5× RNA One Step Probe RT QPCR Master Mix Colourless Appearance V5011

5× RNA One Step Probe RT QPCR Master Mix Colourless Appearance V5011

Product Details:

Place of Origin: China
Brand Name: GDSBio
Certification: /
Model Number: V5011

Payment & Shipping Terms:

Minimum Order Quantity: 1Bag
Packaging Details: small package or bulk distribute or OEM
Delivery Time: 8 work days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 100 Bag/Bags Per Day
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Detail Information

Cat. No.: V5011 Specification: 200 Rxn 20μl/rxn
Appearance: Colourless Application: RNA Reverse Transcription And Quantitative PCR
Activity: Pass Amplification Capability: Good
Logo Printing: With Logo Printing Transport Package: Packing
Production Capacity: 100 Bag/Bags Per Day Storage Conditions: Store At -20°C
High Light:

One Step RT QPCR Master Mix V5011

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Colourless One Step RT QPCR Master Mix

Product Description

One-step Probe RT-qPCR Kit V3

High Quality 5× One-Step Probe RT-QPCR Kit V3 V5011

Cat. No.:V5011

Components

Component V5011 (200 rxn, 20 μl/rxn)
5 × One-step U+ Mixa 800 µl
One-step U+ Enzyme Mix V3b 200 µl

a. Contains dNTP/dUTP Mix, Mg2+.

b. Contains Reverse Transcriptase, RNase inhibitor, Heat-labile UDG and hot-start Taq DNA Polymerase.

 

Description

The One-step Probe RT-qPCR Kit V3 is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55°C, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Integrating the superior performance of Reverse Transcriptase and hot-start Taq DNA Polymerase, with the optimized buffer system, the detection sensitivity of One-step Probe RT-qPCR Kit can reach 0.1 pg total RNA or <10 copies of RNA template. The kit is available as a convenient Master Mix. 5 × One-step U+ Mix contains an optimized buffer system and dNTP/dUTP Mix, which is suitable for high-specificity, low template concentration and multiple detection systems with fluorescent labeled probes such as TaqMan®. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.

 

Storage

This reagent should be kept at -20°C.

 

Applications

• Probe gene expression analysis

• Probe Low-copy gene detection

• Probe microarray validation

• Probe gene knockdown validation

 

Features

• One-step RT-qPCR

• This kit is suitable for fluorescence quantification by probe method

• This kit is compatible with many real-time systems

• Hot-start technology brings high specificity and reproducible amplification

• dUTP/UDG system, effectively prevent PCR product contamination

 

Notes

1. One-Step U+ Enzyme Mix V3 contains high concentration of glycerol. Before use, please centrifuge briefly, collect to the bottom of the reaction tube, and gently suck with a pipetting gun.

2. For preparation of reaction solution, please use RNase-free pipette tips, EP tubes, etc., to avoid contamination as far as possible.

 

 

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GuangZhou Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.

 

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