{"title":"RT \u0026 RT-PCR\/qPCR","description":"\u003cp\u003eGDSBio provides high quality reagents related to RNA reverse transcription, one-step reverse transcription and PCR\/qPCR for RNA research.\u003c\/p\u003e","products":[{"product_id":"2x-one-step-rt-pcr-mix-1-tube","title":"2X One Step RT-PCR Mix 1-tube","description":"\u003cp\u003e2X One Step RT-PCR Mix is a one-step kit for reverse transcription and endpoint PCR. It amplifies RNA target sequences with gene-specific primers (GSP). Optimized buffer components enable reverse transcription and PCR reactions to be performed within one system. The mixture is provided in a single tube at 2X concentration, which is simple and convenient to use, only GSP and template RNA need to be added to use, and no additional capping and pipetting operations are required, which not only saves time, but also effectively reduces the risk of contamination. This product contains heat labile DNase, which can remove genomic DNA at the same time when reverse transcription. Heat labile DNase is susceptible to high temperature inactivation and is conducive to stable preservation of cDNA or amplification reactions.\u003c\/p\u003e\n\n\u003cp\u003eApplication\u003cbr\u003e\n1-STEP reverse transcription and endpoint PCR\u003c\/p\u003e\n\n\u003cp\u003eFeatures\u003cbr\u003e\nAll-in-one master mix, easy to use and saving time\u003cbr\u003e\nHeat labile DNase, remove genomic DNA and be inactivated thoroughly at high temperature of PCR\u003cbr\u003e\nHigh efficiency of reverse transcription and PCR\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"RP1001\/50 rnxs","offer_id":41401460293766,"sku":"RP1001","price":110.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/RP1001-1_b702715c-0688-4e75-b818-256d0c31e2dd.jpg?v=1729479376"},{"product_id":"2x-one-step-rt-pcr-mix-2-tube","title":"2X One Step RT-PCR Mix 2-tube","description":"\u003cp\u003e2X One Step RT-PCR Mix is a one-step kit for reverse transcription and endpoint PCR. It amplifies RNA target sequences with gene-specific primers (GSP). Optimized buffer components enable reverse transcription and PCR reactions to be performed within one system. This product is composed of a two-fold concentration of reaction buffer and an enzyme mixture, which is more conducive to the stable preservation of enzyme reagents than a one-tube all-in mix. This product is simple and convenient to use, only GSP and template RNA need to be added to use, and no additional capping and pipetting operations are required, which not only saves time, but also effectively reduces the risk of contamination. This product contains heat labile DNase, which can remove genomic DNA at the same time when reverse transcription. Heat labile DNase is susceptible to high temperature inactivation and is conducive to stable preservation of cDNA or amplification reactions.\u003c\/p\u003e\n\n\u003cp\u003eApplication\u003cbr\u003e\n1-STEP reverse transcription and endpoint PCR\u003c\/p\u003e\n\n\u003cp\u003eFeatures\u003cbr\u003e\nEnzyme mix + reaction mix, the amount of enzyme can be flexibly adjusted\u003cbr\u003e\nHeat labile DNase, remove genomic DNA and be inactivated thoroughly at high temperature of PCR\u003cbr\u003e\nHigh efficiency of reverse transcription and PCR\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"RP1001B\/50 rxns","offer_id":41401460326534,"sku":"RP1001B","price":110.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/RP1001B_75a5fdbf-d589-48e6-8bb9-0d37282cdf4f.jpg?v=1729479379"},{"product_id":"one-step-probe-rt-qpcr-kit","title":"One-step Probe RT-qPCR Kit","description":"\u003cp\u003eOne-step Probe RT-qPCR Kit is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening\/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP\/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55℃, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Integrating the superior performance of Reverse Transcriptase and hot-start Taq DNA Polymerase, with the optimized buffer system, the detection sensitivity of One-step Probe RT-qPCR Kit can reach 0.1 pg total RNA or \u0026lt;10 copies of RNA template. The kit is available as a convenient Master Mix. 2X One-step U+ Mix contains an optimized buffer system and dNTP\/dUTP Mix, which is suitable for high-specificity detection systems with fluorescent labeled probes such as TaqMan?. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003eRNA virus \u0026amp; pathogen detection – e.g., SARS-CoV-2, HIV, influenza, dengue, HCV\u003cbr\u003eGene expression quantification – from low-abundance or precious RNA samples\u003cbr\u003eMultiplex target detection – simultaneous detection of 4–6 targets in one tube\u003cbr\u003eDirect amplification (no RNA extraction) – using crude lysates from swabs, blood, tissues\u003cbr\u003eLow-abundance \u0026amp; high-GC content RNA templates – high sensitivity down to a few copies or 0.1 pg total RNA\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003eOne-step RT-qPCR\u003cbr\u003eThis kit is suitable for fluorescence quantification by probe method\u003cbr\u003eThis kit is compatible with many real-time systems\u003cbr\u003eHot-start technology brings high specificity and reproducible amplification\u003cbr\u003edUTP\/UDG system, effectively prevent PCR product contamination\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/20260127172412.png?v=1769678791\"\u003e\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"V5001\/200 rxns","offer_id":41401653657734,"sku":"V5001","price":200.0,"currency_code":"USD","in_stock":true},{"title":"V5002\/5000 rxns","offer_id":41401653690502,"sku":"V5002","price":4000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/V5001-1_07c138f6-378c-4036-beba-be9cf8c4c4ca.jpg?v=1729499757"},{"product_id":"dspath-4x-one-step-multiplex-master-mix","title":"DSPath 4X One-Step Multiplex Master Mix","description":"\u003cp style=\"text-align: left;\"\u003eDSPath 4X One-Step Multiplex Master Mix is used to perform one-step multiplex real-time PCR applications with any gene-specific primer and probe sets, and is suitable for both RNA and DNA targets. This master mix is formulated with optimized buffer components to accommodate multiplex amplification of up to four RNA or DNA target sequences in a single reaction. The master mix is supplied at a 4X concentration that allows to input more sample into each reaction, increasing sensitivity even in low-volume reactions.\u003cbr\u003eRobust one-step, all-in-one master mix system for easy reaction assembly\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003cbr\u003eProbe gene expression analysis\u003cbr\u003eProbe Low-copy gene detection\u003cbr\u003eProbe microarray validation\u003cbr\u003eProbe gene knockdown validation\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003cbr\u003eDetect multiple targets in one reaction\u003cbr\u003eHigh sensitivity to detect low-copy targets\u003cbr\u003eTolerance of inhibitors commonly found in clinical samples\u003cbr\u003eEliminates the risk of cross contamination associated with two-step RT-qPCR protocols\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/8.png?v=1776051922\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\" align=\"center\"\u003eFigure 1. One-Step RT-qPCR workflow using DSPathTM 4X One-Step Multiplex Master Mix\u003c\/p\u003e\n\u003cp style=\"text-align: left;\" align=\"center\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/1.png?v=1776051680\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003eFigure 2. detection of low SARS-CoV-2 sample\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/2.png?v=1776051683\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003eFigure 3. detection of high SARS-CoV-2 sample\u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/1.png?v=1776051680\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\" align=\"center\"\u003eFigure 