{"title":"NGS Library Prep Module","description":"\u003cp\u003eGDSBio provides advanced and innovative modules for DNA\/RNA library preparation for NGS.\u003c\/p\u003e","products":[{"product_id":"gds-rna-first-strand-synthesis-module","title":"GDS RNA First Strand Synthesis Module","description":"\u003cp\u003eThe GDSNext Non-Directional RNA Second Strand Synthesis Module, an optimized non-specific RNA second-strand synthesis module, can utilize the first-strand cDNA synthesized by the GDSNext RNA First Strand Synthesis Module (# K020) to generate double-stranded cDNA. The dsDNA produced by the GDSNext Non-Directional RNA Second Strand Synthesis Module can then be converted into blunt-end DNA fragments using the GDSNext End Repair\/dA-Tailing Module (# K025).\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"24 rxns","offer_id":41420091326598,"sku":"K020-A","price":194.0,"currency_code":"USD","in_stock":true},{"title":"96 rxns","offer_id":41420091359366,"sku":"K020-B","price":600.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/KU001_64c57fe5-69e0-4c1d-8cb6-bafe01f54994.jpg?v=1730707847"},{"product_id":"gds-directional-rna-second-strand-synthesis-module","title":"GDS Directional RNA Second Strand Synthesis Module","description":"\u003cp\u003eThe GDSNext Directional RNA Second Strand Synthesis Module, a specific RNA second-strand synthesis module, has been optimized to generate double-stranded cDNA using the first-strand cDNA synthesized by the GDSNext RNA First Strand Synthesis Module (# K020). The dsDNA produced by the GDSNext Directional RNA Second Strand Synthesis Module can then be converted into blunt-end DNA fragments using the GDSNext End Repair\/dA-Tailing Module (# K025).\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"K021-A\/24 rxns","offer_id":41420091392134,"sku":"K021-A","price":492.0,"currency_code":"USD","in_stock":true},{"title":"K021-B\/96 rxns","offer_id":41420091424902,"sku":"K021-B","price":1690.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/KU001_c16d32c3-3c17-41b1-b923-88f4fd92307f.jpg?v=1730707850"},{"product_id":"gds-non-directional-rna-second-strand-synthesis-module","title":"GDS Non-Directional RNA Second Strand Synthesis Module","description":"\u003cp\u003eThe GDSNext Non-Directional RNA Second Strand Synthesis Module, an optimized non-specific RNA second-strand synthesis module, can utilize the first-strand cDNA synthesized by the GDSNext RNA First Strand Synthesis Module (# K020) to generate double-stranded cDNA. The dsDNA produced by the GDSNext Non-Directional RNA Second Strand Synthesis Module can then be converted into blunt-end DNA fragments using the GDSNext End Repair\/dA-Tailing Module (# K025).\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"20 rxns","offer_id":41420091457670,"sku":"K022-A","price":415.0,"currency_code":"USD","in_stock":true},{"title":"100 rxns","offer_id":41420091490438,"sku":"K022-B","price":1770.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/KU001_17e6709c-e69c-447a-aea5-d9d9902b4757.jpg?v=1730707852"},{"product_id":"gds-fragmentation-end-prep-module","title":"GDS Fragmentation \u0026 End Prep Module","description":"\u003cp\u003eGDS Fragmentation \u0026amp; End Prep Module is designed for library construction of high-throughput sequencing platform. The module combines fragmentation of intact DNA, end repair and dA tailing, reducing operation and reaction time and the amount of template. 100 pg ~ 500 ng DNA template can be efficiently fragmented with 5'- phosphorylated 3' dA-tailed ends. This product contains the enzymes and buffers required for the reaction, easy to use, and compatible with automatic library preparation equipment.\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003eFragmentation, end repair and dA-tailing in dsDNA library construction.\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003eRapid and simultaneous completion of DNA fragmentation, end repair, and dA tailing\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"24 rxns","offer_id":41420091523206,"sku":"K023-A","price":346.0,"currency_code":"USD","in_stock":true},{"title":"96 rxns","offer_id":41420091555974,"sku":"K023-B","price":1100.