Optimus Hotstart Taq Mix

Optimus Hotstart Taq Mix is a premixed, ready-to-use solution containing Hotstart Taq DNA Polymerase, dNTPs, Mg2+ and Reaction Buffer at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. This premixed formulation saves time and reduces contamination due to the fewer pipetting steps required for PCR set-up. The mix retains all features of Hotstart Taq DNA Polymerase.

Nucleic acid‑free version available. Optimus Hotstart Taq Mix (NAF) P2041F/P2042F/P2043F/P2044F

Application
High-specificity amplification & multiplex PCR

Features
High specificity
hotstart with antibody modification
Elongation speed: 1kb/min
generates 3'-dA overhangs

The PCR master mix, formulated with an upgraded hot‑start Taq polymerase as the core component, not only delivers substantially improved amplification efficiency but also ensures high specificity.

A comparative study was conducted between the upgraded hot‑start polymerase master mix (P2041), the original master mix (P2041), and the non‑hot‑start master mix (P2021). The results demonstrated that the upgrade in hot‑start performance not only resolved the issue of low amplification efficiency observed in the original formulation, but also maintained exceptionally high specificity.