4. amplification curve of stability test of DSPath 4X One-Step Multiplex Master Mix\/MixB\/MixC\/MixD \u003c\/p\u003e\n\u003cp style=\"text-align: left;\" align=\"center\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: left;\" align=\"center\"\u003eFigure 5. Ct value of stability test of DSPath 4X One-Step Multiplex Master Mix\/MixB\/MixC\/MixD:\u003c\/p\u003e\n\u003cp style=\"text-align: left;\" align=\"center\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/4.png?v=1776051913\" alt=\"\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/5.png?v=1776051914\" alt=\"\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/6.png?v=1776051916\" alt=\"\"\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/7.png?v=1776051919\" alt=\"\"\u003e\u003c\/p\u003e\n\u003cp style=\"text-align: left;\" align=\"center\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e\n\u003cp style=\"text-align: left;\"\u003e \u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"V5005\/200 rxns","offer_id":41401653854342,"sku":"V5005","price":300.0,"currency_code":"USD","in_stock":true},{"title":"V5006\/5000 rxns","offer_id":41401653887110,"sku":"V5006","price":7500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/V5005-1_cb0dc9f9-2545-4a13-9006-ce109f155841.jpg?v=1776061048"},{"product_id":"dspath-4x-one-step-multiplex-master-mix-high-rox","title":"DSPath 4X One-Step Multiplex Master Mix (High ROX+)","description":"\u003cp\u003eDSPath 4X One-Step Multiplex Master Mix (High ROX+) is used to perform one-step multiplex real-time PCR applications with any gene-specific primer and probe sets in quantitative instruments that require high concentrations of ROX, and is suitable for both RNA and DNA targets. This master mix is formulated with optimized buffer components to accommodate multiplex amplification of up to four RNA or DNA target sequences in a single reaction. The master mix is supplied at a 4X concentration that allows to input more sample into each reaction, increasing sensitivity even in low-volume reactions.\u003c\/p\u003e\n\n\u003cp\u003eApplication\u003cbr\u003e\nProbe gene expression analysis\u003cbr\u003e\nProbe Low-copy gene detection\u003cbr\u003e\nProbe microarray validation\u003cbr\u003e\nProbe gene knockdown validation\u003c\/p\u003e\n\n\u003cp\u003eFeatures\u003cbr\u003e\nDetect multiple targets in one reaction\u003cbr\u003e\nHigh sensitivity to detect low-copy targets\u003cbr\u003e\nTolerance of inhibitors commonly found in clinical samples\u003cbr\u003e\nEliminates the risk of cross contamination associated with two-step RT-qPCR protocols\u003cbr\u003e\nPremixed with high concentration of ROX reference dye\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"V5005H\/200 rxns","offer_id":41401653919878,"sku":"V5005H","price":300.0,"currency_code":"USD","in_stock":true},{"title":"V5006H\/5000 rxns","offer_id":41401653952646,"sku":"V5006H","price":7500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/V5005H-1_6b0f66d1-3349-4e46-8f4a-03df282813fa.jpg?v=1729499764"},{"product_id":"dspath-4x-one-step-multiplex-master-mix-lyophilized","title":"DSPath 4X One-Step Multiplex Master Mix (Lyophilized)","description":"\u003cp\u003eDSPath 4X One-Step Multiplex Master Mix is used to perform one-step multiplex real-time PCR applications with any gene-specific primer and probe sets, and is suitable for both RNA and DNA targets. This master mix is formulated with optimized buffer components to accommodate multiplex amplification of up to four RNA or DNA target sequences in a single reaction. The master mix is supplied at a 4X concentration that allows to input more sample into each reaction, increasing sensitivity even in low-volume reactions. Lyophilized Enzyme Mix is a freeze-dried reagent made of  heat-resistant reverse transcriptase, hot-start Taq DNA polymerase, RNase inhibitor, Heat-labile UDG, dNTPs, etc., which makes the product performance more stable and can be stored for a long time.\u003c\/p\u003e\n\n\u003cp\u003eApplication\u003cbr\u003e\nProbe gene expression analysis\u003cbr\u003e\nProbe Low-copy gene detection\u003cbr\u003e\nProbe microarray validation\u003cbr\u003e\nProbe gene knockdown validation\u003c\/p\u003e\n\n\u003cp\u003eFeatures\u003cbr\u003e\nLyophilized powder, transportable and storable at room temperature\u003cbr\u003e\nOne-step RT-qPCR\u003cbr\u003e\nThis kit is suitable for fluorescence quantification by probe method\u003cbr\u003e\nThis kit is compatible with many real-time systems\u003cbr\u003e\nHot-start technology brings high specificity and reproducible amplification\u003cbr\u003e\ndUTP\/UDG system, effectively prevent PCR product contamination\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"V5005L\/200 rxns","offer_id":41401653985414,"sku":"V5005L","price":443.0,"currency_code":"USD","in_stock":true},{"title":"V5006L\/5000 rxns","offer_id":41401654018182,"sku":"V5006L","price":8615.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/V5005L-1_f69903a0-f4b7-4a08-94a4-ab8b0538cef3.jpg?v=1729499768"},{"product_id":"one-step-probe-rt-qpcr-kit-v2","title":"One-step Probe RT-qPCR Kit V2","description":"\u003cp\u003eOne-step Probe RT-qPCR Kit V2 is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening\/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP\/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55¡ãC, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Thermostable reverse transcriptase can maintain stable activity at 55¡ãC. The hot-start antibody-modified fast DNA polymerase and optimized buffer system ensure the extremely high sensitivity and specificity of the One-Step Probe RT-qPCR Kit V2. The kit is available as a convenient Master Mix. 2X RT-qPCR Mix contains an optimized buffer system and dNTP\/dUTP Mix, which is suitable for high-specificity detection systems with fluorescent labeled probes such as TaqMan.\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003eRNA virus \u0026amp; pathogen detection – e.g., SARS-CoV-2, HIV, influenza, dengue, HCV\u003cbr\u003eGene expression quantification – from low-abundance or precious RNA samples\u003cbr\u003eMultiplex target detection – simultaneous detection of 4–6 targets in one tube\u003cbr\u003eDirect amplification (no RNA extraction) – using crude lysates from swabs, blood, tissues\u003cbr\u003eLow-abundance \u0026amp; high-GC content RNA templates – high sensitivity down to a few copies or 0.1 pg total RNA\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003eFast start-up, 30s hot start\u003cbr\u003eReverse transcription at 55 ¡ãC to accommodate complex RNA templates\u003cbr\u003eContains heat-labile UDG to reduce false-positive contamination\u003cbr\u003eHigh sensitivity for detection of low-abundance genes\u003cbr\u003eHigh stability, unchanged performance at 4¡ãC and 37¡ãC for one week\u003cbr\u003eThe reaction system can be formulated up to 30 minutes in advance without changing performance\u003cbr\u003eSaves time by supporting fast program\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/20260127172412.png?v=1769678791\" alt=\"\"\u003e\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"V5009\/200 rxns","offer_id":41401654050950,"sku":"V5009","price":320.0,"currency_code":"USD","in_stock":true},{"title":"V5010\/5000 rxns","offer_id":41401654083718,"sku":"V5010","price":4462.