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/KU001_fcf064e4-c55c-4cd2-a7f1-0bc1ac1a38fb.jpg?v=1730707855"},{"product_id":"gds-end-preparation-module","title":"GDS End Preparation Module","description":"\u003cp\u003eThe GDS End Preparation Module is an end-repair\/dA-tailing module for NGS library preparation, and has been optimized to convert 500 pg-1 µg of fragmented DNA to repaired DNA having 5' phosphorylated, 3' dA-tailed ends.\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003eConvert 500 pg-1µg of fragmented DNA to repaired DNA with 5' phosphorylated, 3' dA-tailed ends.\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003eRapid and simultaneous completion of DNA end repair and dA tailing\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"K025-A\/24 rxns","offer_id":41420092473478,"sku":"K025-A","price":260.0,"currency_code":"USD","in_stock":true},{"title":"K025-B\/96 rxns","offer_id":41420092506246,"sku":"K025-B","price":890.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/KU001_bce2c5a0-4efe-4642-947d-6d3bac732525.jpg?v=1730707859"},{"product_id":"gds-ligation-module","title":"GDS Ligation Module","description":"\u003cp\u003eThe GDS Ligation Module has been optimized to ligate efficiently DNA adaptors compatible with Illumina sequencing to end-repaired, dA-tailed DNA fragments. The module is optimized for use with the GDS End Preparation Module (#K025). This module enables high yield preparation of high quality libraries from 500 pg to 1µg of input DNA.\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003eAdapter ligation in NGS library preparation.\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003eHigh ligation efficiency\u003cbr\u003eWide range of input DNA: 500 pg-1 µg\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"24 rxns","offer_id":41420092571782,"sku":"K026-A","price":430.0,"currency_code":"USD","in_stock":true},{"title":"96 rxns","offer_id":41420092604550,"sku":"K026-B","price":1480.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/KU001_bb09e357-3709-4ee0-bdd0-caa191e831cd.jpg?v=1730707861"},{"product_id":"gds-fast-fragmentation-end-prep-module","title":"GDS Fast Fragmentation \u0026 End Prep Module","description":"\u003cp\u003eGDS Fast Fragmentation \u0026amp; End Prep Module is designed for library construction of high-throughput sequencing platform. The module combines fragmentation of intact DNA, end repair and dA tailing in 8 minutes. Not only the reaction time is minimized, but also the cutting efficiency of trace template is increased. 100 pg ~ 500 ng DNA template can be efficiently fragmented with 5'- phosphorylated 3' dA-tailed ends. This product contains the enzymes and buffers required for the reaction, easy to use, and compatible with automatic library preparation equipment.\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003eFragmentation, end repair and dA-tailing in dsDNA library construction.\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003eRapid and simultaneous completion of DNA fragmentation, end repair, and dA tailing\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"K032-A\/24 rxns","offer_id":41420092637318,"sku":"K032-A","price":398.0,"currency_code":"USD","in_stock":true},{"title":"K032-B\/96 rxns","offer_id":41420092670086,"sku":"K032-B","price":1265.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/KU001_fa2a3377-f505-4791-af76-e0e0908d1fa3.jpg?v=1730707864"},{"product_id":"picogreen-dsdna-quantitation-reagent","title":"PicoGreen dsDNA Quantitation Reagent","description":"\u003cp\u003ePicoGreen is a highly sensitive fluorescence detection reagent specifically designed for the quantitation analysis of double-stranded DNA (dsDNA). It is widely used in molecular biology research and bioproduct testing due to its excellent sensitivity, specificity, and convenience. PicoGreen only emits fluorescence upon binding to dsDNA, and the fluorescence intensity is proportional to the DNA concentration, making it an ideal choice for quantifying dsDNA.\u003c\/p\u003e\n\n\u003cp\u003eApplication\u003cbr\u003e\nQuantitation of PCR products, genomic DNA quantitation, determination of dsDNA in complex mixtures, viral DNA quantitation, etc.