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DNase I, RNase-Free Molecular Tool Enzyme
Product Details:
| Place of Origin: | China |
| Brand Name: | GDSBio |
| Certification: | / |
| Model Number: | E1018 |
Payment & Shipping Terms:
| Minimum Order Quantity: | 1000 U |
|---|---|
| Price: | USD 76-84.5/1000 U |
| Packaging Details: | small package or bulk distribute or OEM |
| Delivery Time: | 8 work days |
| Payment Terms: | L/C, D/A, D/P, T/T, Western Union, MoneyGram |
| Supply Ability: | 100 Bag/Bags Per Day |
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Detail Information |
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| Product Name: | DNase I, RNase-Free, HC | Cat. No./Spec.: | E1018-A/1,000 U |
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| Appearance: | Colourless | Application: | Cleave Both Single-stranded And Double-stranded DNA |
| Classification: | General Reagents | Grade: | Molecular Biology |
| Logo Printing: | With Logo Printing | Transport Package: | Packing |
| Production Capacity: | 100 Bag/Bags Per Day | Storage Conditions: | Store At -20°C |
| High Light: | Colourless Molecular Tool Enzyme,RNase-Free Molecular Tool Enzyme |
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Product Description
DNase I, RNase-Free, HC
Cat. No./Spec.: E1018-A/1,000 U
Concentration: 50 U/µL
Product Description
DNase I (without RNase) is an endonuclease that can cleave both single-stranded and double-stranded DNA. It hydrolyzes
phosphodiester bonds, producing single deoxyribonucleotides and oligodeoxyribonucleotides with 5'-phosphate groups and 3'-OH groups.
The activity of this enzyme is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions. In the presence of Mg2+, DNase I cuts each strand of dsDNA in a statistically random and independent manner. When Mn2+ is present, the enzyme almost cuts both DNA strands at the same site, resulting in DNA fragments with blunt ends or overhangs of one or a few nucleotides.
Cat. No./Spec.: E1018-A/1,000 U
Concentration: 50 U/µL
Product Description
DNase I (without RNase) is an endonuclease that can cleave both single-stranded and double-stranded DNA. It hydrolyzes
phosphodiester bonds, producing single deoxyribonucleotides and oligodeoxyribonucleotides with 5'-phosphate groups and 3'-OH groups.
The activity of this enzyme is strictly dependent on Ca2+ and is activated by Mg2+ or Mn2+ ions. In the presence of Mg2+, DNase I cuts each strand of dsDNA in a statistically random and independent manner. When Mn2+ is present, the enzyme almost cuts both DNA strands at the same site, resulting in DNA fragments with blunt ends or overhangs of one or a few nucleotides.
Storage Condition & Shelf Life
Store at -20°C.
Source
Recombinant E. coli strain containing the cloned gene encoding bovine DNase I.
Unit Definition
A unit is defined as the amount of enzyme required to completely degrade 1 µg of plasmid DNA within 1 minute at 37°C.
Enzyme activity is determined in the following mixture: 10 mM Tris-HCl (pH 7.5 at 25°C), 2.5 mM MgCl2, 0.1 mM CaCl2, and 1 µg of pUC19 DNA.
One unit of DNase I is equivalent to 0.3 Kunitz units.
Features
- Recombinant enzyme
- Purified from a non-animal host, with low levels of endogenous RNase
Scope of Application
- For the preparation of DNA-free RNA.
- To remove template DNA after in vitro transcription.
- To prepare DNA-free RNA before RT-PCR and RT-qPCR.
- In conjunction with DNA Polymerase I for DNA labeling through nick translation.
- For the study of DNA-protein interactions using DNase I (RNase-free) footprinting.
- To generate a library of randomly sheared DNA insert fragments. Reaction buffer containing Mn2+ is used.
Store at -20°C.
Source
Recombinant E. coli strain containing the cloned gene encoding bovine DNase I.
Unit Definition
A unit is defined as the amount of enzyme required to completely degrade 1 µg of plasmid DNA within 1 minute at 37°C.
Enzyme activity is determined in the following mixture: 10 mM Tris-HCl (pH 7.5 at 25°C), 2.5 mM MgCl2, 0.1 mM CaCl2, and 1 µg of pUC19 DNA.
One unit of DNase I is equivalent to 0.3 Kunitz units.
Features
- Recombinant enzyme
- Purified from a non-animal host, with low levels of endogenous RNase
Scope of Application
- For the preparation of DNA-free RNA.
- To remove template DNA after in vitro transcription.
- To prepare DNA-free RNA before RT-PCR and RT-qPCR.
- In conjunction with DNA Polymerase I for DNA labeling through nick translation.
- For the study of DNA-protein interactions using DNase I (RNase-free) footprinting.
- To generate a library of randomly sheared DNA insert fragments. Reaction buffer containing Mn2+ is used.
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