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/V5009-1_ea0f8295-7d0b-4701-b5b0-9be2dc8221d3.jpg?v=1729499771"},{"product_id":"one-step-probe-rt-qpcr-kit-v3","title":"One-step Probe RT-qPCR Kit V3","description":"\u003cp\u003eOne-step Probe RT-qPCR Kit V3 is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening\/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP\/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55\u003cspan\u003e℃\u003c\/span\u003e, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Integrating the superior performance of Reverse Transcriptase and hot-start Taq DNA Polymerase, with the optimized buffer system, the detection sensitivity of One-step Probe RT-qPCR Kit can reach 0.1 pg total RNA or \u0026lt;10 copies of RNA template. The kit is available as a convenient Master Mix. 5X One-step U+ Mix contains an optimized buffer system and dNTP\/dUTP Mix, which is suitable for high-specificity, low template concentration and multiple detection systems with fluorescent labeled probes such as TaqMan. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003eRNA virus \u0026amp; pathogen detection – e.g., SARS-CoV-2, HIV, influenza, dengue, HCV\u003cbr\u003eGene expression quantification – from low-abundance or precious RNA samples\u003cbr\u003eMultiplex target detection – simultaneous detection of 4–6 targets in one tube\u003cbr\u003eDirect amplification (no RNA extraction) – using crude lysates from swabs, blood, tissues\u003cbr\u003eLow-abundance \u0026amp; high-GC content RNA templates – high sensitivity down to a few copies or 0.1 pg total RNA\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003eFast start-up, 30s hot start\u003cbr\u003eReverse transcription at 55\u003cspan\u003e℃\u003c\/span\u003e to accommodate complex RNA templates\u003cbr\u003eContains heat-labile UDG to reduce false-positive contamination\u003cbr\u003eHigh sensitivity for detection of low-abundance genes\u003cbr\u003eHigh stability, unchanged performance at 4\u003cspan\u003e℃\u003c\/span\u003e and 37\u003cspan\u003e℃\u003c\/span\u003e for one week\u003cbr\u003eThe reaction system can be formulated up to 30 minutes in advance without changing performance\u003cbr\u003eSaves time by supporting fast program\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/20260127172412.png?v=1769678791\" alt=\"\"\u003e\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"V5011\/200 rxns","offer_id":41401654149254,"sku":"V5011","price":351.0,"currency_code":"USD","in_stock":true},{"title":"V5011-2\/1000 rxns","offer_id":41401654182022,"sku":"V5011-2","price":1228.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/V5011-1_50b8c22b-dbc9-4f8c-85a8-5948a5e0f1d4.jpg?v=1729499775"},{"product_id":"super-4x-one-step-multiplex-master-mix","title":"Super 4X One-Step Multiplex Master Mix","description":"\u003cp\u003eSuper 4X One-Step Multiplex Master Mix is used to perform one-step multiplex real-time PCR applications with any gene-specific primer and probe sets, and is suitable for both RNA and DNA targets. Dual hot-start reverse transcriptase and DNA polymerase can complete more than 10-plex highly sensitive and specific quantitative PCR in a single system. The master mix is supplied at a 4X concentration that allows to input more sample into each reaction, increasing sensitivity even in low-volume reactions.\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003eUltra‑multiplex pathogen detection – Detects \u0026gt;10 RNA\/DNA targets in one tube (e.g., respiratory viruses, bloodborne pathogens).\u003cbr\u003eHigh sensitivity with low‑abundance targets – 4X concentration allows more sample input, ideal for liquid biopsy, low‑copy viruses, or precious specimens.\u003cbr\u003eGene expression \u0026amp; biomarker panels – Simultaneously quantifies multiple mRNAs (e.g., cytokines, oncogenes) from limited RNA samples.\u003cbr\u003eSmall‑volume \u0026amp; high‑throughput qPCR – Works in 5‑10 µL reactions, suitable for 384‑well plates, microfluidics, or portable qPCR devices.\u003cbr\u003eDirect amplification from crude samples – Tolerates inhibitors, enabling one‑step testing from swabs, blood lysates, or environmental samples without RNA extraction.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003esupport 10-plex amplification with high specificity\u003cbr\u003eReverse transcription at 55 ¡ãC to accommodate complex RNA templates\u003cbr\u003eContains heat-labile UDG to reduce false-positive contamination\u003cbr\u003eHigh sensitivity for detection of low-abundance genes\u003cbr\u003eHigh stability\u003cbr\u003e4X formation, high concentration\u003cbr\u003eSaves time by supporting fast program\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/20260127172412.png?v=1769678791\" alt=\"\"\u003e\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"V5012-A\/200 rxns","offer_id":41401654214790,"sku":"V5012-A","price":475.0,"currency_code":"USD","in_stock":true},{"title":"V5012-B\/5000 rxns","offer_id":41401654247558,"sku":"V5012-B","price":9350.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/V5012-1_3c0dab66-71f2-4317-9b40-a427677ec6bd.jpg?v=1729499778"},{"product_id":"gdslyo-one-step-probe-rt-qpcr-kit","title":"GDSlyo One-step Probe RT-qPCR Kit","description":"\u003cp\u003eGDSlyo One-step Probe RT-qPCR Kit is a glycerol-free one-step RT-qPCR reagent containing lyophilized excipients for the detection of RNA templates that can be lyophilized with a suitable lyophilization process. The difficulties in the development of lyophilized products include three aspects:1. The removal of liquid reagents affects the lyophilized components, and the stability is poor; 2. It is difficult to freeze-dry and mold, and the yield rate is low; 3. Poor long-term storage stability of lyophilized finished products. Through the optimization of functional proteins, the matching and screening of excipients and lyophilization processes, this product successfully solves the above three problems. Through the screening of enzymes and excipients with high stability, the storage stability of this product is consistent with that of glycerin-containing products; By selecting the appropriate lyophilized excipients and matching the lyophilization process, the yield and stability of the lyophilized products are improved, so that the lyophilized products can be stored at room temperature for more than one year. In addition, this product offers the advantages of conventional glycerol reagents, including good multiplex amplification performance, high specificity, and high sensitivity. The dUTP\/UDG anti-contamination system is incorporated into the reagents, which can be used at room temperature to eliminate the influence of amplification product contamination on qPCR results and ensure the accuracy of results.\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003eProbe gene expression analysis\u003cbr\u003eProbe Low-copy gene detection\u003cbr\u003eProbe microarray validation\u003cbr\u003eProbe gene knockdown validation\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003eOne-step RT-qPCR\u003cbr\u003eThis kit is suitable for fluorescence quantification by probe method\u003cbr\u003eThis kit is compatible with many real-time systems\u003cbr\u003eHot-start technology brings high specificity and reproducible amplification\u003cbr\u003edUTP\/UDG system, effectively prevent PCR product contamination\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"V5013-A\/200 rxns","offer_id":41401654280326,"sku":"V5013-A","price":338.0,"currency_code":"USD","in_stock":true},{"title":"V5013-B\/1000 rxns","offer_id":41401654313094,"sku":"V5013-B","price":1185.0,"currency_code":"USD","in_stock":true},{"title":"V5013-C\/5000 rxns","offer_id":41401654345862,"sku":"V5013-C","price":5538.