{"product_id":"t4-b-glucosyltransferase","title":"T4 β-Glucosyltransferase","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConcentration: 5 U\/\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eProduct Description \u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eT4 β-glucosyltransferase (T4 BGT) is capable of transferring the glucosyl group from uridine diphosphate glucose (UDP-glucose) to the 5-hydroxymethylcytosine (5-hmC) residues within double-stranded DNA, resulting in the formation of β-glucosyl-5-hydroxymethylcytosine. This enzyme is formulated for rapid reaction times without compromising the efficiency of the reaction. It can glucosylate 5-hmC on 1 \u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eg of DNA in 15 minutes at 37°C.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eUnit Definition\u003cbr\u003e\u003cspan\u003eA unit is defined as the amount of enzyme required to protect 0.5 \u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eg of fully 5-hydroxymethylated 1095bp PCR fragment from digestion by MunI within 1 hour at 37°C in a 50 \u003c\/span\u003e\u003cspan\u003eμ\u003c\/span\u003e\u003cspan\u003eL volume of the recommended reaction buffer.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003e- Specificity - Selectively transfers glucose to the hydroxymethyl group of 5-hmC.\u003cbr\u003e- Speed - Completes the glucosylation of 1 \u003cspan\u003eμ\u003c\/span\u003eg DNA in 15 minutes.\u003cbr\u003e- Convenience - Comes with optimized buffer and UDP-glucose included.\u003c\/p\u003e\n\u003cp\u003eScope of Application \u003cbr\u003e- Site-specific detection of 5-hmC.\u003cbr\u003e- Enrichment of DNA containing 5-hmC.\u003cbr\u003e- Labeling of 5-hmC residues using radioactive UDP-glucose donor.\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"E1020-A\/500 U","offer_id":41511820460166,"sku":"E1020-A","price":272.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_3115bf98-382f-47df-b0f6-ab880cc18ba6.jpg?v=1736489015","url":"https:\/\/www.gdsbio.com\/products\/t4-b-glucosyltransferase","provider":"GDSBio","version":"1.0","type":"link"}