{"title":"Tool Enzyme","description":"\u003cp\u003eGDSBio provides a complete range of PCR-based molecular diagnostic raw materials and solutions.\u003c\/p\u003e","products":[{"product_id":"dszonase-endonuclease","title":"Dszonase Endonuclease","description":"\u003cp\u003eDszonase Endonuclease is a non-specific endonuclease derived from Serratia Marcescen that fully digesters all forms of DNA and RNA (single, double, linear and cyclic) 5 '-monophosphate terminal oligonucleotides with a growth of 3 to 5 bases. Dszonase Endonuclease is a purified recombinant protein expressed in E. coli that does not have any proteolytic activity and is used for the removal of nucleic acids from recombinant proteins or other applications that require complete digestion of nucleic acids.\u003c\/p\u003e\n\n\u003cp\u003eScope of Application \u003cbr\u003e\nReduces the viscosity of the protein extract\u003cbr\u003e\nSample preparation for 2D gel electrophoresis\u003cbr\u003e\nRemove nucleic acid contaminants from recombinant protein preparations\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"E1011-A\/5 KU","offer_id":41510120358022,"sku":"E1011-A","price":15.5,"currency_code":"USD","in_stock":true},{"title":"E1011-B\/25 KU","offer_id":41510120390790,"sku":"E1011-B","price":69.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_5c94cf03-c153-46f0-a793-3b3e562db66f.jpg?v=1736413203"},{"product_id":"taq-dna-ligase","title":"Taq DNA Ligase","description":"\u003cp\u003e40U\/µL\u003c\/p\u003e\n\u003cp\u003eTaq DNA Ligase is a heat-resistant ligase that catalyzes the formation of phosphodiester bonds at the 5' -phosphate and 3' -hydroxyl ends of two adjacent DNA strands. This reaction can occur only when the two oligonucleotide chains are perfectly paired with the complementary target DNA and there is no gap between the two oligonucleotide chains. Therefore, it can be used to detect single nucleotide variants. Taq DNA ligase uses NAD as a cofactor. Taq DNA ligase was active in the range of 37-75\u003cspan\u003e°C\u003c\/span\u003e.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eSource\u003cbr\u003eRecombinant E. coli strain containing ligase gene cloned from Thermus aquaticus HB8.\u003c\/p\u003e\n\u003cp\u003eUnit Definition\u003cbr\u003e\u003cspan\u003e1 unit is the amount of enzyme required to bind 50% of 1 µg BstEII enzyme to the 12-base pair sticky\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003eend of λ DNA for 15 minutes at 45 ° C at a total reaction volume of 50 µl.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eScope of Application \u003cbr\u003eUsing ligase chain reaction (LCR) and ligase detection reaction (LDR) to specifically detect alleles\u003cbr\u003eIncorporating phosphorylated oligonucleotides during primer extension amplification for mutation detection\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"E1012-A\/1,000 U","offer_id":41511816396934,"sku":"E1012-A","price":43.0,"currency_code":"USD","in_stock":true},{"title":"E1012-B\/2,000 U","offer_id":41511816429702,"sku":"E1012-B","price":73.0,"currency_code":"USD","in_stock":true},{"title":"E1012-C\/10,000 U","offer_id":41511816462470,"sku":"E1012-C","price":300.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_3520ec94-21d7-4c2d-a33b-15e45d369819.jpg?v=1736488982"},{"product_id":"thermosensitive-alkaline-phosphatase","title":"Thermosensitive Alkaline Phosphatase","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConcentration: 1 \u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eU\/µL\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThermosensitive Alkaline Phosphatase is a novel alkaline phosphatase that exhibits activity in commonly used restriction endonuclease buffers as well as PCR buffers. This enzyme catalyzes the release of 5' and 3' phosphate groups in DNA, RNA, and nucleotides. Additionally, it can remove phosphate groups from proteins. Thermosensitive Alkaline Phosphatase is capable of dephosphorylating all types of DNA termini within 10 minutes at 37°C. The enzyme is inactivated by heat at 75°C within 5 minutes. Consequently, there is no need to remove the alkaline phosphatase before ligation.\u003c\/p\u003e\n\u003cp\u003eSource\u003cbr\u003eRecombinant E. coli strain containing the alkaline phosphatase gene cloned from bacteria.\u003c\/p\u003e\n\u003cp\u003eUnit Definition\u003cbr\u003e\u003cspan\u003eOne unit is defined as the amount of enzyme required to dephosphorylate the 5'-ends of 1 μg of linearized pUC57 DNA in DSAP Buffer at 37°C within 10 minutes.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eScope of Application \u003cbr\u003e- Dephosphorylation of cloned vector DNA to prevent re-circularization during ligation.