eRecognition site:\u003cbr\u003e5' A ↓ G A T C T 3' \u003cbr\u003e3' T C C A G ↑ A 5' \u003cbr\u003e\u003cbr\u003eProduct Description \u003cbr\u003eThe BglⅡ restriction endonuclease recognizes the A^GATCT site and achieves optimal cutting efficiency within 5–15 minutes at 37°C using the universal buffer.\u003cbr\u003eGDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.\u003cbr\u003eThe universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.\u003cbr\u003eThe Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.\u003c\/span\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003eStorage Condition\u003cbr\u003eStore at -20°C.\u003cbr\u003e\u003cbr\u003eFeatures\u003cbr\u003e- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.\u003cbr\u003e- 100% buffer compatibility with downstream applications.\u003cbr\u003e- Enzymatic digestion can be completed within 5–15 minutes.\u003cbr\u003e- Direct loading onto a gel.\u003cbr\u003e- No star activity.\u003cbr\u003e\u003cbr\u003eScope of Application \u003cbr\u003e- Molecular cloning\u003cbr\u003e- Restriction site mapping\u003cbr\u003e- Genotyping\u003cbr\u003e- Southern blotting\u003cbr\u003e- Restriction fragment length polymorphism (RFLP)\u003cbr\u003e- SNP (Single Nucleotide Polymorphism) analysis\u003c\/span\u003e\u003c\/div\u003e","brand":"GDSBIO","offers":[{"title":"100 rxns","offer_id":41528664916102,"sku":"E1023-A","price":60.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_0a7b6e01-93a4-4d22-a169-477dcadfa18a.jpg?v=1737093492"},{"product_id":"ecori","title":"EcoRI","description":"\u003cdiv\u003e\u003cspan\u003e\u003cspan data-spm-anchor-id=\"a2700.details.0.i67.710f5de0sOSYKF\"\u003eRecognition site:\u003cbr\u003e5' G ↓ A A T T C 3' \u003cbr\u003e3' C T T A A ↑ G 5' \u003cbr\u003e\u003cbr\u003eProduct Description \u003cbr\u003eThe EcoRⅠ restriction endonuclease recognizes the G^AATTC site and achieves optimal cutting efficiency within 5–15 minutes at 37°C using the universal buffer.\u003c\/span\u003e\u003cbr\u003eGDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.\u003cbr\u003eThe universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.\u003cbr\u003eThe Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003eStorage Condition \u003cbr\u003eStore at -20°C.\u003cbr\u003e\u003cbr\u003eFeatures\u003cbr\u003e- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.\u003cbr\u003e- 100% buffer compatibility with downstream applications.\u003cbr\u003e- Enzymatic digestion can be completed within 5–15 minutes.\u003cbr\u003e- Direct loading onto a gel.\u003cbr\u003e- No star activity.\u003cbr\u003e\u003cbr\u003eScope of Application \u003cbr\u003e- Molecular cloning\u003cbr\u003e- Restriction site mapping\u003cbr\u003e- Genotyping\u003cbr\u003e- Southern blotting\u003cbr\u003e- Restriction fragment length polymorphism (RFLP)\u003cbr\u003e- SNP (Single Nucleotide Polymorphism) analysis\u003c\/span\u003e\u003c\/div\u003e","brand":"GDSBIO","offers":[{"title":"800 rxns","offer_id":41528664948870,"sku":"E1024-A","price":62.0,"currency_code":"USD","in_stock":true},{"title":"2,500 rxns","offer_id":41528664981638,"sku":"E1024-B","price":174.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_56c3fe0f-3c3b-46f4-9d1c-ded2e88efa3e.jpg?v=1737093496"},{"product_id":"hindiii","title":"HindIII","description":"\u003cdiv data-spm-anchor-id=\"a2700.details.0.i68.73c136b5XDk4WU\"\u003e\u003cspan\u003e\u003cspan data-spm-anchor-id=\"a2700.details.0.i69.73c136b5XDk4WU\"\u003eRecognition site:\u003cbr\u003e5' A ↓ A G C T T 3' \u003cbr\u003e3' T T C G A ↑ A 5' \u003cbr\u003e\u003cbr\u003eProduct Description \u003cbr\u003eThe HindⅢ restriction endonuclease recognizes the A^AGCTT site and achieves optimal cutting efficiency within 5–15 minutes at 37°C using the universal buffer.