{"product_id":"one-step-probe-rt-qpcr-kit","title":"One-step Probe RT-qPCR Kit","description":"\u003cp\u003eOne-step Probe RT-qPCR Kit is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening\/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP\/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55℃, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Integrating the superior performance of Reverse Transcriptase and hot-start Taq DNA Polymerase, with the optimized buffer system, the detection sensitivity of One-step Probe RT-qPCR Kit can reach 0.1 pg total RNA or \u0026lt;10 copies of RNA template. The kit is available as a convenient Master Mix. 2X One-step U+ Mix contains an optimized buffer system and dNTP\/dUTP Mix, which is suitable for high-specificity detection systems with fluorescent labeled probes such as TaqMan?. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003eRNA virus \u0026amp; pathogen detection – e.g., SARS-CoV-2, HIV, influenza, dengue, HCV\u003cbr\u003eGene expression quantification – from low-abundance or precious RNA samples\u003cbr\u003eMultiplex target detection – simultaneous detection of 4–6 targets in one tube\u003cbr\u003eDirect amplification (no RNA extraction) – using crude lysates from swabs, blood, tissues\u003cbr\u003eLow-abundance \u0026amp; high-GC content RNA templates – high sensitivity down to a few copies or 0.1 pg total RNA\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003eOne-step RT-qPCR\u003cbr\u003eThis kit is suitable for fluorescence quantification by probe method\u003cbr\u003eThis kit is compatible with many real-time systems\u003cbr\u003eHot-start technology brings high specificity and reproducible amplification\u003cbr\u003edUTP\/UDG system, effectively prevent PCR product contamination\u003c\/p\u003e\n\u003cp\u003e\u003cimg alt=\"\" src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/20260127172412.png?v=1769678791\"\u003e\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"V5001\/200 rxns","offer_id":41401653657734,"sku":"V5001","price":200.0,"currency_code":"USD","in_stock":true},{"title":"V5002\/5000 rxns","offer_id":41401653690502,"sku":"V5002","price":4000.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/V5001-1_07c138f6-378c-4036-beba-be9cf8c4c4ca.jpg?v=1729499757","url":"https:\/\/www.gdsbio.com\/products\/one-step-probe-rt-qpcr-kit","provider":"GDSBio","version":"1.0","type":"link"}