\u003cbr\u003e- Simultaneous enzymatic cleavage and dephosphorylation of vector DNA.\u003cbr\u003e- Purification of PCR products: nucleotide degradation of PCR products before sequencing.\u003cbr\u003e- Depehosphorylation of the 5'-ends of nucleic acids before T4 polynucleotide kinase labeling.\u003cbr\u003e- Other applications requiring dephosphorylation of DNA and RNA substrates.\u003cbr\u003e- Dephosphorylation of proteins.\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"E1013-A\/300 U","offer_id":41511816528006,"sku":"E1013-A","price":90.0,"currency_code":"USD","in_stock":true},{"title":"E1013-B\/1,000 U","offer_id":41511816560774,"sku":"E1013-B","price":152.0,"currency_code":"USD","in_stock":true},{"title":"E1013-C\/5,000 U","offer_id":41511816593542,"sku":"E1013-C","price":609.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_f02f49ec-7b3e-4ff3-9a57-c510dc23003f.jpg?v=1736488985"},{"product_id":"t4-dna-ligase","title":"T4 DNA Ligase","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConcentration: 5 Weiss U\/\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eT4 DNA Ligase catalyzes the formation of phosphodiester bonds between juxtaposed 5'-phosphate and 3'-hydroxyl termini in double-stranded DNA or RNA. This enzyme is capable of repairing single-strand nicks in double-stranded DNA, RNA, or DNA\/RNA hybrids, and can join DNA fragments with sticky or blunt ends, but it is inactive on single-stranded nucleic acids.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eT4 DNA Ligase requires ATP as a cofactor.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eSource\u003cbr\u003eRecombinant E. coli strain containing the gene 30 cloned from bacteriophage T4.\u003cbr\u003e\u003cbr\u003eUnit Definition\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAt 37\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e, 1 Weiss unit of enzyme catalyzes the conversion of 1 nmol of [\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e32\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ePPi] into an activated carbon adsorption state within 20 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEnzyme activity is determined in the following mixture: 66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e, 0.066 mM ATP, 10 mM DTT, 3.3 μM [\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e32\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eP]orthophosphate (PPi).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eOne Weiss unit is equivalent to approximately 200 cohesive end ligation units (CEU), which is equivalent to the number of HindIII digested DNA fragments that can be ligated within 30 minutes at 16\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e. λ DNA fragments.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eScope of Application \u003cbr\u003e- DNA fragments generated by cloning restriction enzymes\u003cbr\u003e- Cloned PCR products\u003cbr\u003e- Ligation of double-stranded oligonucleotides or adapters to DNA\u003cbr\u003e- Site-directed mutagenesis\u003cbr\u003e- Amplified fragment length polymorphism (AFLP)\u003cbr\u003e- Ligase-mediated RNA detection\u003cbr\u003e- Nick repair in double-stranded DNA, RNA, or DNA\/RNA hybrids\u003cbr\u003e- Self-circularization of linear DNA\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"200 U","offer_id":41511816724614,"sku":"E1014-A","price":56.0,"currency_code":"USD","in_stock":true},{"title":"1,000 U","offer_id":41511816757382,"sku":"E1014-B","price":134.0,"currency_code":"USD","in_stock":true},{"title":"5,000 U","offer_id":41511816790150,"sku":"E1014-C","price":531.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_43cbf9d6-9381-4783-8b9c-6e86e12092d7.jpg?v=1736488988"},{"product_id":"t4-dna-ligase-hc","title":"T4 DNA Ligase, HC","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConcentration: 30 Weiss U\/\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eT4 DNA Ligase catalyzes the formation of phosphodiester bonds between juxtaposed 5'-phosphate and 3'-hydroxyl termini in double-stranded DNA or RNA. This enzyme is capable of repairing single-strand nicks in double-stranded DNA, RNA, or DNA\/RNA hybrids, and can join DNA fragments with sticky or blunt ends, but it is inactive on single-stranded nucleic acids.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eT4 DNA Ligase requires ATP as a cofactor.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eSource\u003cbr\u003eRecombinant E. coli strain containing the gene 30 cloned from bacteriophage T4.