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GDSBIO
SacI
SacI
Prix habituel
$61.00 USD
Prix habituel
Prix promotionnel
$61.00 USD
Prix unitaire
/
par
Frais d'expédition calculés à l'étape de paiement.
Impossible de charger la disponibilité du service de retrait
Recognition site:
5' G A G C T ↓ C 3'
3' C ↑ T C G A G 5'
Product Description
The SacⅠ restriction endonuclease recognizes the GAGCT^C site and achieves optimal cutting efficiency within 5–15 minutes at 37°C using the universal buffer. Isoschizomers: Eco53kⅠ, EcoICRⅠ, Psp124BⅠ, SstⅠ.
GDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.
The universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.
The Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.
5' G A G C T ↓ C 3'
3' C ↑ T C G A G 5'
Product Description
The SacⅠ restriction endonuclease recognizes the GAGCT^C site and achieves optimal cutting efficiency within 5–15 minutes at 37°C using the universal buffer. Isoschizomers: Eco53kⅠ, EcoICRⅠ, Psp124BⅠ, SstⅠ.
GDSBio restriction endonucleases exhibit 100% activity in both Digest and Green reaction buffers.
The universal Digest buffer allows for rapid single-enzyme, double-enzyme, or multiple-enzyme digestion of DNA within 5–15 minutes, eliminating the need for buffer exchange or subsequent DNA purification steps. DNA-modifying enzymes (such as the Klenow fragment, T4 DNA ligase, calf intestinal alkaline phosphatase, and T4 DNA polymerase) maintain 100% activity in the buffer. Consequently, enzymes used in downstream applications can be directly added to the reaction mixture. Shorter incubation times and the superior composition of the universal Digest buffer eliminate the star activity effect.
The Green buffer includes one density reagent and two tracking dyes for direct loading of the digestion products onto a gel.
Storage Condition
Store at -20°C.
Features
- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.
- 100% buffer compatibility with downstream applications.
- Enzymatic digestion can be completed within 5–15 minutes.
- Direct loading onto a gel.
- No star activity.
Scope of Application
- Molecular cloning
- Restriction site mapping
- Genotyping
- Southern blotting
- Restriction fragment length polymorphism (RFLP)
- SNP (Single Nucleotide Polymorphism) analysis
Store at -20°C.
Features
- All GDSBio restriction endonucleases exhibit 100% activity in the universal buffer.
- 100% buffer compatibility with downstream applications.
- Enzymatic digestion can be completed within 5–15 minutes.
- Direct loading onto a gel.
- No star activity.
Scope of Application
- Molecular cloning
- Restriction site mapping
- Genotyping
- Southern blotting
- Restriction fragment length polymorphism (RFLP)
- SNP (Single Nucleotide Polymorphism) analysis
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Rappels de prévente
1. Demande par e-mail
Pour obtenir des informations détaillées, contactez notre équipe commerciale ou envoyez un courriel à order@gdsbio.com (ou remplissez le formulaire ci-dessus).
2. Demande par service client
Utilisez la fenêtre de chat dans le coin inférieur droit (assistant IA) pour laisser votre question et vos coordonnées. Nous vous répondrons dans les plus brefs délais.
3. Expédition et logistique
Tous les produits sont expédiés depuis Guangzhou, avec un emballage anglais par défaut.
- La plupart des articles sont en stock ; certains peuvent prendre quelques jours pour être préparés. Nous expédions dès qu’ils sont prêts.
- Les produits nécessitent généralement une expédition à basse température (boîte en mousse + packs de glace), ce qui entraîne des frais de port plus élevés.
- Transporteur principal : FedEx, DHL.