• T4 Polynucleotide Kinase T4 PNK #K013 Logo Printing
T4 Polynucleotide Kinase T4 PNK #K013 Logo Printing

T4 Polynucleotide Kinase T4 PNK #K013 Logo Printing

Product Details:

Place of Origin: China
Brand Name: GDSBio
Certification: /
Model Number: K013

Payment & Shipping Terms:

Minimum Order Quantity: 500U
Price: inquiry
Packaging Details: small package or bulk distribute or OEM
Delivery Time: 8 work days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 100 Bag/Bags Per Day
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Detail Information

Cat. No.: K013-A Specification: 500U
Appearance: Colourless Application: 5' Phosphorylation Of DNA/RNA For Subsequent Ligation
Classification: General Reagents Grade: Molecular Biology
Logo Printing: With Logo Printing Transport Package: Packing
Production Capacity: 100 Bag/Bags Per Day Storage Conditions: Store At -20°C
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T4 Polynucleotide Kinase


PNK T4 Polynucleotide Kinase

Product Description

Cat. No./Spec.: K013-A/500U; K013-B/2500U; K013-C/10000U

Concentration: 10 U/µL


Product Description

T4 Polynucleotide Kinase (T4 PNK) catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5'-hydroxyl terminus of polynucleotides (double-stranded and single-stranded DNA and RNA) and nucleoside 3'-monophosphate. T4 polynucleotide kinase also catalyzes the removal of 3′-phosphoryl groups from 3′-phosphate-based polynucleotides, deoxynucleoside 3′-monophosphate, and deoxynucleoside 3′-diphosphate. When ADP is present, T4 PNK has 5'-end phosphatase activity, catalyzing the exchange of 5'-P-oligomeric/polymeric nucleotides and ATP terminal 5'-phosphate groups. It is suitable for constructing DNA libraries and producing probes with terminal labeling.





(500 U)


(2,500 U)


(10,000 U)

T4 Polynucleotide Kinase (10 U/µL) 50 µL 250 µL 1 mL
10X T4 PNK Buffer * 500 µL 1 mL 1 mL × 2

* 10 × T4 PNK Buffer does not contain ATP, and customers need to add it themselves, with a final concentration of 1 mM, or use T4 DNA ligase buffer.


Storage Condition & Shelf Life

All reagents should be stored at -20°C. The product is valid for 12 months.


Unit Definition

One Richardson unit is defined as the amount of enzyme catalyzing the recombination of 1 nmol of [γ-32P] ATP.


Scope of Application

1. End labeling DNA or RNA for probes and DNA sequencing;

2. 5' phosphorylation of DNA/RNA for subsequent ligation;

3. Phosphorylate the 5´ end of the 3´ phosphorylated mononucleotide to prepare the pNp substrate for addition to the 3´ end of DNA or RNA;

4. Label the 5´ end of oligonucleotides with a phosphate group at the 3´ end.

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