\u003cbr\u003e\u003cbr\u003eUnit Definition\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAt 37\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e, 1 Weiss unit of enzyme catalyzes the conversion of 1 nmol of [\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e32\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ePPi] into an activated carbon adsorption state within 20 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEnzyme activity is determined in the following mixture: 66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e, 0.066 mM ATP, 10 mM DTT, 3.3 μM [\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e32\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eP]orthophosphate (PPi).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eOne Weiss unit is equivalent to approximately 200 cohesive end ligation units (CEU), which is equivalent to the number of HindIII digested DNA fragments that can be ligated within 30 minutes at 16\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e. λ DNA fragments.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eScope of Application \u003cbr\u003e- DNA fragments generated by cloning restriction enzymes\u003cbr\u003e- Cloned PCR products\u003cbr\u003e- Ligation of double-stranded oligonucleotides or adapters to DNA\u003cbr\u003e- Site-directed mutagenesis\u003cbr\u003e- Amplified fragment length polymorphism (AFLP)\u003cbr\u003e- Ligase-mediated RNA detection\u003cbr\u003e- Nick repair in double-stranded DNA, RNA, or DNA\/RNA hybrids\u003cbr\u003e- Self-circularization of linear DNA\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"E1014-D\/5,000 U","offer_id":41511818100870,"sku":"E1014-D","price":531.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_7397c561-b7ed-4025-aa7f-94d2a73bb38d.jpg?v=1736488992"},{"product_id":"t4-dna-ligase-lc","title":"T4 DNA Ligase, LC","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConcentration: 1 Weiss U\/\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eT4 DNA Ligase catalyzes the formation of phosphodiester bonds between juxtaposed 5'-phosphate and 3'-hydroxyl termini in double-stranded DNA or RNA. This enzyme is capable of repairing single-strand nicks in double-stranded DNA, RNA, or DNA\/RNA hybrids, and can join DNA fragments with sticky or blunt ends, but it is inactive on single-stranded nucleic acids.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eT4 DNA Ligase requires ATP as a cofactor.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eSource\u003cbr\u003eRecombinant E. coli strain containing the gene 30 cloned from bacteriophage T4.\u003cbr\u003e\u003cbr\u003eUnit Definition\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAt 37\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e, 1 Weiss unit of enzyme catalyzes the conversion of 1 nmol of [\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e32\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ePPi] into an activated carbon adsorption state within 20 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEnzyme activity is determined in the following mixture: 66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e, 0.066 mM ATP, 10 mM DTT, 3.3 μM [\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e32\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eP]orthophosphate (PPi).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eOne Weiss unit is equivalent to approximately 200 cohesive end ligation units (CEU), which is equivalent to the number of HindIII digested DNA fragments that can be ligated within 30 minutes at 16\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e. λ DNA fragments.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eScope of Application \u003cbr\u003e- DNA fragments generated by cloning restriction enzymes\u003cbr\u003e- Cloned PCR products\u003cbr\u003e- Ligation of double-stranded oligonucleotides or adapters to DNA\u003cbr\u003e- Site-directed mutagenesis\u003cbr\u003e- Amplified fragment length polymorphism (AFLP)\u003cbr\u003e- Ligase-mediated RNA detection\u003cbr\u003e- Nick repair in double-stranded DNA, RNA, or DNA\/RNA hybrids\u003cbr\u003e- Self-circularization of linear DNA\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"E1014-E\/1,000 U","offer_id":41511818133638,"sku":"E1014-E","price":137.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_7fe6f826-ee9b-4a06-932b-f9b950923ed8.jpg?v=1736488995"},{"product_id":"t4-rna-ligase","title":"T4 RNA Ligase","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConcentration: 10 U\/\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eT4 RNA Ligase catalyzes the ATP-dependent formation of phosphodiester bonds between 5'-phosphate groups and 3'-hydroxyl termini of oligonucleotides, single-stranded RNA, and DNA molecules both intermolecularly and intramolecularly.