One-step Probe RT-qPCR Kit V2

One-step Probe RT-qPCR Kit V2 is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55¡ãC, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Thermostable reverse transcriptase can maintain stable activity at 55¡ãC. The hot-start antibody-modified fast DNA polymerase and optimized buffer system ensure the extremely high sensitivity and specificity of the One-Step Probe RT-qPCR Kit V2. The kit is available as a convenient Master Mix. 2X RT-qPCR Mix contains an optimized buffer system and dNTP/dUTP Mix, which is suitable for high-specificity detection systems with fluorescent labeled probes such as TaqMan.

Application
RNA virus & pathogen detection – e.g., SARS-CoV-2, HIV, influenza, dengue, HCV
Gene expression quantification – from low-abundance or precious RNA samples
Multiplex target detection – simultaneous detection of 4–6 targets in one tube
Direct amplification (no RNA extraction) – using crude lysates from swabs, blood, tissues
Low-abundance & high-GC content RNA templates – high sensitivity down to a few copies or 0.1 pg total RNA

Features
Fast start-up, 30s hot start
Reverse transcription at 55 ¡ãC to accommodate complex RNA templates
Contains heat-labile UDG to reduce false-positive contamination
High sensitivity for detection of low-abundance genes
High stability, unchanged performance at 4¡ãC and 37¡ãC for one week
The reaction system can be formulated up to 30 minutes in advance without changing performance
Saves time by supporting fast program