\u003c\/span\u003e\u003cbr\u003eGDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.\u003cbr\u003eThe universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.\u003cbr\u003eThe Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003eStorage Condition \u003cbr\u003eStore at -20°C.\u003cbr\u003e\u003cbr\u003eFeatures\u003cbr\u003e- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.\u003cbr\u003e- 100% buffer compatibility with downstream applications.\u003cbr\u003e- Enzymatic digestion can be completed within 5–15 minutes.\u003cbr\u003e- Direct loading onto a gel.\u003cbr\u003e- No star activity.\u003c\/span\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cbr\u003eScope of Application \u003cbr\u003e- Molecular cloning\u003cbr\u003e- Restriction site mapping\u003cbr\u003e- Genotyping\u003cbr\u003e- Southern blotting\u003cbr\u003e- Restriction fragment length polymorphism (RFLP)\u003cbr\u003e- SNP (Single Nucleotide Polymorphism) analysis\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"E1025-A\/800 rxns","offer_id":41528665079942,"sku":"E1025-A","price":72.0,"currency_code":"USD","in_stock":true},{"title":"E1025-B\/2,500 rxns","offer_id":41528665112710,"sku":"E1025-B","price":226.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_36e10216-78fe-467e-af32-b830d7d4980c.jpg?v=1737093500"},{"product_id":"ncoi","title":"NcoI","description":"\u003cdiv\u003e\u003cspan\u003e\u003cspan data-spm-anchor-id=\"a2700.details.0.i68.7f804c2acd43BL\"\u003eRecognition site:\u003cbr\u003e5' C ↓ C A T G G 3' \u003cbr\u003e3' G G T A C ↑ C 5' \u003cbr\u003e\u003cbr\u003eProduct Description \u003cbr\u003eThe NcoⅠ restriction endonuclease recognizes the C^CATGG site and achieves optimal cutting efficiency within 5–15 minutes at 37°C using the universal buffer.\u003c\/span\u003e\u003cbr\u003eGDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.\u003cbr\u003eThe universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.\u003cbr\u003eThe Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003eStorage Condition \u003cbr\u003eStore at -20°C.\u003cbr\u003e\u003cbr\u003eFeatures\u003cbr\u003e- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.\u003cbr\u003e- 100% buffer compatibility with downstream applications.\u003cbr\u003e- Enzymatic digestion can be completed within 5–15 minutes.\u003cbr\u003e- Direct loading onto a gel.\u003cbr\u003e- No star activity.\u003cbr\u003e\u003cbr\u003eScope of Application \u003cbr\u003e- Molecular cloning\u003cbr\u003e- Restriction site mapping\u003cbr\u003e- Genotyping\u003cbr\u003e- Southern blotting\u003cbr\u003e- Restriction fragment length polymorphism (RFLP)\u003cbr\u003e- SNP (Single Nucleotide Polymorphism) analysis\u003c\/span\u003e\u003c\/div\u003e","brand":"GDSBIO","offers":[{"title":"20 rxns","offer_id":41528665178246,"sku":"E1026-A","price":75.0,"currency_code":"USD","in_stock":true},{"title":"100 rxns","offer_id":41528665211014,"sku":"E1026-B","price":117.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_9bcc4d2f-d8b7-4f5e-bdbe-d06d19ac6ce2.jpg?v=1737093504"},{"product_id":"noti","title":"NotI","description":"\u003cdiv\u003e\u003cspan\u003e\u003cspan data-spm-anchor-id=\"a2700.details.0.i67.3faa24aenBO2Pn\"\u003eRecognition site:\u003cbr\u003e5' G C ↓ G G C C G C 3' \u003cbr\u003e3' C G C C G G ↑ C G 5' \u003cbr\u003e\u003cbr\u003eProduct Description \u003cbr\u003eThe NotⅠ restriction endonuclease recognizes the GC^GGCCGC site and achieves optimal cutting efficiency within 5–15 minutes at 37°C using the universal buffer. Isoschizomer: CciNⅠ.