GDS Fragmentation & End Prep Module K023-A , K023-B

GDS Fragmentation & End Prep Module K023-A , K023-B

Product Details:

Place of Origin: China
Brand Name: GDSBio
Certification: ISO9001, ISO13485
Model Number: K023-A, K023-B

Payment & Shipping Terms:

Minimum Order Quantity: 1 bag
Packaging Details: small package or bulk distribute or OEM
Delivery Time: 8 work days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 100 Bag/Bags Per Day
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Detail Information

Cat. No.: K023-A, K023-B Specification: K023-A (24 Rxns); K023-B (96 Rxns)
Application: Fragmentation, End Repair And DA-tailing In DsDNA Library Construction. Stock: Yes
Logo Printing: With Logo Printing Transport Package: Packing
Shelf Life: 12 Months Storage Conditions: Stored At -20°C
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GDS Fragmentation End Prep Module


GDS Fragmentation K023-A

Product Description

GDS Fragmentation & End Prep Module

【Product Name】

GDS Fragmentation & End Prep Module

【Cat. No./Spec.】

K023-A (24 rxns); K023-B (96 rxns)

【Product Description】

GDS Fragmentation & End Prep Module is designed for library construction of high-throughput sequencing platform. The module combines fragmentation of intact DNA, end repair and dA tailing, reducing operation and reaction time and the amount of template. 100 pg ~ 500 ng DNA template can be efficiently fragmented with 5’ phosphorylated 3' dA-tailed ends. This product contains the enzymes and buffers required for the reaction, easy to use, and compatible with automatic library preparation equipment.


Component K023-A (24 rxns) K023-B (96 rxns)
GDS FEP Enzyme Mix 240 μL 2×480 μL
GDS FEP Buffer 120 μL 480 μL
Neutralization Buffer 120 μL 480 μL

【Storage Condition & Shelf Life】

All reagents should be stored at -20°C. The product is valid for 12 months.


Fragmentation, end repair and dA-tailing in dsDNA library construction.


1. Determine the solvent composition of template DNA, if no EDTA, proceed directly to Step 2; If EDTA is contained, 2.2X magnetic beads should be used for purification, or a corresponding volume of Neutralization Buffer should be added according to the content of EDTA in the following table for Neutralization:

EDTA Conc. Volume of Neutralization Buffer
1 mM 5 μL
0.8 mM 4 μL
0.6 mM 3 μL
0.5 mM 2.5 μL
0.4 mM 2 μL
0.2 mM 1 μL
0.1 mM 0.5 μL
<0.1 mM 0 μL

2. Ensure that all reagents are completely thawed. Place on ice until use. Prepare the following reaction in a 200 μL PCR tube:

Reagents Volume
Input DNA X μL
GDS FEP Buffer 5 μL
GDS FEP Enzyme Mix 10 μL
ddH2O To 50 μL

3. Vortex the reaction for 5 seconds and briefly spin in a microcentrifuge.

4. In a thermal cycler, with the heated lid set to 105°C, run the following program:

Temperature Time
37°C 5~30 min *
65°C 30 min
4°C hold

* Use the chart below to determine the incubation time required to generate the desired fragment sizes:

Fragment Size Time
150 bp 20-30 min
250 bp 15-20 min
350 bp 10-15 min
550 bp 6-10 min
  • Adapter ligation directly as soon as possible to avoid excessive DNA fragmentation.

【Library Preparation Module】

GDSBio offers the following DNA and RNA library construction modules that can be used in combination for high-quality library preparation:

Module Product Name Cat. No./Spec.
cDNA First Strand Synthesis GDS RNA First Strand Synthesis Module

K020-A/24 rxns

K020-B/96 rxns

Directional cDNA Second Strand Synthesis GDS Directional RNA Second Strand Synthesis Module

K021-A/24 rxns

K021-B/96 rxns

Non-Directional cDNA Second Strand Synthesis GDS Non-Directional RNA Second Strand Synthesis Module

K022-A/20 rxns

K022-B/100 rxns

Fragmentation & End Repair GDS Fragmentation & End Prep Module

K023-A/24 rxns

K023-B/96 rxns

Fragmentation GDS dsDNA Fragmentase

K024-A/50 rxns

K024-B/250 rxns

End Repair/dA-Tailing GDS End Preparation Module

K025-A/24 rxns

K025-B/96 rxns

Adapter Ligation GDS Ligation Module

K026-A/24 rxns

K026-B/96 rxns

Amplification HIFI Library PCR Master Mix

K007-A/40 rxns

K007-B/400 rxns

K007-C/2000 rxns

Cleanup/Size Selection GDSPure DNA Selection Magbeads

NC1011/5 mL

NC1012/60 mL

NC1013/450 mL


GDS Fragmentation & End Prep Module K023-A , K023-B 0GDSBio is a high-tech company focused on the development, production and marketing of quality life science products. The company has a complete product line, with PCR technology as the core, focusing on ordinary PCR, fluorescent quantitative PCR, NGS library construction, nucleic acid electrophoresis and other molecular biology technologies, and has developed molecular scientific research reagents, molecular in vitro diagnostic raw materials, nucleic acid extraction and detection reagents and other products.

GDS Fragmentation & End Prep Module K023-A , K023-B 1


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