{"product_id":"t4-dna-ligase-lc","title":"T4 DNA Ligase, LC","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConcentration: 1 Weiss U\/\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eT4 DNA Ligase catalyzes the formation of phosphodiester bonds between juxtaposed 5'-phosphate and 3'-hydroxyl termini in double-stranded DNA or RNA. This enzyme is capable of repairing single-strand nicks in double-stranded DNA, RNA, or DNA\/RNA hybrids, and can join DNA fragments with sticky or blunt ends, but it is inactive on single-stranded nucleic acids.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eT4 DNA Ligase requires ATP as a cofactor.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eSource\u003cbr\u003eRecombinant E. coli strain containing the gene 30 cloned from bacteriophage T4.\u003cbr\u003e\u003cbr\u003eUnit Definition\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eAt 37\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e, 1 Weiss unit of enzyme catalyzes the conversion of 1 nmol of [\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e32\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003ePPi] into an activated carbon adsorption state within 20 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEnzyme activity is determined in the following mixture: 66 mM Tris-HCl (pH 7.6), 6.6 mM MgCl\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e, 0.066 mM ATP, 10 mM DTT, 3.3 μM [\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e32\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eP]orthophosphate (PPi).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eOne Weiss unit is equivalent to approximately 200 cohesive end ligation units (CEU), which is equivalent to the number of HindIII digested DNA fragments that can be ligated within 30 minutes at 16\u003c\/span\u003e\u003cspan\u003e°C\u003c\/span\u003e\u003cspan\u003e. λ DNA fragments.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eScope of Application \u003cbr\u003e- DNA fragments generated by cloning restriction enzymes\u003cbr\u003e- Cloned PCR products\u003cbr\u003e- Ligation of double-stranded oligonucleotides or adapters to DNA\u003cbr\u003e- Site-directed mutagenesis\u003cbr\u003e- Amplified fragment length polymorphism (AFLP)\u003cbr\u003e- Ligase-mediated RNA detection\u003cbr\u003e- Nick repair in double-stranded DNA, RNA, or DNA\/RNA hybrids\u003cbr\u003e- Self-circularization of linear DNA\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"E1014-E\/1,000 U","offer_id":41511818133638,"sku":"E1014-E","price":137.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_7fe6f826-ee9b-4a06-932b-f9b950923ed8.jpg?v=1736488995","url":"https:\/\/www.gdsbio.com\/products\/t4-dna-ligase-lc","provider":"GDSBio","version":"1.0","type":"link"}