\u003c\/span\u003e\u003cbr\u003eGDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.\u003cbr\u003eThe universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.\u003cbr\u003eThe Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003eStorage Condition \u0026amp; Shelf Life\u003cbr\u003eStore at -20°C.\u003cbr\u003e\u003cbr\u003eFeatures\u003cbr\u003e- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.\u003cbr\u003e- 100% buffer compatibility with downstream applications.\u003cbr\u003e- Enzymatic digestion can be completed within 5–15 minutes.\u003cbr\u003e- Direct loading onto a gel.\u003cbr\u003e- No star activity.\u003cbr\u003e\u003cbr\u003eScope of Application \u003cbr\u003e- Molecular cloning\u003cbr\u003e- Restriction site mapping\u003cbr\u003e- Genotyping\u003cbr\u003e- Southern blotting\u003cbr\u003e- Restriction fragment length polymorphism (RFLP)\u003cbr\u003e- SNP (Single Nucleotide Polymorphism) analysis\u003c\/span\u003e\u003c\/div\u003e","brand":"GDSBIO","offers":[{"title":"20 rxns","offer_id":41528665309318,"sku":"E1027-A","price":74.0,"currency_code":"USD","in_stock":true},{"title":"50 rxns","offer_id":41528665342086,"sku":"E1027-B","price":122.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_c375723b-3b7c-4618-9ecd-60c305345b26.jpg?v=1737093509"},{"product_id":"pvui","title":"PvuI","description":"\u003cdiv\u003e\u003cspan\u003e\u003cspan data-spm-anchor-id=\"a2700.details.0.i67.719157e7zwMcMn\"\u003eRecognition site:\u003cbr\u003e5' C G A T ↓ C G 3' \u003cbr\u003e3' G C ↑ T A G C 5' \u003cbr\u003e\u003cbr\u003eProduct Description \u003cbr\u003eThe PvuⅠ restriction endonuclease recognizes the CGAT^CG site and achieves optimal cutting efficiency within 5–15 minutes at 37°C using the universal buffer. Isoschizomers: BpvUⅠ, MvrⅠ, Ple19Ⅰ.\u003c\/span\u003e\u003cbr\u003eGDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.\u003cbr\u003eThe universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.\u003cbr\u003eThe Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003eStorage Condition \u003cbr\u003eStore at -20°C.\u003cbr\u003e\u003cbr\u003eFeatures\u003cbr\u003e- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.\u003cbr\u003e- 100% buffer compatibility with downstream applications.\u003cbr\u003e- Enzymatic digestion can be completed within 5–15 minutes.\u003cbr\u003e- Direct loading onto a gel.\u003cbr\u003e- No star activity.\u003cbr\u003e\u003cbr\u003eScope of Application \u003cbr\u003e- Molecular cloning\u003cbr\u003e- Restriction site mapping\u003cbr\u003e- Genotyping\u003cbr\u003e- Southern blotting\u003cbr\u003e- Restriction fragment length polymorphism (RFLP)\u003cbr\u003e- SNP (Single Nucleotide Polymorphism) analysis\u003c\/span\u003e\u003c\/div\u003e","brand":"GDSBIO","offers":[{"title":"20 rxns","offer_id":41528665374854,"sku":"E1028-A","price":86.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_baa63fa0-5125-421e-8831-6814bd4b3e64.jpg?v=1737093512"},{"product_id":"xhoi","title":"XhoI","description":"\u003cdiv\u003e\u003cspan\u003e\u003cspan data-spm-anchor-id=\"a2700.details.0.i66.15736558IrwwRm\"\u003eRecognition site:\u003cbr\u003e5' C ↓ T C G A G 3' \u003cbr\u003e3' G A G C T ↑ C 5' \u003cbr\u003e\u003cbr\u003eProduct Description \u003cbr\u003eThe XhoⅠ restriction endonuclease recognizes the C^TCGAG site and achieves optimal cutting efficiency within 5–15 minutes at 37°Cusing the universal buffer. Isoschizomers: PaeR7Ⅰ, Sfr274Ⅰ, SlaⅠ, StrⅠ, TliⅠ.