{"product_id":"proteinase-k-pcr-grade","title":"Proteinase K (recombinant), PCR grade","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConcentration: \u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e≥\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003e 600 U\/m\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eL (~20 mg\/mL)\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eProduct Description \u003cbr\u003eProteinase K is an endopeptidase capable of hydrolyzing peptide bonds at the carboxyl terminus of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K belongs to the family of serine proteases. This enzyme can minimally hydrolyze tetra-peptides.\u003c\/p\u003e\n\u003cp\u003eSource\u003cbr\u003eRecombinant Pichia pastoris strain containing a cloned gene from Tritirachium album.\u003c\/p\u003e\n\u003cp\u003eUnit Definition\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA unit is defined as the amount of enzyme required to release an amount of Folin-positive amino acids and peptides equivalent to 1 \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003emol of tyrosine from denatured hemoglobin in 1 minute at 37°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEnzyme activity is determined in the following mixture: 0.08M potassium phosphate (pH 7.5), 5M urea, 4mM NaCl, 3mM CaCl\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e, and 16.7 mg\/mL hemoglobin.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003e- Ready-to-use solution\u003cbr\u003e- Compatible with a wide range of reaction conditions\u003c\/p\u003e\n\u003cp\u003eScope of Application \u003cbr\u003e- Isolate genomic DNA from mouse tails.\u003cbr\u003e- Isolate genomic DNA from cultured cells.\u003cbr\u003e- Eliminate DNase and RNase during the isolation of DNA and RNA from tissues or cell lines.\u003cbr\u003e- Determine the location of enzymes.\u003cbr\u003e- Improve the cloning efficiency of PCR products.\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"E1019-A\/1 mL","offer_id":41511820361862,"sku":"E1019-A","price":55.0,"currency_code":"USD","in_stock":true},{"title":"E1019-B\/1 mL*5","offer_id":41511820394630,"sku":"E1019-B","price":250.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_725f78e5-1ff3-45e0-bff2-0489a6d417ce.jpg?v=1736489012","url":"https:\/\/www.gdsbio.com\/products\/proteinase-k-pcr-grade","provider":"GDSBio","version":"1.0","type":"link"}