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/V5013-1_2c7a36f9-efa0-480c-ad1e-801b89f216b3.jpg?v=1729499783"},{"product_id":"power-green-one-step-rt-qpcr-kit","title":"Power Green One-step RT-qPCR Kit","description":"\u003cp\u003ePower Green One-step RT-qPCR Kit is a one-step reverse transcriptation-fluorescence quantitative PCR kit. This product contains reverse transcriptase, Taq DNA polymerase, dNTPs, SYBR Green I dye and other reagents required for reverse transcription and qPCR reaction. By adding only RNA (such as viral RNA) and primers, reverse transcription and dye qPCR can be performed in the same reaction tube. This product reduces the steps of experimental operation, not only improves the efficiency of detection, but also reduces the risk of contamination. Kits are available in convenient Master Mix form. Power Green 1-step Enzyme Mix contains heat-resistant reverse transcriptase and antibody modified hotstart DNA polymerase, which can maintain stable reverse transcriptase activity at 55¡ãC to ensure high reverse transcriptase efficiency. hotstart Taq combined with optimized buffering system ensures high sensitivity and specificity of Power Green One-step RT-qPCR Kit. 2X Power Green 1-step Reaction Mix contains an optimized buffer system and SYBR Green I dye for SYBR\/FAM channel detection.\u003c\/p\u003e\n\n\u003cp\u003eApplication\u003cbr\u003e\nSYBR green I dye-based One-step RT-qPCR\u003c\/p\u003e\n\n\u003cp\u003eFeatures\u003cbr\u003e\nAll-in-one master mix, easy to use and saving time\u003cbr\u003e\nheat-resistant reverse transcriptase and antibody modified hotstart DNA polymerase\u003cbr\u003e\nHigh efficiency of reverse transcription and qPCR\u003cbr\u003e\nhigh sensitivity and specificity\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"V6001-A\/200 rxns","offer_id":41401654378630,"sku":"V6001-A","price":285.0,"currency_code":"USD","in_stock":true},{"title":"V6001-B\/5000 rxns","offer_id":41401654411398,"sku":"V6001-B","price":6425.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/V6001-1_0905881a-6d43-439f-96ee-5df246180a8a.jpg?v=1729499786"},{"product_id":"rt-pcr-kit","title":"RT-PCR Kit","description":"\u003cp\u003eRT-PCR Kit is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA. The RT-PCR Kit for RT-PCR delivers increased cDNA yields, high sensitivity, and full-length transcripts in a convenient format. You get all of the components you need for successful first-strand cDNA synthesis, saving your time and ensuring your success with every experiment. The kit Reverse Transcriptase is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability. The enzyme is used to synthesize cDNA at a temperature range of 42-55¡ãC, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases.\u003c\/p\u003e\n\n\u003cp\u003eApplication\u003cbr\u003e\nFirst strand cDNA synthesis for RT-PCR\u003cbr\u003e\nConstruction of cDNA libraries\u003cbr\u003e\nOne-step RT-PCR\u003cbr\u003e\nPrimer extension\u003c\/p\u003e\n\n\u003cp\u003eFeatures\u003cbr\u003e\nEach component individually packaged, flexible dosage\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"R1011\/20 rxns","offer_id":41401713590406,"sku":"R1011","price":60.0,"currency_code":"USD","in_stock":true},{"title":"R1012\/100 rxns","offer_id":41401713623174,"sku":"R1012","price":285.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_4649c787-5da2-45b4-8e86-499ab660134b.jpg?v=1745743852"},{"product_id":"rt-pcr-mix-for-qpcr","title":"RT-PCR Mix for qPCR","description":"\u003cp\u003eRT-PCR Mix for qPCR is a premixed reverse transcription reaction (RT-PCR) kit, easy to operate, can quickly and efficiently complete the reverse transcription reaction. RT-PCR Enzyme Mix contains highly efficient reverse transcriptase and heat labile DNase, which can complete genomic DNA clearance and RT-PCR of RNA in one step. 5X RT-PCR Buffer contains other components required for RT-PCR, without the need for multiple addition of components step by step, thus reducing the risk of sample contamination. At the same time, the kit is equipped with Control Mix for a negative control reaction to test for genomic DNA residue in the RNA template. The reverse transcription products of this kit have good compatibility for real-time PCR (qPCR) reaction by dye method and probe method, and can be used for accurate gene expression analysis.\u003c\/p\u003e\n\n\u003cp\u003eApplication\u003cbr\u003e\nFirst strand cDNA synthesis.\u003c\/p\u003e\n\n\u003cp\u003eFeatures\u003cbr\u003e\nBuffer mix + enzyme mix, easy to use\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"R1031\/100 rxns","offer_id":41401713655942,"sku":"R1031","price":131.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/R1031-1_77290c6f-603a-4338-b223-2e5db91abf3e.jpg?v=1729503202"},{"product_id":"m-mlv-reverse-transcriptase","title":"M-MLV Reverse Transcriptase","description":"\u003cp\u003eMoloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) uses single-stranded RNA or DNA in the presence of a primer to synthesize a complementary DNA strand. This enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. The enzyme is used to synthesize first-strand cDNA up to 5 kb.\u003cbr\u003eConcentration: 200U\/μl\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003e1st strand cDNA synthesis,especially for two-step RT-PCR\/qPCR\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003eThe optimum activity temperature is 37-42\u003cspan\u003e℃\u003c\/span\u003e\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"R1041\/5,000U","offer_id":41401713721478,"sku":"R1041","price":45.0,"currency_code":"USD","in_stock":true},{"title":"R1042\/10,000U","offer_id":41401713754246,"sku":"R1042","price":82.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/R1041-1_77de3c2f-8587-463d-8e11-90fdfd83d416.jpg?v=1729503206"},{"product_id":"powerscript-rt-supermix","title":"PowerScript RT SuperMix","description":"\u003cp\u003ePowerScript RT SuperMix is an optimized 5X reverse transcription mixture that contains all the ingredients required for first strand cDNA synthesis in a two-step RT-qPCR workflow. It contains thermostable reverse transcriptase, which supports cDNA synthesis at high temperatures. It also contains murine-derived RNase inhibitors to protect the template RNA from degradation. The mixture contains random haxmers and Oligo dT primers, which can fully perform reverse transcription of various kinds of RNA. This product can reverse transcribe as low as a single-copy of RNA, and the resulting cDNA product is an ideal template for qPCR detection, ensuring the sensitivity and accuracy of detection.\u003c\/p\u003e\n\n\u003cp\u003eApplication\u003cbr\u003e\nFirst strand cDNA synthesis.\u003c\/p\u003e\n\n\u003cp\u003eFeatures\u003cbr\u003e\nAll-in-one master mix, easy to use\u003cbr\u003e\nHigh efficiency of reverse transcription\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"R1081\/100 rxns","offer_id":41401713852550,"sku":"R1081","price":110.0,"currency_code":"USD","in_stock":true},{"title":"R1082\/500 rxns","offer_id":41401713885318,"sku":"R1082","price":480.0,"currency_code":"USD","in_stock":true},{"title":"R1083\/2500 rxns","offer_id":41401713918086,"sku":"R1083","price":1800.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_4649c787-5da2-45b4-8e86-499ab660134b.jpg?v=1745743852"},{"product_id":"rnasin","title":"RNasin","description":"\u003cp\u003eRNasin is a ribonuclease inhibitor purified from human placenta. RNasin reduces the activity of RNase A-type enzymes in a variety of organisms.\u003cbr\u003eConcentration: 40U\/\u003cspan\u003eμ\u003c\/span\u003el\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003eInhibition of RNase activity in cDNA synthesis and RT-PCR\/qPCR\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003eRecombination of human source\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"1,000U","offer_id":41401713950854,"sku":"R2011","price":44.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/R2011_Rnasin_package_82df645a-9a98-485b-8a3c-800762546866.jpg?v=1729503212"},{"product_id":"oligo-dt15-primer","title":"Oligo d(T)15 Primer","description":"\u003cp\u003eOligo d(T)15 Primer is a synthetic single-stranded 15-mer oligonucleotide with 5'- and 3'-hydroxyl ends. This primer is supplied as an aqueous solution and can be used for first strand cDNA synthesis.