\u003c\/span\u003e\u003cbr\u003eGDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.\u003cbr\u003eThe universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.\u003cbr\u003eThe Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003eStorage Condition \u003cbr\u003eStore at -20°C.\u003cbr\u003e\u003cbr\u003eFeatures\u003cbr\u003e- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.\u003cbr\u003e- 100% buffer compatibility with downstream applications.\u003cbr\u003e- Enzymatic digestion can be completed within 5–15 minutes.\u003cbr\u003e- Direct loading onto a gel.\u003cbr\u003e- No star activity.\u003cbr\u003e\u003cbr\u003eScope of Application \u003cbr\u003e- Molecular cloning\u003cbr\u003e- Restriction site mapping\u003cbr\u003e- Genotyping\u003cbr\u003e- Southern blotting\u003cbr\u003e- Restriction fragment length polymorphism (RFLP)\u003cbr\u003e- SNP (Single Nucleotide Polymorphism) analysis\u003c\/span\u003e\u003c\/div\u003e","brand":"GDSBIO","offers":[{"title":"400 rxns","offer_id":41528665407622,"sku":"E1029-A","price":59.0,"currency_code":"USD","in_stock":true},{"title":"1,200 rxns","offer_id":41528665440390,"sku":"E1029-B","price":169.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_e80fdbb7-e21d-48c6-abec-7db6f7549acc.jpg?v=1737093515"},{"product_id":"nhei","title":"NheI","description":"\u003cdiv\u003e\u003cspan\u003e\u003cspan data-spm-anchor-id=\"a2700.details.0.i66.6db0314ez3CCZU\"\u003eRecognition site:\u003cbr\u003e5' G ↓ C T A G C 3' \u003cbr\u003e3' C G A T C ↑ G 5' \u003cbr\u003e\u003cbr\u003eProduct Description \u003cbr\u003eThe NheⅠ restriction endonuclease recognizes the G^CTAGC site and achieves optimal cutting efficiency within 5–15 minutes at 37°Cusing the universal buffer. Isoschizomers: AsuNHⅠ, BmtⅠ.\u003c\/span\u003e\u003cbr\u003eGDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.\u003cbr\u003eThe universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.\u003cbr\u003eThe Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003eStorage Condition \u003cbr\u003eStore at -20°C.\u003cbr\u003e\u003cbr\u003eFeatures\u003cbr\u003e- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.\u003cbr\u003e- 100% buffer compatibility with downstream applications.\u003cbr\u003e- Enzymatic digestion can be completed within 5–15 minutes.\u003cbr\u003e- Direct loading onto a gel.\u003cbr\u003e- No star activity.\u003cbr\u003e\u003cbr\u003eScope of Application \u003cbr\u003e- Molecular cloning\u003cbr\u003e- Restriction site mapping\u003cbr\u003e- Genotyping\u003cbr\u003e- Southern blotting\u003cbr\u003e- Restriction fragment length polymorphism (RFLP)\u003cbr\u003e- SNP (Single Nucleotide Polymorphism) analysis\u003c\/span\u003e\u003c\/div\u003e","brand":"GDSBIO","offers":[{"title":"50 rxns","offer_id":41528665505926,"sku":"E1030-A","price":75.0,"currency_code":"USD","in_stock":true},{"title":"100 rxns","offer_id":41528665538694,"sku":"E1030-B","price":133.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_038e1695-8019-4df1-a75e-c96bd0b98d71.jpg?v=1737093518"},{"product_id":"bamhi","title":"BamHI","description":"\u003cdiv\u003e\u003cspan\u003e\u003cspan data-spm-anchor-id=\"a2700.details.0.i65.6ba1665bX8sWMD\"\u003eRecognition site:\u003cbr\u003e5' G ↓ G A T C C 3' \u003cbr\u003e3' C C T A G ↑ G 5' \u003cbr\u003e\u003cbr\u003eProduct Description \u003cbr\u003eThe BamHⅠ restriction endonuclease recognizes the G^GATCC site and achieves optimal cutting efficiency within 5–15 minutes at 37°C using the universal buffer.