One-step Probe RT-qPCR Kit V4

One-step Probe RT-qPCR Kit V4 is a high-performance one-step probe-based reverse transcription quantitative PCR (RT-qPCR) master mix, consisting of two components: an enzyme mix and a reaction buffer. This product employs an optimized combination of antibody-modified hot-start Taq DNA polymerase and highly thermostable Power Reverse Transcriptase, coupled with a deeply optimized reaction buffer system, to achieve highly specific and sensitive detection results. The product offers excellent stability at ambient temperature, facilitating transportation and storage, and significantly improving user convenience. The dUTP/temperature-sensitive UDG contamination prevention system effectively prevents false positives caused by PCR product carryover and aerosol contamination, ensuring the accuracy and reliability of results. It is suitable for developing various high-sensitivity RNA detection kits.

Application
RNA virus & pathogen detection – e.g., SARS-CoV-2, HIV, influenza, dengue, HCV
Gene expression quantification – from low-abundance or precious RNA samples
Multiplex target detection – simultaneous detection of 4–6 targets in one tube
Direct amplification (no RNA extraction) – using crude lysates from swabs, blood, tissues
Low-abundance & high-GC content RNA templates – high sensitivity down to a few copies or 0.1 pg total RNA

Features

High-performance enzyme mix: Antibody-modified Taq and stable reverse transcriptase ensure high specificity and sensitivity.

Optimized buffer system: Enhances reaction efficiency and storage stability.

dUTP/UDG anti-contamination system: Effectively prevents false positives from amplicon carryover.

Broad applicability: Suitable for multiplex detection and high-sensitivity RNA assay development.

Excellent amplification efficiency: Supports long amplicons (>200 bp) and low-efficiency primers; compatible with three-step cycling.