1STEP Universal DNA Library Prep Kit

1STEP Universal DNA Library Prep Kit is a universal enzyme-based DNA library preparation kit, suitable for high-throughput sequencing platforms such as Illumina and MGI. This kit integrates DNA fragmentation, end repair, and dA tailing in one step. The products do not require purification and can be directly used for adapter ligation, simplifying the workflow and reducing the library preparation time to under 2 hours. The kit uses a hot-start high-fidelity DNA polymerase for library amplification, compatible with DNA samples ranging from 1 ng to 500 ng. It also supports PCR-free library construction.

【Application Scope】
For DNA library preparation for high-throughput sequencing, including whole genome sequencing, metagenomic sequencing, exome sequencing, and targeted sequencing. The kit is compatible with DNA input amounts ranging from 1 ng to 500 ng.
【Key Features】
•One-step library preparation: DNA fragmentation, end repair, and dA tailing are performed in a single tube, eliminating the need for intermediate purification.
•Compatible with all major sequencing platforms: Directly adapted for Illumina, MGI, and other high-throughput sequencing platforms.
•Wide sample compatibility: Compatible with 1 ng to 500 ng DNA from various sources, ensuring high transformation efficiency.
•High-fidelity and low background: The kit uses a hot-start high-fidelity enzyme to suppress non-specific amplification, minimizing background contamination.
•Flexible PCR-free option: Allows for PCR-free library preparation, avoiding amplification biases and ensuring uniform coverage.