\u003c\/span\u003e\u003cbr\u003eGDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.\u003cbr\u003eThe universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.\u003cbr\u003eThe Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003eStorage Condition \u003cbr\u003eStore at -20°C.\u003cbr\u003e\u003cbr\u003eFeatures\u003cbr\u003e- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.\u003cbr\u003e- 100% buffer compatibility with downstream applications.\u003cbr\u003e- Enzymatic digestion can be completed within 5–15 minutes.\u003cbr\u003e- Direct loading onto a gel.\u003cbr\u003e- No star activity.\u003c\/span\u003e\u003c\/div\u003e\n\u003cp\u003e\u003cbr\u003eScope of Application \u003cbr\u003e- Molecular cloning\u003cbr\u003e- Restriction site mapping\u003cbr\u003e- Genotyping\u003cbr\u003e- Southern blotting\u003cbr\u003e- Restriction fragment length polymorphism (RFLP)\u003cbr\u003e- SNP (Single Nucleotide Polymorphism) analysis\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"800 rxns","offer_id":41528665604230,"sku":"E1031-A","price":84.0,"currency_code":"USD","in_stock":true},{"title":"2,500 rxns","offer_id":41528665636998,"sku":"E1031-B","price":256.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_815efb7b-1f3c-4b31-b6a2-921e5f2fea1e.jpg?v=1737093521"},{"product_id":"bsu15i","title":"Bsu15I","description":"\u003cdiv\u003e\u003cspan\u003e\u003cspan data-spm-anchor-id=\"a2700.details.0.i68.7482399bxP8YWN\"\u003eRecognition site:\u003cbr\u003e5' A T ↓ C G A T 3' \u003cbr\u003e3' T A G C ↑ T A 5'\u003cbr\u003e\u003cbr\u003eProduct Description \u003cbr\u003eThe Bsu15Ⅰ restriction endonuclease recognizes the AT^CGAT site and achieves optimal cutting efficiency within 5–15 minutes at 37°C using the universal buffer. Isoschizomers: ClaⅠ, BanⅢ, Bsa29Ⅰ, BseCⅠ, BshⅥ, BspDⅠ, BspXⅠ, BsuTUⅠ.\u003c\/span\u003e\u003cbr\u003eGDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.\u003cbr\u003eThe universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.\u003cbr\u003eThe Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003eStorage Condition \u0026amp; Shelf Life\u003cbr\u003eStore at -20°C.\u003cbr\u003e\u003cbr\u003eFeatures\u003cbr\u003e- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.\u003cbr\u003e- 100% buffer compatibility with downstream applications.\u003cbr\u003e- Enzymatic digestion can be completed within 5–15 minutes.\u003cbr\u003e- Direct loading onto a gel.\u003cbr\u003e- No star activity.\u003cbr\u003e\u003cbr\u003eScope of Application \u003cbr\u003e- Molecular cloning\u003cbr\u003e- Restriction site mapping\u003cbr\u003e- Genotyping\u003cbr\u003e- Southern blotting\u003cbr\u003e- Restriction fragment length polymorphism (RFLP)\u003cbr\u003e- SNP (Single Nucleotide Polymorphism) analysis\u003c\/span\u003e\u003c\/div\u003e","brand":"GDSBIO","offers":[{"title":"50 rxns","offer_id":41528665702534,"sku":"E1032-A","price":83.0,"currency_code":"USD","in_stock":true},{"title":"100 rxns","offer_id":41528665735302,"sku":"E1032-B","price":148.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_8c7844a3-7f96-47c5-bc9a-4706ba0b2eed.jpg?v=1737093524"},{"product_id":"esp3i","title":"Esp3I","description":"\u003cdiv\u003e\u003cspan\u003e\u003cspan data-spm-anchor-id=\"a2700.details.0.i66.2eac1caahgSFfz\"\u003eRecognition site:\u003cbr\u003e5' C G T C T C N1 ↓ 3'\u003cbr\u003e3' G C A G A G N5 ↑ 5'\u003cbr\u003e\u003cbr\u003eProduct Description \u003cbr\u003eThe Esp3Ⅰ restriction endonuclease recognizes the CGTCTC(1\/5)^ site and achieves optimal cutting efficiency within 5–15 minutes at 37°C using the universal buffer. Isoschizomers: BsmBⅠ, BstGZ53Ⅰ, Esp16Ⅰ, Esp23Ⅰ.