One-step Probe RT-qPCR Kit V3

One-step Probe RT-qPCR Kit V3 is designed for quantitative PCR detection using RNA as a template, such as viral RNA. Using gene-specific primers (GSP), reverse transcription and qPCR reactions are performed in a single tube, requiring no additional tube opening/pipetting, significantly increasing detection throughput and reducing the risk of contamination. This Kit introduced dUTP/UDG system. Heat-labile UDG can rapidly degrade the pollutants containing U at room temperature. When reverse transcription is performed at 55℃, heat-labile UDG will be inactivated rapidly without affecting the efficiency and sensitivity of RT-qPCR. Integrating the superior performance of Reverse Transcriptase and hot-start Taq DNA Polymerase, with the optimized buffer system, the detection sensitivity of One-step Probe RT-qPCR Kit can reach 0.1 pg total RNA or <10 copies of RNA template. The kit is available as a convenient Master Mix. 5X One-step U+ Mix contains an optimized buffer system and dNTP/dUTP Mix, which is suitable for high-specificity, low template concentration and multiple detection systems with fluorescent labeled probes such as TaqMan. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.

Application
RNA virus & pathogen detection – e.g., SARS-CoV-2, HIV, influenza, dengue, HCV
Gene expression quantification – from low-abundance or precious RNA samples
Multiplex target detection – simultaneous detection of 4–6 targets in one tube
Direct amplification (no RNA extraction) – using crude lysates from swabs, blood, tissues
Low-abundance & high-GC content RNA templates – high sensitivity down to a few copies or 0.1 pg total RNA

Features
Fast start-up, 30s hot start
Reverse transcription at 55℃ to accommodate complex RNA templates
Contains heat-labile UDG to reduce false-positive contamination
High sensitivity for detection of low-abundance genes
High stability, unchanged performance at 4℃ and 37℃ for one week
The reaction system can be formulated up to 30 minutes in advance without changing performance
Saves time by supporting fast program