\u003c\/p\u003e\n\n\u003cp\u003eFeatures\u003cbr\u003e\nHigh Sensitivity: Can detect dsDNA in the range of 25 pg\/mL to 1000 ng\/mL with a good linear relationship (R2\u0026gt;0.99).\u003cbr\u003e\nEasy Operation: The quantitation method is simple and quick, easy to perform in the laboratory.\u003cbr\u003e\nStrong Anti-interference Ability: Basically unaffected by single-stranded DNA (ssDNA) and RNA, can tolerate certain concentrations of salts, urea, ethanol, chloroform, detergents, proteins, and other interferences.\u003cbr\u003e\nWide Linear Range: The fluorescence response has a broad linear range, covering multiple orders of magnitude.\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"1 mL","offer_id":41460425654406,"sku":"P6021","price":412.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/M7021-1_164b0382-716a-4289-a036-e840e02814e0.jpg?v=1732870565"},{"product_id":"gds-ds-cdna-synthesis-module-gdna-wiper","title":"GDS ds-cDNA Synthesis Module (gDNA wiper+)","description":"\u003cp\u003eThe GDS ds-cDNA Synthesis Module (gDNA wiper+) is a cDNA synthesis kit for RNA sequencing library preparation on Illumina and MGI platforms. It integrates efficient genomic DNA removal and high-quality double-stranded cDNA synthesis. The generated cDNA can be directly used for subsequent NGS library construction.\u003c\/p\u003e\n\u003cp\u003eApplication Scope:\u003cbr\u003eFor RNA reverse transcription and double-stranded cDNA synthesis during NGS library preparation.\u003c\/p\u003e\n\u003cp\u003eProduct Features:\u003cbr\u003eIntegrated genomic DNA removal and cDNA synthesis in one workflow.\u003cbr\u003eSuitable for Illumina and MGI sequencing platforms.\u003cbr\u003eEfficient removal of contaminating gDNA, improving library quality.\u003cbr\u003eHigh yield and reliable synthesis of double-stranded cDNA.\u003cbr\u003eSimple and streamlined operation.\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"K036-A\/24 rxns","offer_id":41758604099718,"sku":"K036-A","price":706.0,"currency_code":"USD","in_stock":true},{"title":"K036-B\/48 rxns","offer_id":41758604132486,"sku":"K036-B","price":1380.0,"currency_code":"USD","in_stock":true},{"title":"K036-C\/96 rxns","offer_id":41758604165254,"sku":"K036-C","price":2300.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/KU001_9fac964c-91f5-4067-a531-99b192867779.jpg?v=1745743874"},{"product_id":"gds-dsdna-fragmentation-enzyme","title":"GDS dsDNA Fragmentation Enzyme","description":"\u003cp\u003eGDS dsDNA Fragmentation Enzyme, a double-stranded DNA fragmentation enzyme, produces dsDNA breaks in a time-dependent manner, producing DNA fragments of 50-1,000 bp depending on reaction time. The GDS dsDNA Fragmentation Enzyme consists of two enzymes, one that randomly generates nicks on dsDNA and the other recognizes the nicked sites and cuts the opposite DNA strand aross from the nick, thus producing dsDNA breaks. The resulting DNA fragment contains short overhangs, 5' -phosphate, and 3' -hydroxyl groups. The random nicking activity of GDS dsDNA Fragmentation Enzyme has been demonstrated by the preparation of next-generation sequencing libraries. The comparison of the sequencing results between libraries prepared by genomic DNA sheared by GDS dsDNA Fragmentation Enzyme with mechanically shearing showed that GDS dsDNA Fragmentation Enzyme does not introduce any detectable bias in the preparation of sequencing libraries, and there is no difference in sequence coverage between the two methods.\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003eFragmentation of dsDNA.\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003eRandom fragmentation can be achieved by adjusting the incubation time\u003c\/p\u003e","brand":"GDSBio","offers":[{"title":"K024-A\/50 rxns","offer_id":44057030688902,"sku":"K024-A","price":246.0,"currency_code":"USD","in_stock":true},{"title":"K024-B\/250 rxns","offer_id":44057030721670,"sku":"K024-B","price":920.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/P4041b-2.png?v=1779761078"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/collections\/KU001_fa2a3377-f505-4791-af76-e0e0908d1fa3.jpg?v=1730708059","url":"https:\/\/www.gdsbio.com\/collections\/ngs-library-prep-module.oembed","provider":"GDSBio","version":"1.0","type":"link"}