\u003c\/span\u003e\u003cbr\u003eGDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.\u003cbr\u003eThe universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.\u003cbr\u003eThe Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003eStorage Condition\u003cbr\u003eStore at -20°C.\u003cbr\u003e\u003cbr\u003eFeatures\u003cbr\u003e- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.\u003cbr\u003e- 100% buffer compatibility with downstream applications.\u003cbr\u003e- Enzymatic digestion can be completed within 5–15 minutes.\u003cbr\u003e- Direct loading onto a gel.\u003cbr\u003e- No star activity.\u003cbr\u003e\u003cbr\u003eScope of Application \u003cbr\u003e- Molecular cloning\u003cbr\u003e- Restriction site mapping\u003cbr\u003e- Genotyping\u003cbr\u003e- Southern blotting\u003cbr\u003e- Restriction fragment length polymorphism (RFLP)\u003cbr\u003e- SNP (Single Nucleotide Polymorphism) analysis\u003c\/span\u003e\u003c\/div\u003e","brand":"GDSBIO","offers":[{"title":"20 rxns","offer_id":41528666718342,"sku":"E1033-A","price":147.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_ecd69c79-dac8-4ed0-aa1f-2dc5d9737957.jpg?v=1737093527"},{"product_id":"kpni","title":"KpnI","description":"\u003cdiv\u003e\u003cspan\u003e\u003cspan data-spm-anchor-id=\"a2700.details.0.i66.75bf1d35sAnCPI\"\u003eRecognition site:\u003cbr\u003e5' G G T A C ↓ C 3'\u003cbr\u003e3' C ↑ C A T G G 5'\u003cbr\u003e\u003cbr\u003eProduct Description \u003cbr\u003eThe KpnⅠ restriction endonuclease recognizes the GGTAC^C site and achieves optimal cutting efficiency within 5–15 minutes at 37°C using the universal buffer. Isoschizomer: Asp718Ⅰ.\u003c\/span\u003e\u003cbr\u003eGDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.\u003cbr\u003eThe universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.\u003cbr\u003eThe Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003eStorage Condition \u003cbr\u003eStore at -20°C.\u003cbr\u003e\u003cbr\u003eFeatures\u003cbr\u003e- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.\u003cbr\u003e- 100% buffer compatibility with downstream applications.\u003cbr\u003e- Enzymatic digestion can be completed within 5–15 minutes.\u003cbr\u003e- Direct loading onto a gel.\u003cbr\u003e- No star activity.\u003cbr\u003e\u003cbr\u003eScope of Application \u003cbr\u003e- Molecular cloning\u003cbr\u003e- Restriction site mapping\u003cbr\u003e- Genotyping\u003cbr\u003e- Southern blotting\u003cbr\u003e- Restriction fragment length polymorphism (RFLP)\u003cbr\u003e- SNP (Single Nucleotide Polymorphism) analysis\u003c\/span\u003e\u003c\/div\u003e","brand":"GDSBIO","offers":[{"title":"300 rxns","offer_id":41528666751110,"sku":"E1034-A","price":79.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_6c2fdfa1-2764-4256-bc55-af8c38ebe533.jpg?v=1737093530"},{"product_id":"ndei","title":"NdeI","description":"\u003cdiv\u003e\u003cspan\u003e\u003cspan data-spm-anchor-id=\"a2700.details.0.i62.290a1930jQrDYN\"\u003eRecognition site:\u003cbr\u003e5' C A ↓ T A T G 3' \u003cbr\u003e3' G T A T ↑ A C 5' \u003cbr\u003e\u003cbr\u003eProduct Description \u003cbr\u003eThe NdeⅠ restriction endonuclease recognizes the CA^TATG site and achieves optimal cutting efficiency within 5–15 minutes at 37°C using the universal buffer. Isoschizomer: FauNDⅠ.\u003c\/span\u003e\u003cbr\u003eGDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.\u003cbr\u003eThe universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.