DNA Library Construction Scheme In One Tube Fast DNA Library Prep Kit V2

DNA Library Construction Scheme In One Tube Fast DNA Library Prep Kit V2

Product Details:

Place of Origin: China
Brand Name: GDSBio
Certification: ISO9001, ISO13485
Model Number: K001S-A, K001S-B

Payment & Shipping Terms:

Minimum Order Quantity: 1 box
Packaging Details: small package or bulk distribute or OEM
Delivery Time: 8 work days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 10000 Box/Boxes per Day
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Detail Information

Stock: Yes Cat. No.: K001S-A, K001S-B
Specification: K001S-A/24 Rxns; K001S-B/96 Rxns; Sample Sack/ 6 Rxns Appearance: Colourless
Applications: DNA-Seq Sequencing Platform: Illumina
Support: Technical Support Shelf Life: 12 Months
Storage Conditions: -20°C
High Light:

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Inactivation Type single-use virus sampling tube

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Product Description

Fast DNA Library Prep Kit V2

【Product Name】

Fast DNA Library Prep Kit V2

【Cat. No./Spec.】

K001S-A/24 rxns; K001S-B/96 rxns; sample sack/ 6 rxns

【Product Description】

Aiming at Illumina high-throughput sequencing platform, this kit provides a convenient and universal DNA library construction scheme in one tube. It combines end repair and A-Tailing into one step, greatly shortening the time of library construction and reducing the error caused by tedious steps. The end preparation, adapter ligation, amplification and purification of the fragmented double-stranded DNA can be performed within about 2 hours. Complete library quantification can be performed by dsDNA fluorescent dye method (e.g., Thermo Qubit Flex Fluorometer) or absolute quantification PCR after diluting the library to an appropriate concentration.

【Sample Type】

Application Sample Type Recommended Amount
Whole genome sequencing High quality complex genomes 50ng-1μg
Target capture sequencing of whole exome High quality complex genomes 10ng-1μg
Target capture sequencing of whole genome FFPE DNA ≥50ng
Target capture sequencing of whole genome cfDNA/ctDNA ≥100pg
Whole genome sequencing Microbial genome 1ng-1μg
Whole genome sequencing(PCR-free) High quality DNA

≥50ng (no size selection)

≥200ng (size selection)

ChIP-Seq ChIP DNA ≥100pg
Targeted sequencing Amplicon ≥100pg

【Storage Condition & Shelf Life】

All reagents should be stored at -20°C. Ligation Buffer is normal for crystals to precipitate at low temperatures, it should be balanced to room temperature before use. The product is valid for 12 months.

【Components】

Component 24 rxns 96 rxns
End Repair & A-Tailing Enzyme Mix 120 μl 2×240 μl
End Repair & A-Tailing Buffer 240 μl 2×480 μl
Fast DNA Ligase 120 μl 2×240 μl
Fast Ligation Buffer 600 μl 4×600 μl
2× HIFI Library PCR Master Mix 600 μl 4×600 μl
Primer Mix* 120 μl 480 μl

* If there are more than one sample, #K002 and #K003 adapter primer mix is recommended. This kit provides a set of primers with index.

Note:recommended selection beads: #NC1011 GDSPure DNA Selection Magbeads or AMPure XP beads.

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Guangzhou Dongsheng Biotechnology Co., Ltd. was founded in 2005, located in Science City, Huangpu District, Guangzhou. It is a high-tech enterprise focusing on the research and development, production and sales of high-quality life science products. The company has a complete product line, with PCR technology as the core, focusing on ordinary PCR, fluorescence quantitative PCR, NGS storage, nucleic acid electrophoresis and other molecular biology technologies, and has developed molecular scientific research reagents, molecular in vitro diagnostic raw materials, nucleic acid extraction and detection reagents and other products. Complete the whole industrial chain layout from basic molecular biology reagents, industrial production raw materials, in vitro diagnostic reagents to professional services.

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