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eMinimum substrates: a nucleotide 3',5'-phosphate in intermolecular reactions and an 8-nucleotide oligonucleotide in intramolecular reactions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eSource\u003cbr\u003eRecombinant E. coli strain contains the gene 63 cloned from bacteriophage T4.\u003c\/p\u003e\n\u003cp\u003eUnit Definition\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA unit is defined as the amount of enzyme required to catalyze the conversion of 1 nmol of 5'-[\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e32\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eP]-(A)\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e12-18\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e to its acid-resistant form at 37°C within 30 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEnzyme activity is determined in the following mixture: 50 mM Tris-HCl (pH 7.5), 10 mM MgCl\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e, 10 mM DTT, 1 mM ATP, and 10 \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003eM 5'-[\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e32\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eP]-(A)\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e12-18\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e (10 µM at the 5'-terminus).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eScope of Application \u003cbr\u003e- RNA 3'-terminal labeling (using cytidine \u003cspan\u003e3',5'-di[α-\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e32\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eP]\u003c\/span\u003e phosphate)\u003cbr\u003e- RNA-to-RNA ligation\u003cbr\u003e- Synthesis of oligoribonucleotides and oligodeoxyribonucleotides\u003cbr\u003e- Specific modification of tRNA\u003cbr\u003e- Ligation of oligodeoxynucleotides to single-stranded cDNA for 5' RACE (Rapid Amplification of cDNA Ends)\u003cbr\u003e- Site-specific generation of PCR primer complexes\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"E1015-A\/1,000 U","offer_id":41511818166406,"sku":"E1015-A","price":143.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_bb37d985-398c-4170-b1c9-8741ee6ea60a.jpg?v=1736488998"},{"product_id":"rnase-h","title":"RNase H","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConcentration: 5 U\/\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eProduct Description \u003cbr\u003eRibonuclease H (RNase H) can specifically degrade the RNA strand within RNA-DNA hybrids. This enzyme does not hydrolyze the phosphodiester bonds in single-stranded or double-stranded DNA and RNA.\u003c\/p\u003e\n\u003cp\u003eSource\u003cbr\u003eE. coli strain MRE-600.\u003c\/p\u003e\n\u003cp\u003eUnit Definition\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA unit is defined as the amount of enzyme required to catalyze the formation of 1 nmol of acid-soluble products within 20 minutes at 37°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEnzyme activity is determined in the following mixture: 20 mM Tris-HCl (pH 7.8), 40 mM KCl, 8 mM MgCl\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e,1 mM DTT, 24 µM [\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eH]-poly(A)·poly(dT), 0.03 mg\/mL BSA, and 4% (v\/v) glycerol.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eScope of Application \u003cbr\u003e- Removal of mRNA before the synthesis of the second-strand cDNA\u003cbr\u003e- RT-PCR and qRT-PCR: Removal of RNA after the first-strand cDNA synthesis\u003cbr\u003e- Removal of poly(A) sequence from mRNA after hybridization with Oligo(dT)\u003cbr\u003e- Site-specific cleavage of RNA\u003cbr\u003e- Study of products from in vitro polyadenylation reactions\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"E1016-A\/100 U","offer_id":41511820165254,"sku":"E1016-A","price":34.0,"currency_code":"USD","in_stock":true},{"title":"E1016-B\/500 U","offer_id":41511820198022,"sku":"E1016-B","price":128.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_a798d2db-fb30-41ee-8438-2b25c10c5296.jpg?v=1736489001"},{"product_id":"s1-nuclease","title":"S1 Nuclease","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConcentration: 100 U\/\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eProduct Description \u003cbr\u003eS1 nuclease can degrade single-stranded nucleic acids, releasing 5'-phosphorylated mononucleotides or oligonucleotides. Its activity on DNA is five times that on RNA. S1 nuclease can also cleave double-stranded DNA (dsDNA) at single-stranded regions caused by nicks, gaps, mismatches, or loops. S1 nuclease exhibits 3'-phosphomonoesterase activity.