GDSlyo Fast One-step Probe RT-qPCR Kit

GDSlyo Fast One-step Probe RT-qPCR Kit is a one-step probe-based RT-qPCR reagent optimized for the lyophilization process. It employs an optimized combination of a genetically engineered fast thermostable reverse transcriptase and a high-fidelity hot-start DNA polymerase, coupled with a highly efficient reaction buffer system, enabling complete amplification in just 20-40 minutes, significantly improving detection throughput. The included thermostable reverse transcriptase is engineered to remove RNase H activity, offering higher cDNA synthesis efficiency and amplification sensitivity, making it particularly suitable for the stable detection of low-concentration RNA templates.

This kit produces excellent standard curves over a wide linear range, ensuring accurate and reliable quantification. It is also compatible with various fast PCR instruments and rapid amplification programs, meeting the needs of high-throughput and rapid testing applications. The built-in optimized qPCR buffer and dUTP/temperature-sensitive UDG contamination prevention system effectively eliminate false positives caused by aerosols and product carryover. The lyophilized product has good formability and excellent stability, suitable for ambient temperature storage and transportation and various molecular diagnostic scenarios.

Applications
Preparation of lyophilized RT-qPCR reagents.
IVD product development for RNA detection (e.g., virus samples).
One-step RT-qPCR experiments for multiple RNA targets.

Features

Fast lyophilized RT-qPCR kit: Designed for rapid detection with short RT and amplification times.

Effective lyoprotection: Maintains enzyme activity and stability after lyophilization.

High inhibitor tolerance: Suitable for direct amplification from crude samples.

Dual cycling protocols: Offers both standard and fast programs for flexibility.

Ideal for IVD and POCT: Suitable for developing rapid diagnostic tests and point-of-care applications.