\u003cbr\u003e\nConcentration: 50¦ÌM\u003c\/p\u003e\n\n\u003cp\u003eApplication\u003cbr\u003e\nFirst strand cDNA synthesis.\u003c\/p\u003e\n\n\u003cp\u003eFeatures\u003cbr\u003e\nHigh purity\u003cbr\u003e\nuses all RNA as template for reverse transcription\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"20 μL","offer_id":41401713983622,"sku":"R2021","price":4.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/R2021_Ologo_Primer_50974d60-7f8b-4680-a4c6-522804f9134f.jpg?v=1729503215"},{"product_id":"random-primer","title":"Random Primer","description":"\u003cp\u003eThis primer is a mixture of single-stranded random hexanucleotides with 5'- and 3'-hydroxyl ends. The primer is supplied as an aqueous solution and can be used for first strand cDNA synthesis.\u003cbr\u003eConcentration: 50\u003cspan\u003eμ\u003c\/span\u003eM\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003eFirst strand cDNA synthesis.\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003eHigh purity\u003cbr\u003eonly uses mRNA as template for reverse transcription\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"20 μL","offer_id":41401714016390,"sku":"R2031","price":4.4,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/R2031_Random_hexamer_primer_package_318cf798-6161-41b9-9ba6-e30d867892b0.jpg?v=1729503218"},{"product_id":"gold-reverse-transcriptase","title":"Gold Reverse Transcriptase","description":"\u003cp\u003eGold Reverse Transcriptase is a new reverse transcriptase obtained by in vitro molecular evolution based on M-MLV (RNase H-) Reverse Transcriptase. The first strand of cDNA can be synthesized at 37~55\u003cspan\u003e℃\u003c\/span\u003e. Gold Reverse Transcriptase has further significantly improved sensitivity, specificity, thermal stability and half-life, which is very suitable for reverse transcription of RNA templates with complex secondary structure. Gold Reverse Transcriptase has stronger polymerization and extension ability, which can be used for the synthesis of long cDNA and the construction of high proportion of full-length cDNA library.\u003cbr\u003eConcentration: 200U\/\u003cspan\u003eμ\u003c\/span\u003el\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003e1st strand cDNA synthesis,especially for one-step RT-PCR\/qPCR\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003eThe optimum activity temperature is 50-55\u003cspan\u003e℃\u003c\/span\u003e\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"R3001\/2,000U","offer_id":41401714081926,"sku":"R3001","price":15.0,"currency_code":"USD","in_stock":true},{"title":"R3002\/10,000U","offer_id":41401714114694,"sku":"R3002","price":60.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/R3001-1_9d4e7ac5-93ab-487b-995a-6128b27bbfff.jpg?v=1729503223"},{"product_id":"rnase-inhibitor-murine","title":"RNase Inhibitor (Murine)","description":"\u003cp\u003eRNase Inhibitor (Murine) is a recombinant murine source RNase inhibitor expressed in the soluble form in E. coli. It can inhibit a wide range of RNases (RNase A, B, C). It has been tested by RT-PCR, RT-qPCR and is compatible with various reverse transcriptase and DNA polymerases. Compared with human source RNase inhibitors, murine source RNase inhibitors do not contain two of the very oxidation-sensitive cysteines of human proteins, so they have higher antioxidant activity and are more suitable for experiments with high DTT sensitivity, such as qPCR.\u003cbr\u003eConcentration: 40U\/\u003cspan\u003eμ\u003c\/span\u003el\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003eInhibition of RNase activity, more suitable for the one-step RT-PCR\/qPCR\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003eRecombination of murine source\u003cbr\u003eIts activity is less dependent on DTT\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"20,000U","offer_id":41401714147462,"sku":"R4001","price":136.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/R4001-1_a9926928-df04-4966-bb1d-c8aea8797e8c.jpg?v=1729503226"},{"product_id":"power-reverse-transcriptase","title":"Power Reverse Transcriptase","description":"\u003cp\u003ePower Reverse Transcriptase is a super thermostable reverse transcriptase derived from the Moloney murine leukemia virus (M-MLV) through genetic mutation screening. It synthesizes the first-strand cDNA at 42°C-55°C and withstands 65°C, making it ideal for high-temperature reverse transcription. The enzyme lacks RNase H activity and has high tolerance to common reverse transcriptase inhibitors. It is capable of reverse transcribing RNA with complex secondary structures, synthesizing long cDNA, and constructing full-length cDNA libraries with high proportion.\u003cbr\u003eConcentration: 200U\/\u003cspan\u003eμ\u003c\/span\u003el\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003cbr\u003e1st strand cDNA synthesis,especially for one-step RT-PCR\/qPCR\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003cbr\u003eThe optimum activity temperature is 42-55\u003cspan\u003e℃\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eTolerant to high temperatures up to 65℃\u003c\/span\u003e\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"R3011\/2,000U","offer_id":41707464687750,"sku":"R3011","price":16.5,"currency_code":"USD","in_stock":true},{"title":"R3012\/10,000U","offer_id":41707464720518,"sku":"R3012","price":66.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/R3001-1_9d4e7ac5-93ab-487b-995a-6128b27bbfff.jpg?v=1729503223"},{"product_id":"mirna-1st-strand-cdna-synthesis-kit-by-stem-loop","title":"miRNA 1st Strand cDNA Synthesis Kit by stem-loop","description":"\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003emiRNA 1st Strand cDNA Synthesis Kit (by stem-loop) is a reverse transcription kit for miRNA using the stem-loop method, also known as a first-strand cDNA synthesis kit. This kit employs a heat-resistant reverse transcriptase, combined with an optimized buffer system, to ensure the continuous and stable production of specific miRNA reverse transcription products. Additionally, the kit includes a genomic DNA removal step, which can rapidly eliminate genomic DNA contamination, making the results of subsequent qPCR and other experiments more accurate.\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003eFor the first-strand cDNA synthesis of miRNA using the stem-loop method.\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"R1091\/50 rxns","offer_id":41707556864134,"sku":"R1091","price":95.0,"currency_code":"USD","in_stock":true},{"title":"R1092\/100 rxns","offer_id":41707574984838,"sku":"R1092","price":180.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/R1031-1_77290c6f-603a-4338-b223-2e5db91abf3e.jpg?v=1729503202"},{"product_id":"mirna-1st-strand-cdna-synthesis-kit-by-tailing-a","title":"miRNA 1st Strand cDNA Synthesis Kit by tailing A","description":"\u003cp class=\"MsoNormal\"\u003emiRNA 1st Strand cDNA Synthesis Kit (by tailing A) is a polyadenylation-based reverse transcription kit for miRNA, also known as a first-strand cDNA synthesis kit. This kit uses miRNA or total RNA as a template. First, under the action of Poly A polymerase (PAP), poly (A) tails are added to the 3' end of miRNA to complete the polyadenylation reaction. Subsequently, a reverse transcriptase specifically engineered for miRNA reverse transcription and a universal reverse transcription primer are used, along with an optimized buffer system, to perform a highly sensitive reverse transcription reaction. Both the polyadenylation and reverse transcription reactions are carried out in the same tube, making the process convenient, time-saving, and reducing the risk of contamination.\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003ePolyadenylation-based first-strand cDNA synthesis from total RNA or miRNA.