\u003cbr\u003eThis enzyme is a glycoprotein with a carbohydrate content of 18%.\u003c\/p\u003e\n\u003cp\u003eSource\u003cbr\u003eAspergillus oryzae strain.\u003c\/p\u003e\n\u003cp\u003eUnit Definition\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA unit is defined as the amount of enzyme required to produce 1 \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003eg of acid-soluble deoxyribonucleotides in 1 minute at 37°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEnzyme activity is determined in the following mixture: 30 mM sodium acetate (pH 4.5), 50 mM NaCl, 0.1 mM ZnCl\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e, 5% (v\/v) glycerol, and 800 \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003eg\/mL heat-denatured calf thymus DNA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eScope of Application \u003cbr\u003e- Removal of single-stranded overhangs from DNA fragments\u003cbr\u003e- Localization of S1 transcripts\u003cbr\u003e- Cleavage of hairpin loops\u003cbr\u003e- In conjunction with Exonuclease III, creating unidirectional deletions within DNA fragments\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"E1017-A\/10,000 U","offer_id":41511820230790,"sku":"E1017-A","price":73.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_0eb42cba-2125-4e4c-a00f-d1dc411c16ac.jpg?v=1736489005"},{"product_id":"dnase-i-rnase-free-hc","title":"DNase I, RNase-Free, HC","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConcentration: 50 U\/\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eProduct Description \u003cbr\u003eDNase I (without RNase) is an endonuclease that can cleave both single-stranded and double-stranded DNA. It hydrolyzes phosphodiester bonds, producing single deoxyribonucleotides and oligodeoxyribonucleotides with 5'-phosphate groups and 3'-OH groups.\u003cbr\u003eThe activity of this enzyme is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions. In the presence of Mg2+, DNase I cuts each strand of dsDNA in a statistically random and independent manner. When Mn2+ is present, the enzyme almost cuts both DNA strands at the same site, resulting in DNA fragments with blunt ends or overhangs of one or a few nucleotides.\u003c\/p\u003e\n\u003cp\u003eSource\u003cbr\u003eRecombinant E. coli strain containing the cloned gene encoding bovine DNase I.\u003c\/p\u003e\n\u003cp\u003eUnit Definition\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA unit is defined as the amount of enzyme required to completely degrade 1 µg of plasmid DNA within 1 minute at 37°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEnzyme activity is determined in the following mixture: 10 mM Tris-HCl (pH 7.5 at 25°C), 2.5 mM MgCl\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e, 0.1 mM CaCl\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e, and 1 µg of pUC19 DNA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eOne unit of DNase I is equivalent to 0.3 Kunitz units.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003e- Recombinant enzyme\u003cbr\u003e- Purified from a non-animal host, with low levels of endogenous RNase\u003c\/p\u003e\n\u003cp\u003eScope of Application \u003cbr\u003e- For the preparation of DNA-free RNA.\u003cbr\u003e- To remove template DNA after in vitro transcription.\u003cbr\u003e- To prepare DNA-free RNA before RT-PCR and RT-qPCR.\u003cbr\u003e- In conjunction with DNA Polymerase I for DNA labeling through nick translation.\u003cbr\u003e- For the study of DNA-protein interactions using DNase I (RNase-free) footprinting.\u003cbr\u003e- To generate a library of randomly sheared DNA insert fragments. Reaction buffer containing Mn2+ is used.\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"1,000 U","offer_id":41511820263558,"sku":"E1018-A","price":98.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_55d87818-944f-4e6b-b991-27c5cdc62a64.jpg?v=1736489008"},{"product_id":"proteinase-k-pcr-grade","title":"Proteinase K (recombinant), PCR grade","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConcentration: \u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e≥\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e 600 U\/m\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eL (~20 mg\/mL)\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eProduct Description \u003cbr\u003eProteinase K is an endopeptidase capable of hydrolyzing peptide bonds at the carboxyl terminus of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K belongs to the family of serine proteases. This enzyme can minimally hydrolyze tetra-peptides.