{"product_id":"t7-rna-polymerase","title":"T7 RNA Polymerase","description":"\u003cp\u003e\u003cstrong\u003e\u003cspan\u003eConcentration: 20 U\/\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eµ\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan\u003eL\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eProduct Description \u003cbr\u003e\u003cspan\u003ePhage T7 RNA polymerase is a DNA-dependent RNA polymerase that has strict specificity for its corresponding double-stranded promoter. It catalyzes the synthesis of RNA in the 5'\u003c\/span\u003e\u003cspan\u003e→\u003c\/span\u003e\u003cspan\u003e3' direction downstream of the promoter, starting from the promoter on single-stranded DNA or double-stranded DNA.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eSource\u003cbr\u003eRecombinant E. coli strain containing the cloned gene encoding this enzyme.\u003c\/p\u003e\n\u003cp\u003eUnit Definition\u003cbr\u003e\u003cspan\u003eA unit is defined as the amount of enzyme required to incorporate 1 nmol of AMP into a polynucleotide substrate within 60 minutes at 37\u003c\/span\u003e\u003cspan\u003e°\u003c\/span\u003e\u003cspan\u003eC.\u003c\/span\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003eFeatures\u003cbr\u003e- Capable of incorporating modified nucleotides (such as aminoallyl, biotin, fluorescein, digoxigenin-labeled nucleotides).\u003c\/p\u003e\n\u003cp\u003eScope of Application \u003cbr\u003eThe synthesis of unlabeled and labeled RNA can be used for:\u003cbr\u003eHybridization, in vitro RNA translation\u003cbr\u003eAs a substrate in aRNA, siRNA, RNase protection assays, and as a template for genomic DNA sequencing\u003cbr\u003eRNA splicing in studies of RNA secondary structure and RNA-protein interactions.\u003c\/p\u003e","brand":"GDSBIO","offers":[{"title":"5,000 U","offer_id":41511820558470,"sku":"E1022-A","price":155.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0586\/5825\/9078\/files\/ngs_enzyme_0cee4ff4-2cb3-4e5a-8ef1-415f8ea05e42.jpg?v=1736489021","url":"https:\/\/www.gdsbio.com\/products\/t7-rna-polymerase","provider":"GDSBio","version":"1.0","type":"link"}