{"title":"NGS Library Prep Enzyme","description":"\u003cp\u003eGDSBio provides advanced and innovative enzymes for DNA\/RNA library preparation for NGS.\u003c\/p\u003e","products":[{"product_id":"klenow-fragment","title":"Klenow Fragment（3´→5´ exo-）","description":"\u003cp\u003eKlenow Fragment （3´→5´ exo-）  is an N-terminal truncation of DNA Polymerase I which retains polymerase activity, but has lost the 5´→ 3´ exonuclease activity and has mutations which abolish the 3´→5´ exonuclease activity.\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003e1.Generates probes using random primers\u003cbr\u003e2.Random priming labeling\u003cbr\u003e3.dA-tailing at 3' end\u003cbr\u003e4.Second strand cDNA synthesis\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003eModerate strand displacement activity\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"K010-A\/100 U","offer_id":41420075532422,"sku":"K010-A","price":17.0,"currency_code":"USD","in_stock":true},{"title":"K010-B\/200 U","offer_id":41420075565190,"sku":"K010-B","price":29.0,"currency_code":"USD","in_stock":true},{"title":"K010-C\/1,000 U","offer_id":41420075597958,"sku":"K010-C","price":71.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_63f34ddc-b608-4408-a3f4-bc8946f703ee.jpg?v=1730706655"},{"product_id":"t4-dna-polymerase","title":"T4 DNA Polymerase","description":"\u003cp\u003eIn the presence of templates and primers, T4 DNA Polymerase catalyzes DNA synthesis in the 5→3 direction. This enzyme also has the activity of 3→5 exonuclease which is stronger than E. coli DNA polymerase I. Unlike DNA polymerase I, T4 DNA Polymerase does not have 5→3 exonuclease activity.\u003c\/p\u003e\n\u003cp\u003e5U\/µL\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003e1. Removal of 3'- overhangs to form blunt ends; \u003cbr\u003e2. Fill-in of 5'- overhangs to form blunt ends; \u003cbr\u003e3. Labeling DNA probe synthesis through displacement reaction; \u003cbr\u003e4. Synthesis of the second strand during site-directed mutagenesis; \u003cbr\u003e5. PCR product cloning without relying on ligation reactions.\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003e5' →3' polymerase activity\u003cbr\u003e3' →5' exonuclease activity\u003cbr\u003eNo 5' →3' exonuclease activity\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"K011-A\/100 U","offer_id":41420075630726,"sku":"K011-A","price":49.0,"currency_code":"USD","in_stock":true},{"title":"K011-B\/500 U","offer_id":41420075663494,"sku":"K011-B","price":185.0,"currency_code":"USD","in_stock":true},{"title":"K011-C\/2,000 U","offer_id":41420075696262,"sku":"K011-C","price":523.0,"currency_code":"USD","in_stock":true},{"title":"K011-D\/5,000 U","offer_id":41420075729030,"sku":"K011-D","price":977.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_1ffe31bc-0a83-4b51-8294-0dc2a0701703.jpg?v=1730706657"},{"product_id":"t4-dna-ligase-fast","title":"T4 DNA Ligase (Fast)","description":"\u003cp\u003eT4 DNA Ligase (Fast) is produced by E. coli carrying T4 bacteriophage gene 30. This enzyme catalyzes the formation of phosphodiester bonds between the 5'-phosphate group and the 3'-hydroxyl group of double-helix DNA or RNA. This enzyme can repair single-strand breaks in double-stranded DNA, RNA, or DNA\/RNA complexes, and can connect DNA fragments with sticky or blunt ends, but it is inactive for single-stranded nucleic acids. It is mainly used for cloning of restriction enzyme digestion products, gene site-directed mutagenesis, PCR product cloning, linear DNA self-looping, and repair of double-stranded DNA breaks. T4 DNA Ligase (Fast) requires ATP as a cofactor and completes the sticky end ligation reaction at room temperature in only 10 minutes.\u003c\/p\u003e\n\u003cp\u003econcentration: 5U\/µL\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003e1.TA clone\u003cbr\u003e2.NGS adapter ligation\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003eHigh ligation activity\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"1,000 U","offer_id":41420075761798,"sku":"K012","price":105.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_bb38757d-7050-44e4-83fe-b12b02547583.jpg?v=1730706660"},{"product_id":"t4-polynucleotide-kinase","title":"T4 Polynucleotide Kinase","description":"\u003cp\u003eT4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer and exchange of Pi from the γ  position of ATP to the 5'-hydroxyl terminus of polynucleotides (double-stranded and single-stranded DNA and RNA) and nucleoside 3'-monophosphate. T4 polynucleotide kinase also catalyzes the removal of 3'-phosphoryl groups from 3'-phosphate-based polynucleotides, deoxynucleoside 3'-monophosphate, and deoxynucleoside 3' -diphosphate. When ADP is present, T4 PNK has 5'-end phosphatase activity, catalyzing the exchange of 5'-P-oligomeric\/polymeric nucleotides and ATP terminal 5'-phosphate groups. It is suitable for constructing DNA libraries and producing probes with terminal labeling.\u003c\/p\u003e\n\u003cp\u003e10U\/µL\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003e1. End labeling DNA or RNA for probes and DNA sequencing; \u003cbr\u003e2. 5' phosphorylation of DNA\/RNA for subsequent ligation; \u003cbr\u003e3. Phosphorylate the 5' end of the 3' phosphorylated mononucleotide to prepare the pNp substrate for addition to the 3' end of DNA or RNA; \u003cbr\u003e4. Label the 5' end of oligonucleotides with a phosphate group at the 3' end.\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003ePhosphorylation of the 5' end of DNA or RNA for ligation reactions\u003cbr\u003eTerminal labeling of DNA or RNA for use as probes and DNA sequencing\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"50 U","offer_id":41420075794566,"sku":"K013-A","price":49.0,"currency_code":"USD","in_stock":true},{"title":"2500 U","offer_id":41420075827334,"sku":"K013-B","price":132.0,"currency_code":"USD","in_stock":true},{"title":"10,000 U","offer_id":41420075860102,"sku":"K013-C","price":391.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_37df291e-c0c3-4a43-823b-60f90ab1741a.jpg?v=1730706662"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/collections\/ngs_enzyme.jpg?v=1737282635","url":"https:\/\/www.gdsbio.com\/collections\/ngs-library-prep-enzyme.oembed","provider":"GDSBio","version":"1.0","type":"link"}