{"product_id":"mirna-1st-strand-cdna-synthesis-kit-by-tailing-a","title":"miRNA 1st Strand cDNA Synthesis Kit by tailing A","description":"\u003cp class=\"MsoNormal\"\u003emiRNA 1st Strand cDNA Synthesis Kit (by tailing A) is a polyadenylation-based reverse transcription kit for miRNA, also known as a first-strand cDNA synthesis kit. This kit uses miRNA or total RNA as a template. First, under the action of Poly A polymerase (PAP), poly (A) tails are added to the 3' end of miRNA to complete the polyadenylation reaction. Subsequently, a reverse transcriptase specifically engineered for miRNA reverse transcription and a universal reverse transcription primer are used, along with an optimized buffer system, to perform a highly sensitive reverse transcription reaction. Both the polyadenylation and reverse transcription reactions are carried out in the same tube, making the process convenient, time-saving, and reducing the risk of contamination.\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003ePolyadenylation-based first-strand cDNA synthesis from total RNA or miRNA.\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"R1101\/50 rxns","offer_id":41707580031110,"sku":"R1101","price":275.0,"currency_code":"USD","in_stock":true},{"title":"R1102\/100 rxns","offer_id":41707580063878,"sku":"R1102","price":575.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/R1031-1_77290c6f-603a-4338-b223-2e5db91abf3e.jpg?v=1729503202","url":"https:\/\/www.gdsbio.com\/products\/mirna-1st-strand-cdna-synthesis-kit-by-tailing-a","provider":"GDSBio","version":"1.0","type":"link"}