HIFI Library PCR Master Mix K007-A, K007-B, K007-C

HIFI Library PCR Master Mix K007-A, K007-B, K007-C

Product Details:

Place of Origin: China
Brand Name: GDSBio
Certification: ISO9001, ISO13485
Model Number: K007-A, K007-B, K007-C

Payment & Shipping Terms:

Minimum Order Quantity: 1 box
Packaging Details: small package or bulk distribute or OEM
Delivery Time: 8 work days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 10000 Box/Boxes per Day
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Detail Information

Stock: Yes Cat. No.: K007-A, K007-B, K007-C
Specification: K007-A (40 Rxns); K007-B (400 Rxns); K007-C (2000 Rxns) Application: PCR Amplification Of NGS Libraries
Appearance: Clear Liquid Sequencing Platform: Illumina/MGI
Logo Printing: With Logo Printing Transport Package: Packing
Shelf Life: 24 Months Storage Conditions: Store Unopened At –15°C To –25°C
High Light:

HIFI Library PCR Master Mix

,

PCR Master Mix K007-A

Product Description

HIFI Library PCR Master Mix

【Product Name】

HIFI Library PCR Master Mix

【Cat. No./Spec.】

K007-A (40 rxns); K007-B (400 rxns); K007-C (2000 rxns)

【Product Description】

HIFI Library PCR Master Mix is suitable for PCR amplification of high-throughput sequencing libraries. Contains various components required for PCR reactions (except primers and templates). Using high performance and high fidelity DNA polymerase, special buffer system, efficient amplification while maintaining minimal library bias. The amplification reagent has undergone strict quality control and functional verification to ensure the stability and repeatability of the reagent to the greatest extent.

【Components】

Component K007-A (40 rxns) K007-B (400 rxns) K007-C (2000 rxns)
HIFI Library PCR Master Mix 1 mL × 1 10 mL × 1 10 mL × 5

【Storage Condition & Shelf Life】

Store unopened at –15°C to –25°C until the expiration date on the label. After opening, the master mix may be stored at –15°C to –25°C until the expiration date on the label, or at 4°C for up to 30 days.

【Application】

This product is suitable for PCR amplification of NGS libraries.

【Protocol】

Note: Before setting up the PCR reactions, prepare a primer mix with 20 µM of each primer.

  • Prepare the PCR Reaction Mix

1.1 Allow all reagents to thaw completely. Mix gently by inverting the tube. Spin briefly. Put all reagents on ice.

1.2 The reaction system is formulated according to the following table:

Reagents Volume Final Conc.
HIFI Library PCR Master Mix (2X) 25 µL 1X
Primer mix (20 µM each) 5 µL 2 µM each primer[1]
Template DNA X µL -
Nuclease-free water To 50 µL n/a

 

  • Put the tube into PCR machine for amplification according to the following procedure:
Step Time Temp.(℃)
Hold 3 min 95
2~20 Cycles 20 sec 95
15 sec 60
30 sec 72
Hold 5 min 72
Hold ∞¥ 4

 

【Library Preparation Module】

GDSBio offers the following DNA and RNA library construction modules that can be used in combination for high-quality library preparation:

Module Product Name Cat. No./Spec.
cDNA First Strand Synthesis GDS RNA First Strand Synthesis Module

K020-A/24 rxns

K020-B/96 rxns

Directional cDNA Second Strand Synthesis GDS Directional RNA Second Strand Synthesis Module

K021-A/24 rxns

K021-B/96 rxns

Non-Directional cDNA Second Strand Synthesis GDS Non-Directional RNA Second Strand Synthesis Module

K022-A/20 rxns

K022-B/100 rxns

Fragmentation & End Repair GDS Fragmentation & End Prep Module

K023-A/24 rxns

K023-B/96 rxns

Fragmentation GDS dsDNA Fragmentase

K024-A/50 rxns

K024-B/250 rxns

End Repair/dA-Tailing GDS End Preparation Module

K025-A/24 rxns

K025-B/96 rxns

Adapter Ligation GDS Ligation Module

K026-A/24 rxns

K026-B/96 rxns

Amplification HIFI Library PCR Master Mix

K007-A/40 rxns

K007-B/400 rxns

K007-C/2000 rxns

Cleanup/Size Selection GDSPure DNA Selection Magbeads

NC1011/5 mL

NC1012/60 mL

NC1013/450 mL

 

GDSBio is a high-tech company focused on the development, production and marketing of quality life science products. The company has a complete product line, with PCR technology as the core, focusing on ordinary PCR, fluorescent quantitative PCR, NGS library construction, nucleic acid electrophoresis and other molecular biology technologies, and has developed molecular scientific research reagents, molecular in vitro diagnostic raw materials, nucleic acid extraction and detection reagents and other products.

HIFI Library PCR Master Mix K007-A, K007-B, K007-C 0

 

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