\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"R1101\/50 rxns","offer_id":41707580031110,"sku":"R1101","price":275.0,"currency_code":"USD","in_stock":true},{"title":"R1102\/100 rxns","offer_id":41707580063878,"sku":"R1102","price":575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/R1031-1_77290c6f-603a-4338-b223-2e5db91abf3e.jpg?v=1729503202"},{"product_id":"rt-pcr-grade-water","title":"RT-PCR Grade Water","description":"\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003eRT-PCR Grade Water is nuclease-free ultrapure water prepared for RT-PCR reactions, free of nucleic acid contamination that may cause false-positive results in RT-PCR. The product requires no sterilization, and is ready to use upon opening.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cb\u003e\u003ci\u003e\u003cspan class=\"15\"\u003eApplication\u003c\/span\u003e\u003c\/i\u003e\u003c\/b\u003e\u003cb\u003e\u003ci\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/i\u003e\u003c\/b\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003eRT-PCR experiments, various PCR experiments.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cb\u003e\u003ci\u003e\u003cspan class=\"15\"\u003eQuality Control\u003c\/span\u003e\u003c\/i\u003e\u003c\/b\u003e\u003ci\u003e\u003cspan\u003e \u003c\/span\u003e\u003c\/i\u003e\u003ci\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/i\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003eQuality testing indicates no contamination of nonspecific endonucleases, exonucleases, or ribonucleases.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"R2061\/5 ml","offer_id":41750233219206,"sku":"R2061","price":10.0,"currency_code":"USD","in_stock":true},{"title":"R2062\/50 ml","offer_id":41750233251974,"sku":"R2062","price":80.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/P9022_c2a0f25e-4040-4107-bb94-26b13a1661b4.jpg?v=1745312746"},{"product_id":"gdslyo-gold-reverse-transcriptase","title":"GDSlyo Gold Reverse Transcriptase","description":"\u003cp\u003eGDSLyo Gold Reverse Transcriptase is a glycerol-free version of the Gold Reverse Transcriptase (#R3001), suitable for the preparation of lyophilized reagents. Gold Reverse Transcriptase is a heat-resistant reverse transcriptase engineered from M-MLV. It can synthesize the first-strand cDNA at 50°C-55°C, with higher thermal stability. The enzyme lacks RNase H activity and has high tolerance to common reverse transcriptase inhibitors. It is capable of reverse transcribing RNA with complex secondary structures, synthesizing long cDNA, and constructing full-length cDNA libraries with high proportion.\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003eUsed for the preparation of lyophilized reagents related to RNA reverse transcription, especially for one-step RT-qPCR reagents.\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003eThe optimum activity temperature is 50-55\u003cspan\u003e℃\u003c\/span\u003e\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"R3001L\/10000U","offer_id":42149619597446,"sku":"R3001L","price":108.0,"currency_code":"USD","in_stock":true},{"title":"R3002L\/40000U","offer_id":42149619630214,"sku":"R3002L","price":408.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/R3001-1_9d4e7ac5-93ab-487b-995a-6128b27bbfff.jpg?v=1729503223"},{"product_id":"gdslyo-power-reverse-transcriptase","title":"GDSlyo Power Reverse Transcriptase","description":"\u003cp\u003eGDSLyo Power Reverse Transcriptase is a glycerol-free version of the Power Reverse Transcriptase (#R3011), suitable for the preparation of lyophilized reagents. Power Reverse Transcriptase is a super thermostable reverse transcriptase derived from the Moloney murine leukemia virus (M-MLV) through genetic mutation screening. It synthesizes the first-strand cDNA at 42°C-55°C and withstands 65°C, making it ideal for high-temperature reverse transcription. The enzyme lacks RNase H activity and has high tolerance to common reverse transcriptase inhibitors. It is capable of reverse transcribing RNA with complex secondary structures, synthesizing long cDNA, and constructing full-length cDNA libraries with high proportion.\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003eUsed for the preparation of lyophilized reagents related to RNA reverse transcription, especially for one-step RT-qPCR reagents.\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003eThe optimum activity temperature is 42-55℃\u003cbr\u003eTolerant to high temperatures up to 65℃\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"R3011L\/2000U","offer_id":42150008094854,"sku":"R3011L","price":29.0,"currency_code":"USD","in_stock":true},{"title":"R3012L\/10000U","offer_id":42150008127622,"sku":"R3012L","price":118.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/R3001-1_9d4e7ac5-93ab-487b-995a-6128b27bbfff.jpg?v=1729503223"},{"product_id":"maxscript-reverse-transcriptase","title":"MaxScript Reverse Transcriptase","description":"\u003cp\u003e\u003cstrong\u003eMaxScript Reverse Transcriptase\u003c\/strong\u003e is a heat-resistant reverse transcriptase engineered from M-MLV (RNase H-). MaxScript Reverse Transcriptase remains stable during prolonged reactions at 55°C, making it highly suitable for reverse transcription of RNA templates with complex secondary structures. This enzyme exhibits strong template affinity, allowing the synthesis of cDNA up to 20 kb in length. It is highly resistant to transcription inhibitors such as polysaccharides and polyphenols, making it ideal for synthesizing full-length cDNA.\u003cbr\u003eConcentration: 200U\/\u003cspan\u003eμ\u003c\/span\u003el\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003cbr\u003e1st strand cDNA synthesis,especially for one-step RT-PCR\/qPCR\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003cbr\u003eThe optimum activity temperature is 50-55\u003cspan\u003e℃\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"R3021\/2,000U","offer_id":42150469238918,"sku":"R3021","price":22.5,"currency_code":"USD","in_stock":true},{"title":"R3022\/10,000U","offer_id":42150469271686,"sku":"R3022","price":82.5,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/R3001-1_9d4e7ac5-93ab-487b-995a-6128b27bbfff.jpg?v=1729503223"},{"product_id":"direct-one-step-probe-rt-qpcr-kit","title":"Direct One-step Probe RT-qPCR Kit","description":"\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eDirect One-step Probe RT-qPCR Kit is a nucleic acid extraction-free, one-step probe-based RT-qPCR master mix. The Direct One-step Enzyme Mix contains optimized reverse transcriptase, antibody-modified hot-start Taq, and temperature-sensitive UDG enzyme, which eliminates DNA contamination at room temperature and is inactivated during the reverse transcription step. This product exhibits extremely high tolerance to inhibitors and can be used directly for target gene detection in various blood samples, swabs, and saliva. It is compatible with multiplex fluorescent PCR systems, supports fast protocols, and is suitable for pathogen detection, gene expression analysis, and other research and IVD development applications.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003cbr\u003eIVD product development for direct amplification of RNA virus samples and tumor samples.\u003cbr\u003eOne-step RT-qPCR experiments for multiple RNA targets.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eNo nucleic acid extraction required\u003c\/strong\u003e: Directly compatible with crude samples (e.g., whole blood, serum, saliva).\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eHigh inhibitor tolerance\u003c\/strong\u003e: Robust performance in complex sample matrices.\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eSupports fast cycling protocols\u003c\/strong\u003e: Ideal for rapid pathogen detection.\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eRoom-temperature UDG treatment\u003c\/strong\u003e: Prevents DNA contamination effectively.