{"product_id":"direct-one-step-probe-rt-qpcr-kit","title":"Direct One-step Probe RT-qPCR Kit","description":"\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eDirect One-step Probe RT-qPCR Kit is a nucleic acid extraction-free, one-step probe-based RT-qPCR master mix. The Direct One-step Enzyme Mix contains optimized reverse transcriptase, antibody-modified hot-start Taq, and temperature-sensitive UDG enzyme, which eliminates DNA contamination at room temperature and is inactivated during the reverse transcription step. This product exhibits extremely high tolerance to inhibitors and can be used directly for target gene detection in various blood samples, swabs, and saliva. It is compatible with multiplex fluorescent PCR systems, supports fast protocols, and is suitable for pathogen detection, gene expression analysis, and other research and IVD development applications.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003cbr\u003eIVD product development for direct amplification of RNA virus samples and tumor samples.\u003cbr\u003eOne-step RT-qPCR experiments for multiple RNA targets.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eNo nucleic acid extraction required\u003c\/strong\u003e: Directly compatible with crude samples (e.g., whole blood, serum, saliva).\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eHigh inhibitor tolerance\u003c\/strong\u003e: Robust performance in complex sample matrices.\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eSupports fast cycling protocols\u003c\/strong\u003e: Ideal for rapid pathogen detection.\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eRoom-temperature UDG treatment\u003c\/strong\u003e: Prevents DNA contamination effectively.\u003c\/p\u003e\n\u003cp class=\"ds-markdown-paragraph\"\u003e\u003cstrong\u003eSuitable for IVD development and research\u003c\/strong\u003e: Especially for RNA virus detection and direct amplification from tumor samples.\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e \u003c\/p\u003e","brand":"GDSBio","offers":[{"title":"V5014-A\/1000rxns (50-μl rxn)","offer_id":42412453200006,"sku":"V5014-A","price":3970.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/1-step3en.jpg?v=1756779152","url":"https:\/\/www.gdsbio.com\/products\/direct-one-step-probe-rt-qpcr-kit","provider":"GDSBio","version":"1.0","type":"link"}