• Fast Restriction Endonuclease NcoI
Fast Restriction Endonuclease NcoI

Fast Restriction Endonuclease NcoI

Product Details:

Place of Origin: China
Brand Name: GDSBio
Certification: /
Model Number: E1026

Payment & Shipping Terms:

Minimum Order Quantity: 20 rxns
Price: USD 20.2-66/20 rxns
Packaging Details: small package or bulk distribute or OEM
Delivery Time: 8 work days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 100 Bag/Bags Per Day
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Detail Information

Product Name: NcoI Cat. No./Spec.: E1026-A/20 Rxns, E1026-B/100 Rxns
Appearance: Colourless Application: Molecular Cloning
Classification: General Reagents Grade: Molecular Biology
Logo Printing: With Logo Printing Transport Package: Packing
Production Capacity: 100 Bag/Bags Per Day Storage Conditions: Store At -20°C
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fast restriction endonuclease NcoI


Endonuclease NcoI for Molecular Cloning

Product Description


Cat. No./Spec.: E1026-A/20 rxns, E1026-B/100 rxns
Recognition site:
5' C ↓ C A T G G 3'
3' G G T A C ↑ C 5'

Product Description
The NcoⅠ restriction endonuclease recognizes the C^CATGG site and achieves optimal cutting efficiency within 5–15 minutes at 37°C using the universal buffer.

GDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.
The universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.
The Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.
Storage Condition & Shelf Life
Store at -20°C.

- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.
- 100% buffer compatibility with downstream applications.
- Enzymatic digestion can be completed within 5–15 minutes.
- Direct loading onto a gel.
- No star activity.

Scope of Application
- Molecular cloning
- Restriction site mapping
- Genotyping
- Southern blotting
- Restriction fragment length polymorphism (RFLP)
- SNP (Single Nucleotide Polymorphism) analysis

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