{"product_id":"s1-nuclease","title":"S1 Nuclease","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConcentration: 100 U\/\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eProduct Description \u003cbr\u003eS1 nuclease can degrade single-stranded nucleic acids, releasing 5'-phosphorylated mononucleotides or oligonucleotides. Its activity on DNA is five times that on RNA. S1 nuclease can also cleave double-stranded DNA (dsDNA) at single-stranded regions caused by nicks, gaps, mismatches, or loops. S1 nuclease exhibits 3'-phosphomonoesterase activity.\u003cbr\u003eThis enzyme is a glycoprotein with a carbohydrate content of 18%.\u003c\/p\u003e\n\u003cp\u003eSource\u003cbr\u003eAspergillus oryzae strain.\u003c\/p\u003e\n\u003cp\u003eUnit Definition\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA unit is defined as the amount of enzyme required to produce 1 \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003eg of acid-soluble deoxyribonucleotides in 1 minute at 37°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEnzyme activity is determined in the following mixture: 30 mM sodium acetate (pH 4.5), 50 mM NaCl, 0.1 mM ZnCl\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e, 5% (v\/v) glycerol, and 800 \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003eg\/mL heat-denatured calf thymus DNA.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eScope of Application \u003cbr\u003e- Removal of single-stranded overhangs from DNA fragments\u003cbr\u003e- Localization of S1 transcripts\u003cbr\u003e- Cleavage of hairpin loops\u003cbr\u003e- In conjunction with Exonuclease III, creating unidirectional deletions within DNA fragments\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"E1017-A\/10,000 U","offer_id":41511820230790,"sku":"E1017-A","price":73.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_0eb42cba-2125-4e4c-a00f-d1dc411c16ac.jpg?v=1736489005","url":"https:\/\/www.gdsbio.com\/products\/s1-nuclease","provider":"GDSBio","version":"1.0","type":"link"}