GDS dsDNA Fragmentation Enzyme

GDS dsDNA Fragmentation Enzyme, a double-stranded DNA fragmentation enzyme, produces dsDNA breaks in a time-dependent manner, producing DNA fragments of 50-1,000 bp depending on reaction time. The GDS dsDNA Fragmentation Enzyme consists of two enzymes, one that randomly generates nicks on dsDNA and the other recognizes the nicked sites and cuts the opposite DNA strand aross from the nick, thus producing dsDNA breaks. The resulting DNA fragment contains short overhangs, 5' -phosphate, and 3' -hydroxyl groups. The random nicking activity of GDS dsDNA Fragmentation Enzyme has been demonstrated by the preparation of next-generation sequencing libraries. The comparison of the sequencing results between libraries prepared by genomic DNA sheared by GDS dsDNA Fragmentation Enzyme with mechanically shearing showed that GDS dsDNA Fragmentation Enzyme does not introduce any detectable bias in the preparation of sequencing libraries, and there is no difference in sequence coverage between the two methods.

Application
Fragmentation of dsDNA.

Features
Random fragmentation can be achieved by adjusting the incubation time