\u003c\/p\u003e\n\u003cp\u003eSource\u003cbr\u003eRecombinant Pichia pastoris strain containing a cloned gene from Tritirachium album.\u003c\/p\u003e\n\u003cp\u003eUnit Definition\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA unit is defined as the amount of enzyme required to release an amount of Folin-positive amino acids and peptides equivalent to 1 \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003emol of tyrosine from denatured hemoglobin in 1 minute at 37°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEnzyme activity is determined in the following mixture: 0.08M potassium phosphate (pH 7.5), 5M urea, 4mM NaCl, 3mM CaCl\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e, and 16.7 mg\/mL hemoglobin.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003e- Ready-to-use solution\u003cbr\u003e- Compatible with a wide range of reaction conditions\u003c\/p\u003e\n\u003cp\u003eScope of Application \u003cbr\u003e- Isolate genomic DNA from mouse tails.\u003cbr\u003e- Isolate genomic DNA from cultured cells.\u003cbr\u003e- Eliminate DNase and RNase during the isolation of DNA and RNA from tissues or cell lines.\u003cbr\u003e- Determine the location of enzymes.\u003cbr\u003e- Improve the cloning efficiency of PCR products.\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"E1019-A\/1 mL","offer_id":41511820361862,"sku":"E1019-A","price":55.0,"currency_code":"USD","in_stock":true},{"title":"E1019-B\/1 mL*5","offer_id":41511820394630,"sku":"E1019-B","price":250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_725f78e5-1ff3-45e0-bff2-0489a6d417ce.jpg?v=1736489012"},{"product_id":"t4-b-glucosyltransferase","title":"T4 β-Glucosyltransferase","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConcentration: 5 U\/\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eProduct Description \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eT4 β-glucosyltransferase (T4 BGT) is capable of transferring the glucosyl group from uridine diphosphate glucose (UDP-glucose) to the 5-hydroxymethylcytosine (5-hmC) residues within double-stranded DNA, resulting in the formation of β-glucosyl-5-hydroxymethylcytosine. This enzyme is formulated for rapid reaction times without compromising the efficiency of the reaction. It can glucosylate 5-hmC on 1 \u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eg of DNA in 15 minutes at 37°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eUnit Definition\u003cbr\u003e\u003cspan\u003eA unit is defined as the amount of enzyme required to protect 0.5 \u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eg of fully 5-hydroxymethylated 1095bp PCR fragment from digestion by MunI within 1 hour at 37°C in a 50 \u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL volume of the recommended reaction buffer.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003e- Specificity - Selectively transfers glucose to the hydroxymethyl group of 5-hmC.\u003cbr\u003e- Speed - Completes the glucosylation of 1 \u003cspan\u003eμ\u003c\/span\u003eg DNA in 15 minutes.\u003cbr\u003e- Convenience - Comes with optimized buffer and UDP-glucose included.\u003c\/p\u003e\n\u003cp\u003eScope of Application \u003cbr\u003e- Site-specific detection of 5-hmC.\u003cbr\u003e- Enrichment of DNA containing 5-hmC.\u003cbr\u003e- Labeling of 5-hmC residues using radioactive UDP-glucose donor.\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"E1020-A\/500 U","offer_id":41511820460166,"sku":"E1020-A","price":272.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_3115bf98-382f-47df-b0f6-ab880cc18ba6.jpg?v=1736489015"},{"product_id":"dna-polymerase-i","title":"DNA Polymerase I","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConcentration: 10 U\/\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eProduct Description \u003cbr\u003e\u003cspan\u003eThis product is a template-dependent DNA polymerase capable of accelerating the synthesis of DNA in the 5'\u003c\/span\u003e\u003cspan\u003e→\u003c\/span\u003e\u003cspan\u003e3' direction. It also possesses 3'\u003c\/span\u003e\u003cspan\u003e→\u003c\/span\u003e\u003cspan\u003e5' exonuclease (proofreading) activity, 5'\u003c\/span\u003e\u003cspan\u003e→\u003c\/span\u003e\u003cspan\u003e3' exonuclease activity, and RNase H activity.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eSource\u003cbr\u003eRecombinant E. coli strain containing the cloned gene polA from E. coli.\u003c\/p\u003e\n\u003cp\u003eUnit Definition\u003cbr\u003e\u003cspan\u003eA unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of deoxynucleotides into a polynucleotide substrate within 30 minutes at 37\u003c\/span\u003e\u003cspan\u003e°\u003c\/span\u003e\u003cspan\u003eC.