\u003cbr\u003eThe Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003eStorage Condition \u0026amp; Shelf Life\u003cbr\u003eStore at -20°C.\u003cbr\u003e\u003cbr\u003eFeatures\u003cbr\u003e- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.\u003cbr\u003e- 100% buffer compatibility with downstream applications.\u003cbr\u003e- Enzymatic digestion can be completed within 5–15 minutes.\u003cbr\u003e- Direct loading onto a gel.\u003cbr\u003e- No star activity.\u003cbr\u003e\u003cbr\u003eScope of Application \u003cbr\u003e- Molecular cloning\u003cbr\u003e- Restriction site mapping\u003cbr\u003e- Genotyping\u003cbr\u003e- Southern blotting\u003cbr\u003e- Restriction fragment length polymorphism (RFLP)\u003cbr\u003e- SNP (Single Nucleotide Polymorphism) analysis\u003c\/span\u003e\u003c\/div\u003e","brand":"GDSBIO","offers":[{"title":"100 rxns","offer_id":41528666849414,"sku":"E1035-A","price":52.0,"currency_code":"USD","in_stock":true},{"title":"300 rxns","offer_id":41528666882182,"sku":"E1035-B","price":94.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_2414e1fb-c345-431f-99c5-c9cd36f0b27f.jpg?v=1737093533"},{"product_id":"smai","title":"SmaI","description":"\u003cdiv\u003e\u003cspan\u003e\u003cspan data-spm-anchor-id=\"a2700.details.0.i67.39447e0cXAzhC8\"\u003eRecognition site:\u003cbr\u003e5' C C C ↓ G G G 3' \u003cbr\u003e3' G G G ↑ C C C 5'\u003cbr\u003e\u003cbr\u003eProduct Description \u003cbr\u003eThe SmaⅠ restriction endonuclease recognizes the CCC^GGG site and achieves optimal cutting efficiency within 5–15 minutes at 37°C using the universal buffer. Isoschizomer: TspMⅠ, XmaCⅠ, XmaⅠ.\u003c\/span\u003e\u003cbr\u003eGDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.\u003cbr\u003eThe universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.\u003cbr\u003eThe Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003eStorage Condition \u003cbr\u003eStore at -20°C.\u003cbr\u003e\u003cbr\u003eFeatures\u003cbr\u003e- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.\u003cbr\u003e- 100% buffer compatibility with downstream applications.\u003cbr\u003e- Enzymatic digestion can be completed within 5–15 minutes.\u003cbr\u003e- Direct loading onto a gel.\u003cbr\u003e- No star activity.\u003cbr\u003e\u003cbr\u003eScope of Application \u003cbr\u003e- Molecular cloning\u003cbr\u003e- Restriction site mapping\u003cbr\u003e- Genotyping\u003cbr\u003e- Southern blotting\u003cbr\u003e- Restriction fragment length polymorphism (RFLP)\u003cbr\u003e- SNP (Single Nucleotide Polymorphism) analysis\u003c\/span\u003e\u003c\/div\u003e","brand":"GDSBIO","offers":[{"title":"100 rxns","offer_id":41528666914950,"sku":"E1036-A","price":60.0,"currency_code":"USD","in_stock":true},{"title":"200 rxns","offer_id":41528666947718,"sku":"E1036-B","price":118.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_a30a7be0-bcf4-42ba-8efd-adc6da708aee.jpg?v=1737093536"},{"product_id":"saci","title":"SacI","description":"\u003cdiv class=\"J_module\"\u003e\n\u003cdiv class=\"detail-decorate-json-renderer-container\"\u003e\n\u003cdiv class=\"magic-1\"\u003e\n\u003cdiv class=\"magic-2\"\u003e\n\u003cdiv class=\"magic-3\"\u003e\n\u003cdiv class=\"magic-4\"\u003e\n\u003cdiv\u003e\u003cspan\u003e\u003cspan data-spm-anchor-id=\"a2700.details.0.i66.612320acZy1FWS\"\u003eRecognition site:\u003cbr\u003e5' G A G C T ↓ C 3' \u003cbr\u003e3' C ↑ T C G A G 5' \u003cbr\u003e\u003cbr\u003eProduct Description \u003cbr\u003eThe SacⅠ restriction endonuclease recognizes the GAGCT^C site and achieves optimal cutting efficiency within 5–15 minutes at 37°C using the universal buffer. Isoschizomers: Eco53kⅠ, EcoICRⅠ, Psp124BⅠ, SstⅠ.