{"product_id":"t4-dna-ligase-fast","title":"T4 DNA Ligase (Fast)","description":"\u003cp\u003eT4 DNA Ligase (Fast) is produced by E. coli carrying T4 bacteriophage gene 30. This enzyme catalyzes the formation of phosphodiester bonds between the 5'-phosphate group and the 3'-hydroxyl group of double-helix DNA or RNA. This enzyme can repair single-strand breaks in double-stranded DNA, RNA, or DNA\/RNA complexes, and can connect DNA fragments with sticky or blunt ends, but it is inactive for single-stranded nucleic acids. It is mainly used for cloning of restriction enzyme digestion products, gene site-directed mutagenesis, PCR product cloning, linear DNA self-looping, and repair of double-stranded DNA breaks. T4 DNA Ligase (Fast) requires ATP as a cofactor and completes the sticky end ligation reaction at room temperature in only 10 minutes.\u003c\/p\u003e\n\u003cp\u003econcentration: 5U\/µL\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003e1.TA clone\u003cbr\u003e2.NGS adapter ligation\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003eHigh ligation activity\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"1,000 U","offer_id":41420075761798,"sku":"K012","price":105.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_bb38757d-7050-44e4-83fe-b12b02547583.jpg?v=1730706660","url":"https:\/\/www.gdsbio.com\/products\/t4-dna-ligase-fast","provider":"GDSBio","version":"1.0","type":"link"}