{"product_id":"t4-polynucleotide-kinase","title":"T4 Polynucleotide Kinase","description":"\u003cp\u003eT4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer and exchange of Pi from the γ  position of ATP to the 5'-hydroxyl terminus of polynucleotides (double-stranded and single-stranded DNA and RNA) and nucleoside 3'-monophosphate. T4 polynucleotide kinase also catalyzes the removal of 3'-phosphoryl groups from 3'-phosphate-based polynucleotides, deoxynucleoside 3'-monophosphate, and deoxynucleoside 3' -diphosphate. When ADP is present, T4 PNK has 5'-end phosphatase activity, catalyzing the exchange of 5'-P-oligomeric\/polymeric nucleotides and ATP terminal 5'-phosphate groups. It is suitable for constructing DNA libraries and producing probes with terminal labeling.\u003c\/p\u003e\n\u003cp\u003e10U\/µL\u003c\/p\u003e\n\u003cp\u003eApplication\u003cbr\u003e1. End labeling DNA or RNA for probes and DNA sequencing; \u003cbr\u003e2. 5' phosphorylation of DNA\/RNA for subsequent ligation; \u003cbr\u003e3. Phosphorylate the 5' end of the 3' phosphorylated mononucleotide to prepare the pNp substrate for addition to the 3' end of DNA or RNA; \u003cbr\u003e4. Label the 5' end of oligonucleotides with a phosphate group at the 3' end.\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003ePhosphorylation of the 5' end of DNA or RNA for ligation reactions\u003cbr\u003eTerminal labeling of DNA or RNA for use as probes and DNA sequencing\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"50 U","offer_id":41420075794566,"sku":"K013-A","price":49.0,"currency_code":"USD","in_stock":true},{"title":"2500 U","offer_id":41420075827334,"sku":"K013-B","price":132.0,"currency_code":"USD","in_stock":true},{"title":"10,000 U","offer_id":41420075860102,"sku":"K013-C","price":391.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_37df291e-c0c3-4a43-823b-60f90ab1741a.jpg?v=1730706662","url":"https:\/\/www.gdsbio.com\/products\/t4-polynucleotide-kinase","provider":"GDSBio","version":"1.0","type":"link"}