{"product_id":"dnase-i-rnase-free-hc","title":"DNase I, RNase-Free, HC","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConcentration: 50 U\/\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eProduct Description \u003cbr\u003eDNase I (without RNase) is an endonuclease that can cleave both single-stranded and double-stranded DNA. It hydrolyzes phosphodiester bonds, producing single deoxyribonucleotides and oligodeoxyribonucleotides with 5'-phosphate groups and 3'-OH groups.\u003cbr\u003eThe activity of this enzyme is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions. In the presence of Mg2+, DNase I cuts each strand of dsDNA in a statistically random and independent manner. When Mn2+ is present, the enzyme almost cuts both DNA strands at the same site, resulting in DNA fragments with blunt ends or overhangs of one or a few nucleotides.\u003c\/p\u003e\n\u003cp\u003eSource\u003cbr\u003eRecombinant E. coli strain containing the cloned gene encoding bovine DNase I.\u003c\/p\u003e\n\u003cp\u003eUnit Definition\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA unit is defined as the amount of enzyme required to completely degrade 1 µg of plasmid DNA within 1 minute at 37°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEnzyme activity is determined in the following mixture: 10 mM Tris-HCl (pH 7.5 at 25°C), 2.5 mM MgCl\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e, 0.1 mM CaCl\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e, and 1 µg of pUC19 DNA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eOne unit of DNase I is equivalent to 0.3 Kunitz units.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003e- Recombinant enzyme\u003cbr\u003e- Purified from a non-animal host, with low levels of endogenous RNase\u003c\/p\u003e\n\u003cp\u003eScope of Application \u003cbr\u003e- For the preparation of DNA-free RNA.\u003cbr\u003e- To remove template DNA after in vitro transcription.\u003cbr\u003e- To prepare DNA-free RNA before RT-PCR and RT-qPCR.\u003cbr\u003e- In conjunction with DNA Polymerase I for DNA labeling through nick translation.\u003cbr\u003e- For the study of DNA-protein interactions using DNase I (RNase-free) footprinting.\u003cbr\u003e- To generate a library of randomly sheared DNA insert fragments. Reaction buffer containing Mn2+ is used.\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"1,000 U","offer_id":41511820263558,"sku":"E1018-A","price":98.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_55d87818-944f-4e6b-b991-27c5cdc62a64.jpg?v=1736489008","url":"https:\/\/www.gdsbio.com\/products\/dnase-i-rnase-free-hc","provider":"GDSBio","version":"1.0","type":"link"}