\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eSuitable for IVD development and research\u003c\/strong\u003e: Especially for RNA virus detection and direct amplification from tumor samples.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e","brand":"GDSBio","offers":[{"title":"V5014-A\/1000rxns (50-μl rxn)","offer_id":42412453200006,"sku":"V5014-A","price":3970.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/1-step3en.jpg?v=1756779152"},{"product_id":"one-step-probe-rt-qpcr-kit-v4","title":"One-step Probe RT-qPCR Kit V4","description":"\u003cp\u003eOne-step Probe RT-qPCR Kit V4 is a high-performance one-step probe-based reverse transcription quantitative PCR (RT-qPCR) master mix, consisting of two components: an enzyme mix and a reaction buffer. This product employs an optimized combination of antibody-modified hot-start Taq DNA polymerase and highly thermostable Power Reverse Transcriptase, coupled with a deeply optimized reaction buffer system, to achieve highly specific and sensitive detection results. The product offers excellent stability at ambient temperature, facilitating transportation and storage, and significantly improving user convenience. The dUTP\/temperature-sensitive UDG contamination prevention system effectively prevents false positives caused by PCR product carryover and aerosol contamination, ensuring the accuracy and reliability of results. It is suitable for developing various high-sensitivity RNA detection kits.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003cbr\u003eRNA virus \u0026amp; pathogen detection – e.g., SARS-CoV-2, HIV, influenza, dengue, HCV\u003cbr\u003eGene expression quantification – from low-abundance or precious RNA samples\u003cbr\u003eMultiplex target detection – simultaneous detection of 4–6 targets in one tube\u003cbr\u003eDirect amplification (no RNA extraction) – using crude lysates from swabs, blood, tissues\u003cbr\u003eLow-abundance \u0026amp; high-GC content RNA templates – high sensitivity down to a few copies or 0.1 pg total RNA\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eHigh-performance enzyme mix\u003c\/strong\u003e: Antibody-modified Taq and stable reverse transcriptase ensure high specificity and sensitivity.\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eOptimized buffer system\u003c\/strong\u003e: Enhances reaction efficiency and storage stability.\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003edUTP\/UDG anti-contamination system\u003c\/strong\u003e: Effectively prevents false positives from amplicon carryover.\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eBroad applicability\u003c\/strong\u003e: Suitable for multiplex detection and high-sensitivity RNA assay development.\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eExcellent amplification efficiency\u003c\/strong\u003e: Supports long amplicons (\u0026gt;200 bp) and low-efficiency primers; compatible with three-step cycling.\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/20260127172412.png?v=1769678791\" alt=\"\"\u003e\u003c\/p\u003e","brand":"GDSBio","offers":[{"title":"V5015-A\/1000rxns (50-μl rxn)","offer_id":42412469584006,"sku":"V5015-A","price":2650.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/1-step3en.jpg?v=1756779152"},{"product_id":"gdslyo-one-step-probe-rt-qpcr-kit-v2","title":"GDSlyo One-step Probe RT-qPCR Kit V2","description":"\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eGDSlyo One-step Probe RT-qPCR Kit V2 is a one-step probe-based RT-qPCR reagent meticulously developed for the lyophilization process. This product utilizes an optimized combination of a thermostable Power \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003eR\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eeverse \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003eT\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eranscriptase, engineered to remove RNase H activity, and a high-specificity hot-start DNA polymerase, significantly enhancing reverse transcription efficiency and PCR amplification performance. It delivers excellent standard curves over a wide linear range, making it particularly suitable for highly sensitive detection of low-concentration RNA templates. The reagent is equipped with a rigorously optimized qPCR-specific buffer system and a dUTP\/temperature-sensitive UDG contamination prevention system, effectively preventing false positives caused by aerosols and product carryover, ensuring accurate and reproducible results. The lyophilized product has a full form and excellent stability, suitable for storage and transportation at ambient temperature and various molecular testing scenarios.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003cbr\u003ePreparation of lyophilized RT-qPCR reagents.\u003cbr\u003eIVD product development for RNA detection (e.g., virus samples).\u003cbr\u003eOne-step RT-qPCR experiments for multiple RNA targets.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eOptimized for lyophilization\u003c\/strong\u003e: Includes lyoprotectant to maintain enzyme activity during freeze-drying.\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eHigh sensitivity and stability\u003c\/strong\u003e: Ideal for low-concentration RNA templates with a wide linear range.\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eAnti-contamination system\u003c\/strong\u003e: dUTP\/UDG prevents false positives from contamination.\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eSuitable for IVD development\u003c\/strong\u003e: Ideal for viral RNA detection and multiplex RT-qPCR.\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eFlexible manufacturing process\u003c\/strong\u003e: Supports customizable lyophilization protocols.\u003c\/p\u003e","brand":"GDSBio","offers":[{"title":"V5016-A\/2000rxns (25-μl rxn)","offer_id":42412475744390,"sku":"V5016-A","price":2650.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/1-step3en.jpg?v=1756779152"},{"product_id":"gdslyo-fast-one-step-probe-rt-qpcr-kit","title":"GDSlyo Fast One-step Probe RT-qPCR Kit","description":"\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eGDSlyo Fast One-step Probe RT-qPCR Kit is a one-step probe-based RT-qPCR reagent optimized for the lyophilization process. It employs an optimized combination of a genetically engineered fast thermostable reverse transcriptase and a high-fidelity hot-start DNA polymerase, coupled with a highly efficient reaction buffer system, enabling complete amplification in just 20-40 minutes, significantly improving detection throughput. The included thermostable reverse transcriptase is engineered to remove RNase H activity, offering higher cDNA synthesis efficiency and amplification sensitivity, making it particularly suitable for the stable detection of low-concentration RNA templates.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThis kit produces excellent standard curves over a wide linear range, ensuring accurate and reliable quantification. It is also compatible with various fast PCR instruments and rapid amplification programs, meeting the needs of high-throughput and rapid testing applications. The built-in optimized qPCR buffer and dUTP\/temperature-sensitive UDG contamination prevention system effectively eliminate false positives caused by aerosols and product carryover. The lyophilized product has good formability and excellent stability, suitable for ambient temperature storage and transportation and various molecular diagnostic scenarios.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003cbr\u003ePreparation of lyophilized RT-qPCR reagents.\u003cbr\u003eIVD product development for RNA detection (e.g., virus samples).\u003cbr\u003eOne-step RT-qPCR experiments for multiple RNA targets.