{"product_id":"one-step-probe-rt-qpcr-kit-v3","title":"One-step Probe RT-qPCR Kit V3","description":"\u003cp\u003eOne-step Probe RT-qPCR Kit V3 is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening\/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP\/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55\u003cspan\u003e℃\u003c\/span\u003e, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Integrating the superior performance of Reverse Transcriptase and hot-start Taq DNA Polymerase, with the optimized buffer system, the detection sensitivity of One-step Probe RT-qPCR Kit can reach 0.1 pg total RNA or \u0026lt;10 copies of RNA template. The kit is available as a convenient Master Mix. 5X One-step U+ Mix contains an optimized buffer system and dNTP\/dUTP Mix, which is suitable for high-specificity, low template concentration and multiple detection systems with fluorescent labeled probes such as TaqMan. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003eRNA virus \u0026amp; pathogen detection – e.g., SARS-CoV-2, HIV, influenza, dengue, HCV\u003cbr\u003eGene expression quantification – from low-abundance or precious RNA samples\u003cbr\u003eMultiplex target detection – simultaneous detection of 4–6 targets in one tube\u003cbr\u003eDirect amplification (no RNA extraction) – using crude lysates from swabs, blood, tissues\u003cbr\u003eLow-abundance \u0026amp; high-GC content RNA templates – high sensitivity down to a few copies or 0.1 pg total RNA\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003eFast start-up, 30s hot start\u003cbr\u003eReverse transcription at 55\u003cspan\u003e℃\u003c\/span\u003e to accommodate complex RNA templates\u003cbr\u003eContains heat-labile UDG to reduce false-positive contamination\u003cbr\u003eHigh sensitivity for detection of low-abundance genes\u003cbr\u003eHigh stability, unchanged performance at 4\u003cspan\u003e℃\u003c\/span\u003e and 37\u003cspan\u003e℃\u003c\/span\u003e for one week\u003cbr\u003eThe reaction system can be formulated up to 30 minutes in advance without changing performance\u003cbr\u003eSaves time by supporting fast program\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/20260127172412.png?v=1769678791\" alt=\"\"\u003e\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"V5011\/200 rxns","offer_id":41401654149254,"sku":"V5011","price":351.0,"currency_code":"USD","in_stock":true},{"title":"V5011-2\/1000 rxns","offer_id":41401654182022,"sku":"V5011-2","price":1228.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/V5011-1_50b8c22b-dbc9-4f8c-85a8-5948a5e0f1d4.jpg?v=1729499775","url":"https:\/\/www.gdsbio.com\/products\/one-step-probe-rt-qpcr-kit-v3","provider":"GDSBio","version":"1.0","type":"link"}