{"product_id":"t4-dna-polymerase","title":"T4 DNA Polymerase","description":"\u003cp\u003eIn the presence of templates and primers, T4 DNA Polymerase catalyzes DNA synthesis in the 5→3 direction. This enzyme also has the activity of 3→5 exonuclease which is stronger than E. coli DNA polymerase I. Unlike DNA polymerase I, T4 DNA Polymerase does not have 5→3 exonuclease activity.\u003c\/p\u003e\n\u003cp\u003e5U\/µL\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003e1. Removal of 3'- overhangs to form blunt ends; \u003cbr\u003e2. Fill-in of 5'- overhangs to form blunt ends; \u003cbr\u003e3. Labeling DNA probe synthesis through displacement reaction; \u003cbr\u003e4. Synthesis of the second strand during site-directed mutagenesis; \u003cbr\u003e5. PCR product cloning without relying on ligation reactions.\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003e5' →3' polymerase activity\u003cbr\u003e3' →5' exonuclease activity\u003cbr\u003eNo 5' →3' exonuclease activity\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"K011-A\/100 U","offer_id":41420075630726,"sku":"K011-A","price":49.0,"currency_code":"USD","in_stock":true},{"title":"K011-B\/500 U","offer_id":41420075663494,"sku":"K011-B","price":185.0,"currency_code":"USD","in_stock":true},{"title":"K011-C\/2,000 U","offer_id":41420075696262,"sku":"K011-C","price":523.0,"currency_code":"USD","in_stock":true},{"title":"K011-D\/5,000 U","offer_id":41420075729030,"sku":"K011-D","price":977.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_1ffe31bc-0a83-4b51-8294-0dc2a0701703.jpg?v=1730706657","url":"https:\/\/www.gdsbio.com\/products\/t4-dna-polymerase","provider":"GDSBio","version":"1.0","type":"link"}