{"product_id":"super-4x-one-step-multiplex-master-mix","title":"Super 4X One-Step Multiplex Master Mix","description":"\u003cp\u003eSuper 4X One-Step Multiplex Master Mix is used to perform one-step multiplex real-time PCR applications with any gene-specific primer and probe sets, and is suitable for both RNA and DNA targets. Dual hot-start reverse transcriptase and DNA polymerase can complete more than 10-plex highly sensitive and specific quantitative PCR in a single system. The master mix is supplied at a 4X concentration that allows to input more sample into each reaction, increasing sensitivity even in low-volume reactions.\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003eUltra‑multiplex pathogen detection – Detects \u0026gt;10 RNA\/DNA targets in one tube (e.g., respiratory viruses, bloodborne pathogens).\u003cbr\u003eHigh sensitivity with low‑abundance targets – 4X concentration allows more sample input, ideal for liquid biopsy, low‑copy viruses, or precious specimens.\u003cbr\u003eGene expression \u0026amp; biomarker panels – Simultaneously quantifies multiple mRNAs (e.g., cytokines, oncogenes) from limited RNA samples.\u003cbr\u003eSmall‑volume \u0026amp; high‑throughput qPCR – Works in 5‑10 µL reactions, suitable for 384‑well plates, microfluidics, or portable qPCR devices.\u003cbr\u003eDirect amplification from crude samples – Tolerates inhibitors, enabling one‑step testing from swabs, blood lysates, or environmental samples without RNA extraction.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003esupport 10-plex amplification with high specificity\u003cbr\u003eReverse transcription at 55 ¡ãC to accommodate complex RNA templates\u003cbr\u003eContains heat-labile UDG to reduce false-positive contamination\u003cbr\u003eHigh sensitivity for detection of low-abundance genes\u003cbr\u003eHigh stability\u003cbr\u003e4X formation, high concentration\u003cbr\u003eSaves time by supporting fast program\u003c\/p\u003e\n\u003cp\u003e\u003cimg src=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/20260127172412.png?v=1769678791\" alt=\"\"\u003e\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"V5012-A\/200 rxns","offer_id":41401654214790,"sku":"V5012-A","price":475.0,"currency_code":"USD","in_stock":true},{"title":"V5012-B\/5000 rxns","offer_id":41401654247558,"sku":"V5012-B","price":9350.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/V5012-1_3c0dab66-71f2-4317-9b40-a427677ec6bd.jpg?v=1729499778","url":"https:\/\/www.gdsbio.com\/products\/super-4x-one-step-multiplex-master-mix","provider":"GDSBio","version":"1.0","type":"link"}