\u003c\/span\u003e\u003cbr\u003eGDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.\u003cbr\u003eThe universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.\u003cbr\u003eThe Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003cdiv\u003e\u003cspan\u003eStorage Condition\u003cbr\u003eStore at -20°C.\u003cbr\u003e\u003cbr\u003eFeatures\u003cbr\u003e- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.\u003cbr\u003e- 100% buffer compatibility with downstream applications.\u003cbr\u003e- Enzymatic digestion can be completed within 5–15 minutes.\u003cbr\u003e- Direct loading onto a gel.\u003cbr\u003e- No star activity.\u003cbr\u003e\u003cbr\u003eScope of Application \u003cbr\u003e- Molecular cloning\u003cbr\u003e- Restriction site mapping\u003cbr\u003e- Genotyping\u003cbr\u003e- Southern blotting\u003cbr\u003e- Restriction fragment length polymorphism (RFLP)\u003cbr\u003e- SNP (Single Nucleotide Polymorphism) analysis\u003cbr\u003e\u003c\/span\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"J_module\"\u003e\n\u003cdiv class=\"icbu-pc-images magic-5\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"flex-layout-v\"\u003e\n\u003cdiv class=\"flex-layout-h magic-5\"\u003e\u003cbr\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"GDSBIO","offers":[{"title":"100 rxns","offer_id":41528667013254,"sku":"E1037-A","price":61.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_71ece8ee-a22c-47a2-9e54-c9bc84774069.jpg?v=1737093539"},{"product_id":"bcui","title":"BcuI","description":"\u003cp\u003eRecognition site:\u003cbr\u003e5' A ∣ C T A G T 3' \u003cbr\u003e3' T G A T C ∥ A 5'\u003cbr\u003e \u003cbr\u003eProduct Description \u003cbr\u003eThe BcuI restriction endonuclease recognizes the A^CTAGT site and achieves optimal cutting efficiency within 5-15 minutes at 37\u003cspan\u003e°C\u003c\/span\u003e using the universal buffer. Isoschizomers: SpeI and AhlI.\u003cbr\u003eGDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.\u003cbr\u003eThe universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5-15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.\u003cbr\u003eThe Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.\u003c\/p\u003e\n\u003cp\u003eStorage Condition \u0026amp; Shelf Life\u003cbr\u003eStore at -20\u003cspan\u003e°C\u003c\/span\u003e.\u003cbr\u003e \u003cbr\u003eFeatures\u003cbr\u003e- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.\u003cbr\u003e- 100% buffer compatibility with downstream applications.\u003cbr\u003e- Enzymatic digestion can be completed within 5-15 minutes.\u003cbr\u003e- Direct loading onto a gel.\u003cbr\u003e- No star activity.\u003cbr\u003e \u003cbr\u003eScope of Application \u003cbr\u003e- Molecular cloning\u003cbr\u003e- Restriction site mapping\u003cbr\u003e- Genotyping\u003cbr\u003e- Southern blotting\u003cbr\u003e- Restriction fragment length polymorphism (RFLP)\u003cbr\u003e- SNP (Single Nucleotide Polymorphism) analysis\u003c\/p\u003e","brand":"GDSBio","offers":[{"title":"E1038-A\/50 rxns","offer_id":42313148366982,"sku":"E1038-A","price":128.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_4649c787-5da2-45b4-8e86-499ab660134b.jpg?v=1745743852"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/collections\/ngs_enzyme_0cee4ff4-2cb3-4e5a-8ef1-415f8ea05e42.jpg?v=1737093439","url":"https:\/\/www.gdsbio.com\/collections\/fast-restriction-endonuclease.oembed","provider":"GDSBio","version":"1.0","type":"link"}