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003e- Incorporation of modified nucleotides (such as biotin, digoxigenin, or fluorescently labeled nucleotides).\u003cbr\u003e- Compatible with a variety of buffer systems, including buffers for restriction enzymes, PCR, and RT reactions.\u003c\/p\u003e\n\u003cp\u003eScope of Application \u003cbr\u003e- Used in conjunction with DNase, for DNA labeling through nick translation.\u003cbr\u003e- Used in conjunction with RNase H, for the synthesis of the second strand of cDNA.\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"500 U","offer_id":41511820492934,"sku":"E1021-A","price":75.0,"currency_code":"USD","in_stock":true},{"title":"2,500 U","offer_id":41511820525702,"sku":"E1021-B","price":294.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_12982db7-e0b6-412f-897b-2890a6d9d763.jpg?v=1736489018"},{"product_id":"t7-rna-polymerase","title":"T7 RNA Polymerase","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConcentration: 20 U\/\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eProduct Description \u003cbr\u003e\u003cspan\u003ePhage T7 RNA polymerase is a DNA-dependent RNA polymerase that has strict specificity for its corresponding double-stranded promoter. It catalyzes the synthesis of RNA in the 5'\u003c\/span\u003e\u003cspan\u003e→\u003c\/span\u003e\u003cspan\u003e3' direction downstream of the promoter, starting from the promoter on single-stranded DNA or double-stranded DNA.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eSource\u003cbr\u003eRecombinant E. coli strain containing the cloned gene encoding this enzyme.\u003c\/p\u003e\n\u003cp\u003eUnit Definition\u003cbr\u003e\u003cspan\u003eA unit is defined as the amount of enzyme required to incorporate 1 nmol of AMP into a polynucleotide substrate within 60 minutes at 37\u003c\/span\u003e\u003cspan\u003e°\u003c\/span\u003e\u003cspan\u003eC.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003e- Capable of incorporating modified nucleotides (such as aminoallyl, biotin, fluorescein, digoxigenin-labeled nucleotides).\u003c\/p\u003e\n\u003cp\u003eScope of Application \u003cbr\u003eThe synthesis of unlabeled and labeled RNA can be used for:\u003cbr\u003eHybridization, in vitro RNA translation\u003cbr\u003eAs a substrate in aRNA, siRNA, RNase protection assays, and as a template for genomic DNA sequencing\u003cbr\u003eRNA splicing in studies of RNA secondary structure and RNA-protein interactions.\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"5,000 U","offer_id":41511820558470,"sku":"E1022-A","price":155.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_0cee4ff4-2cb3-4e5a-8ef1-415f8ea05e42.jpg?v=1736489021"},{"product_id":"multi-dna-seamless-cloning-mix","title":"Multi-DNA SeamLess Cloning Mix","description":"\u003cp\u003eMulti-DNA Seamless Cloning Mix is an efficient seamless cloning kit designed for the seamless insertion of single or multiple DNA fragments into vectors. The product uses advanced seamless cloning technology, enabling the rapid and precise assembly of DNA fragments. It is the ideal choice for high-efficiency cloning, multi-fragment DNA assembly, and directed mutagenesis. Unlike traditional cloning methods, which require multiple steps (such as restriction enzyme digestion and ligation), this kit simplifies the entire process into a single reaction, ensuring faster and more reliable cloning results. It contains high-fidelity Taq DNA Ligase, enabling single-fragment recombination in as little as 5 minutes with a positive rate of ≥95%.\u003c\/p\u003e\n\u003cp\u003eApplications\u003cbr\u003eMulti-fragment DNA assembly; Rapid cloning; Directed mutagenesis construction.\u003c\/p\u003e\n\u003cp\u003eProduct Features\u003cbr\u003e1. Efficient Multi-fragment Cloning: Supports the assembly of up to 5 DNA fragments in a single reaction, suitable for multi-fragment DNA assembly.\u003cbr\u003e2. Fast and Simple: Simplifies the cloning process, reduces background noise, and enables single-fragment cloning in as little as 5 minutes.\u003cbr\u003e3. High Fidelity: Utilizes HiFi Taq DNA Ligase, improving cloning accuracy and success rates, even for large or complex fragments.\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"CL1031\/50 rxns","offer_id":42151157596294,"sku":"CL1031","price":165.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_0cee4ff4-2cb3-4e5a-8ef1-415f8ea05e42.jpg?v=1736489021"}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/collections\/R4001-2.jpg?v=1730086997","url":"https:\/\/www.gdsbio.com\/collections\/tool-enzyme.oembed","provider":"GDSBio","version":"1.0","type":"link"}