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50 Preps 250 Preps PCR Sample Preparation Solution P9051 P9052

Basic Information
Place of Origin: China
Brand Name: GDSBio
Certification: /
Model Number: P9051/P9052
Minimum Order Quantity: 1Bag
Packaging Details: small package or bulk distribute or OEM
Delivery Time: 8 work days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 100 Bag/Bags Per Day
Detail Information
Cat. No.: P9051/P9052 Specification: 50preps/250preps
Appearance: Colourless Application: Molecular Biology
Classification: General Reagents Grade: Molecular Biology
Logo Printing: With Logo Printing Transport Package: Packing
Production Capacity: 100 Bag/Bags Per Day Storage Conditions: Store At RT
High Light:

250 preps pcr sample preparation

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pcr sample preparation Solution

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50 Preps pcr sample prep


Product Description

PCR Sample Preparation Solution

PCR Sample Preparation Solution P9051 P9052

#P9051, 50 preps

#P9052, 250 preps

 

Components

Content P9051 P9052
Extracion Solution 10 ml 40 ml
Neutralization Solution 1 ml 5 ml

 

Store at RT

For research use only.

 

Quality Control
The absence of endodeoxyribonucleases, exodeoxyribonucleases and ribonucleases is confirmed by appropriate quality tests.

 

Protocol

1. Sample Preparation
a. For tissue
Add 3-5mg of sample tissue to a 1.5ml microcentrifuge tube.
b. For hair
Take take two pieces of hair with follicle. Cut the hair root with follicle into a 1.5ml microcentrifuge tube.

c. For buccal swab

Ensure that person providing the sample has not consumed any food or drink in the 30 minutes prior to the sample collection.

Wipe the buccal inner wall 10 times with aseptic cotton swab. Cut off swab into a 1.5ml microcentrifuge tube.

Add 800 μl TE and mix thoroughly by vortexing. Transfer 500 μl of the swab solution to a new microcentrifuge tube.

Centrifuge for 3 min at 5,000 rpm. Discard the flow-through.

d. For culture cells

For resuspend cells, collect 1 ×103-104 cells to a 50 μl PCR system.

For adherent cells, Add 3-5mg cells to a 1.5ml microcentrifuge tube.

e. For serum, DNA virus

2. Add 180 μl Extracion Solution(90 μl for buccal swab). Mix thoroughly by vortexing. Incubate for 10 minutes at 95 ℃.

3. Add 20 μl Neutralization Solution(10 μl for buccal swab).Mix thoroughly by vortexing. Centrifuge for 2min at 5,000 rpm.

4. Use 0.5-2 μl flow-through for PCR amplifica

 

50 Preps 250 Preps PCR Sample Preparation Solution P9051 P9052 0

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.

50 Preps 250 Preps PCR Sample Preparation Solution P9051 P9052 1

50 Preps 250 Preps PCR Sample Preparation Solution P9051 P9052 2

Contact Details
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Phone Number : +8618898478663