{"product_id":"t4-rna-ligase","title":"T4 RNA Ligase","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConcentration: 10 U\/\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eT4 RNA Ligase catalyzes the ATP-dependent formation of phosphodiester bonds between 5'-phosphate groups and 3'-hydroxyl termini of oligonucleotides, single-stranded RNA, and DNA molecules both intermolecularly and intramolecularly.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eMinimum substrates: a nucleotide 3',5'-phosphate in intermolecular reactions and an 8-nucleotide oligonucleotide in intramolecular reactions.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eSource\u003cbr\u003eRecombinant E. coli strain contains the gene 63 cloned from bacteriophage T4.\u003c\/p\u003e\n\u003cp\u003eUnit Definition\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eA unit is defined as the amount of enzyme required to catalyze the conversion of 1 nmol of 5'-[\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e32\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eP]-(A)\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e12-18\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e to its acid-resistant form at 37°C within 30 minutes.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cspan\u003eEnzyme activity is determined in the following mixture: 50 mM Tris-HCl (pH 7.5), 10 mM MgCl\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e2\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e, 10 mM DTT, 1 mM ATP, and 10 \u003c\/span\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003cspan\u003eM 5'-[\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e32\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eP]-(A)\u003c\/span\u003e\u003csub\u003e\u003cspan\u003e12-18\u003c\/span\u003e\u003c\/sub\u003e\u003cspan\u003e (10 µM at the 5'-terminus).\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eScope of Application \u003cbr\u003e- RNA 3'-terminal labeling (using cytidine \u003cspan\u003e3',5'-di[α-\u003c\/span\u003e\u003csup\u003e\u003cspan\u003e32\u003c\/span\u003e\u003c\/sup\u003e\u003cspan\u003eP]\u003c\/span\u003e phosphate)\u003cbr\u003e- RNA-to-RNA ligation\u003cbr\u003e- Synthesis of oligoribonucleotides and oligodeoxyribonucleotides\u003cbr\u003e- Specific modification of tRNA\u003cbr\u003e- Ligation of oligodeoxynucleotides to single-stranded cDNA for 5' RACE (Rapid Amplification of cDNA Ends)\u003cbr\u003e- Site-specific generation of PCR primer complexes\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"E1015-A\/1,000 U","offer_id":41511818166406,"sku":"E1015-A","price":143.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_bb37d985-398c-4170-b1c9-8741ee6ea60a.jpg?v=1736488998","url":"https:\/\/www.gdsbio.com\/products\/t4-rna-ligase","provider":"GDSBio","version":"1.0","type":"link"}