Proteinase K (recombinant), PCR grade

Concentration: ≥ 600 U/mL (~20 mg/mL)

Product Description
Proteinase K is an endopeptidase capable of hydrolyzing peptide bonds at the carboxyl terminus of aliphatic, aromatic, or hydrophobic amino acids. Proteinase K belongs to the family of serine proteases. This enzyme can minimally hydrolyze tetra-peptides.

Source
Recombinant Pichia pastoris strain containing a cloned gene from Tritirachium album.

Unit Definition

A unit is defined as the amount of enzyme required to release an amount of Folin-positive amino acids and peptides equivalent to 1 µmol of tyrosine from denatured hemoglobin in 1 minute at 37°C.

Enzyme activity is determined in the following mixture: 0.08M potassium phosphate (pH 7.5), 5M urea, 4mM NaCl, 3mM CaCl₂, and 16.7 mg/mL hemoglobin.

Features
- Ready-to-use solution
- Compatible with a wide range of reaction conditions

Scope of Application
- Isolate genomic DNA from mouse tails.
- Isolate genomic DNA from cultured cells.
- Eliminate DNase and RNase during the isolation of DNA and RNA from tissues or cell lines.
- Determine the location of enzymes.
- Improve the cloning efficiency of PCR products.