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eFast lyophilized RT-qPCR kit\u003c\/strong\u003e: Designed for rapid detection with short RT and amplification times.\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eEffective lyoprotection\u003c\/strong\u003e: Maintains enzyme activity and stability after lyophilization.\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eHigh inhibitor tolerance\u003c\/strong\u003e: Suitable for direct amplification from crude samples.\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eDual cycling protocols\u003c\/strong\u003e: Offers both standard and fast programs for flexibility.\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eIdeal for IVD and POCT\u003c\/strong\u003e: Suitable for developing rapid diagnostic tests and point-of-care applications.\u003c\/p\u003e","brand":"GDSBio","offers":[{"title":"V5017-A\/2000rxns (25-μl rxn)","offer_id":42412479381638,"sku":"V5017-A","price":2910.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/1-step3en.jpg?v=1756779152"},{"product_id":"gdslyo-one-step-probe-rt-qpcr-kit-v2-naf","title":"GDSlyo One-step Probe RT-qPCR Kit V2-NAF","description":"\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eGDSlyo One-step Probe RT-qPCR Kit V2\u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003e-NAF\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003e is a one-step probe-based RT-qPCR reagent meticulously developed for the lyophilization process. This product utilizes an optimized combination of a thermostable Power \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003eR\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eeverse \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003eT\u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eranscriptase, engineered to remove RNase H activity, and a high-specificity hot-start DNA polymerase, significantly enhancing reverse transcription efficiency and PCR amplification performance. It delivers excellent standard curves over a wide linear range, making it particularly suitable for highly sensitive detection of low-concentration RNA templates. The reagent is equipped with a rigorously optimized qPCR-specific buffer system and a dUTP\/temperature-sensitive UDG contamination prevention system, effectively preventing false positives caused by aerosols and product carryover, ensuring accurate and reproducible results. The lyophilized product has a full form and excellent stability, suitable for storage and transportation at ambient temperature and various molecular testing scenarios.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThis product is processed with special technology to ensure it is free of exogenous DNA residues, meeting the high-standard application requirements of biopharmaceuticals, cell therapy, and vaccine quality control.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003cbr\u003ePreparation of lyophilized RT-qPCR reagents.\u003cbr\u003eIVD product development for RNA detection (e.g., virus samples).\u003cbr\u003eBiopharmaceutical quality control, cell therapy product testing.\u003cbr\u003eOne-step RT-qPCR experiments for multiple RNA targets.\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e","brand":"GDSBio","offers":[{"title":"V5016F-A\/2000rxns","offer_id":42444045090950,"sku":"V5016F-A","price":9999.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/1-step3en.jpg?v=1756779152"},{"product_id":"gdslyo-fast-direct-one-step-probe-rt-qpcr-kit-naf","title":"GDSlyo Fast Direct One-step Probe RT-qPCR Kit (NAF)","description":"\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eGDSlyo Fast Direct One-Step Probe RT-qPCR Kit \u003c\/span\u003e\u003cspan\u003e\u003cspan style=\"font-family: Arial;\"\u003e(NAF) \u003c\/span\u003e\u003c\/span\u003e\u003cspan\u003eis a one-step probe-based reverse transcription quantitative PCR reagent specifically optimized for lyophilization processes, eliminating the need for nucleic acid extraction. This product utilizes an optimized combination of genetically engineered fast thermostable reverse transcriptase and hot-start high-fidelity DNA polymerase, coupled with a highly efficient reaction buffer system, enabling complete amplification within 20–40 minutes, significantly improving detection efficiency. It exhibits extremely high tolerance to inhibitors and can be directly used for target gene detection in various blood samples, swabs, saliva, and other sample types. It is also compatible with various fast PCR instruments and rapid amplification programs, meeting the demands of high-throughput and rapid testing applications. The reagent features an optimized qPCR buffer and a dUTP\/thermolabile UDG anti-contamination system, effectively preventing false positives caused by aerosol contamination and amplicon carryover. The product demonstrates excellent lyophilization formability and stability, making it suitable for room temperature storage and transportation, as well as various molecular diagnostic scenarios.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eThis product undergoes special processing to ensure the absence of exogenous DNA contamination, complying with the high-standard application requirements of biopharmaceuticals, cell therapy, vaccine quality control, and more.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003cbr\u003ePreparation of lyophilized RT-qPCR reagents.\u003cbr\u003eIVD product development for RNA detection (e.g., virus samples).\u003cbr\u003eOne-step RT-qPCR experiments for multiple RNA targets.\u003c\/span\u003e\u003c\/p\u003e","brand":"GDSBio","offers":[{"title":"V5018F-A\/2000rxns","offer_id":42444051972230,"sku":"V5018F-A","price":9999.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/1-step3en.jpg?v=1756779152"},{"product_id":"dspath-5x-plus-one-step-multiplex-master-mix","title":"DSPath 5X Plus One-Step Multiplex Master Mix","description":"\u003cp\u003eDSPath™ 5X Plus One-Step Multiplex Master Mix is a one-tube, ultra-concentrated master mix specially designed for ultra-sensitive multiplex quantitative PCR, completing the reverse transcription and qPCR detection of RNA samples in a single step. On the basis of inheriting the excellent multiplex amplification buffer system and heat-labile UDG anti-contamination system of the original V5005, the reagent concentration has been upgraded to a 5X ultra-concentrated state, allowing for a larger template input volume and increasing the detection rate of low-copy samples. The formulation of this product has been deeply optimized to possess fast amplification performance and superior sample tolerance. It supports pre-mixing primers and probes into the 5X master mix to prepare a working solution, which can be stably stored at 4°C\/-20°C for a long time, achieving minimal manual operations and high-throughput automated liquid handling.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003cbr\u003ePathogen detection\u003cbr\u003eLow-copy \/ low-abundance target detection\u003cbr\u003eSimultaneous detection of multiple targets\u003cbr\u003eHigh-throughput automation platforms\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003cbr\u003eUltra‑high sensitivity\u003cbr\u003eRapid amplification\u003cbr\u003eStrong sample tolerance\u003cbr\u003eConvenient workflow\u003cbr\u003eThermolabile UDG carryover prevention system\u003cbr\u003e\u003c\/p\u003e","brand":"GDSBio","offers":[{"title":"V5005P\/200rxns","offer_id":44116730773638,"sku":"V5005P","price":9999.0,"currency_code":"USD","in_stock":true},{"title":"V5006P\/5000rxns","offer_id":44116730806406,"sku":"V5006P","price":9999.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/P2701_PS.jpg?v=1778571865"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/collections\/V5011-2.jpg?v=1729824298","url":"https:\/\/www.gdsbio.com\/collections\/rt-rt-pcr-qpcr.oembed","provider":"GDSBio","version":"1.0","type":"link"}