 |
Molecular Biology Grade S1 Nuclease
Product Details:
| Place of Origin: | China |
| Brand Name: | GDSBio |
| Certification: | / |
| Model Number: | E1017 |
Payment & Shipping Terms:
| Minimum Order Quantity: | 10000 U |
|---|---|
| Price: | USD 58-64.5/10000 U |
| Packaging Details: | small package or bulk distribute or OEM |
| Delivery Time: | 8 work days |
| Payment Terms: | L/C, D/A, D/P, T/T, Western Union, MoneyGram |
| Supply Ability: | 100 Bag/Bags Per Day |
|
Detail Information |
|||
| Product Name: | S1 Nuclease | Cat. No./Spec.: | E1017-A/10,000 U |
|---|---|---|---|
| Appearance: | Colourless | Application: | Degrade Single-stranded Nucleic Acids |
| Classification: | General Reagents | Grade: | Molecular Biology |
| Logo Printing: | With Logo Printing | Transport Package: | Packing |
| Production Capacity: | 100 Bag/Bags Per Day | Storage Conditions: | Store At -20°C |
| High Light: | S1 Nuclease for General Reagents,Molecular Biology Grade S1 Nuclease |
||
Product Description
S1 Nuclease
Cat. No./Spec.: E1017-A/10,000 U
Concentration: 100 U/µL
Product Description
S1 nuclease can degrade single-stranded nucleic acids, releasing 5'-phosphorylated mononucleotides or oligonucleotides. Its activity on DNA is five times that on RNA. S1 nuclease can also cleave double-stranded DNA (dsDNA) at single-stranded regions caused by nicks, gaps, mismatches, or loops. S1 nuclease exhibits 3'-phosphomonoesterase activity.
This enzyme is a glycoprotein with a carbohydrate content of 18%.
Cat. No./Spec.: E1017-A/10,000 U
Concentration: 100 U/µL
Product Description
S1 nuclease can degrade single-stranded nucleic acids, releasing 5'-phosphorylated mononucleotides or oligonucleotides. Its activity on DNA is five times that on RNA. S1 nuclease can also cleave double-stranded DNA (dsDNA) at single-stranded regions caused by nicks, gaps, mismatches, or loops. S1 nuclease exhibits 3'-phosphomonoesterase activity.
This enzyme is a glycoprotein with a carbohydrate content of 18%.
Storage Condition & Shelf Life
Store at -20°C.
Source
Aspergillus oryzae strain.
Unit Definition
A unit is defined as the amount of enzyme required to produce 1 µg of acid-soluble deoxyribonucleotides in 1 minute at 37°C. Enzyme activity is determined in the following mixture: 30 mM sodium acetate (pH 4.5), 50 mM NaCl, 0.1 mM ZnCl2, 5% (v/v) glycerol, and 800 µg/mL heat-denatured calf thymus DNA.
Store at -20°C.
Source
Aspergillus oryzae strain.
Unit Definition
A unit is defined as the amount of enzyme required to produce 1 µg of acid-soluble deoxyribonucleotides in 1 minute at 37°C. Enzyme activity is determined in the following mixture: 30 mM sodium acetate (pH 4.5), 50 mM NaCl, 0.1 mM ZnCl2, 5% (v/v) glycerol, and 800 µg/mL heat-denatured calf thymus DNA.
Scope of Application
- Removal of single-stranded overhangs from DNA fragments
- Localization of S1 transcripts
- Cleavage of hairpin loops
- In conjunction with Exonuclease III, creating unidirectional deletions within DNA fragments
Inhibition and Inactivation
- Inhibitors: metal chelating agents, PPi (inorganic pyrophosphate), Pi (inorganic phosphate), 5'-ribonucleotides, and
deoxyribonucleotides.
- Inactivate by heating at 70°C for 10 minutes in the presence of EDTA.
Want to Know more details about this product
