{"product_id":"dna-polymerase-i","title":"DNA Polymerase I","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConcentration: 10 U\/\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eProduct Description \u003cbr\u003e\u003cspan\u003eThis product is a template-dependent DNA polymerase capable of accelerating the synthesis of DNA in the 5'\u003c\/span\u003e\u003cspan\u003e→\u003c\/span\u003e\u003cspan\u003e3' direction. It also possesses 3'\u003c\/span\u003e\u003cspan\u003e→\u003c\/span\u003e\u003cspan\u003e5' exonuclease (proofreading) activity, 5'\u003c\/span\u003e\u003cspan\u003e→\u003c\/span\u003e\u003cspan\u003e3' exonuclease activity, and RNase H activity.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eSource\u003cbr\u003eRecombinant E. coli strain containing the cloned gene polA from E. coli.\u003c\/p\u003e\n\u003cp\u003eUnit Definition\u003cbr\u003e\u003cspan\u003eA unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of deoxynucleotides into a polynucleotide substrate within 30 minutes at 37\u003c\/span\u003e\u003cspan\u003e°\u003c\/span\u003e\u003cspan\u003eC.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003e- Incorporation of modified nucleotides (such as biotin, digoxigenin, or fluorescently labeled nucleotides).\u003cbr\u003e- Compatible with a variety of buffer systems, including buffers for restriction enzymes, PCR, and RT reactions.\u003c\/p\u003e\n\u003cp\u003eScope of Application \u003cbr\u003e- Used in conjunction with DNase, for DNA labeling through nick translation.\u003cbr\u003e- Used in conjunction with RNase H, for the synthesis of the second strand of cDNA.\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"500 U","offer_id":41511820492934,"sku":"E1021-A","price":75.0,"currency_code":"USD","in_stock":true},{"title":"2,500 U","offer_id":41511820525702,"sku":"E1021-B","price":294.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_12982db7-e0b6-412f-897b-2890a6d9d763.jpg?v=1736489018","url":"https:\/\/www.gdsbio.com\/products\/dna-polymerase-i","provider":"GDSBio","version":"1.0","type":"link"}