{"product_id":"rnase-h","title":"RNase H","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConcentration: 5 U\/\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eProduct Description \u003cbr\u003eRibonuclease H (RNase H) can specifically degrade the RNA strand within RNA-DNA hybrids. This enzyme does not hydrolyze the phosphodiester bonds in single-stranded or double-stranded DNA and RNA.\u003c\/p\u003e\n\u003cp\u003eSource\u003cbr\u003eE. coli strain MRE-600.\u003c\/p\u003e\n\u003cp\u003eUnit Definition\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA unit is defined as the amount of enzyme required to catalyze the formation of 1 nmol of acid-soluble products within 20 minutes at 37°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEnzyme activity is determined in the following mixture: 20 mM Tris-HCl (pH 7.8), 40 mM KCl, 8 mM MgCl\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e,1 mM DTT, 24 µM [\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e3\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eH]-poly(A)·poly(dT), 0.03 mg\/mL BSA, and 4% (v\/v) glycerol.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eScope of Application \u003cbr\u003e- Removal of mRNA before the synthesis of the second-strand cDNA\u003cbr\u003e- RT-PCR and qRT-PCR: Removal of RNA after the first-strand cDNA synthesis\u003cbr\u003e- Removal of poly(A) sequence from mRNA after hybridization with Oligo(dT)\u003cbr\u003e- Site-specific cleavage of RNA\u003cbr\u003e- Study of products from in vitro polyadenylation reactions\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"E1016-A\/100 U","offer_id":41511820165254,"sku":"E1016-A","price":34.0,"currency_code":"USD","in_stock":true},{"title":"E1016-B\/500 U","offer_id":41511820198022,"sku":"E1016-B","price":128.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_a798d2db-fb30-41ee-8438-2b25c10c5296.jpg?v=1736489001","url":"https:\/\/www.gdsbio.com\/products\/rnase-h","provider":"GDSBio","version":"1.0","type":"link"}