Super 4X One-Step Multiplex Master Mix

Super 4X One-Step Multiplex Master Mix is used to perform one-step multiplex real-time PCR applications with any gene-specific primer and probe sets, and is suitable for both RNA and DNA targets. Dual hot-start reverse transcriptase and DNA polymerase can complete more than 10-plex highly sensitive and specific quantitative PCR in a single system. The master mix is supplied at a 4X concentration that allows to input more sample into each reaction, increasing sensitivity even in low-volume reactions.

Application
Ultra‑multiplex pathogen detection – Detects >10 RNA/DNA targets in one tube (e.g., respiratory viruses, bloodborne pathogens).
High sensitivity with low‑abundance targets – 4X concentration allows more sample input, ideal for liquid biopsy, low‑copy viruses, or precious specimens.
Gene expression & biomarker panels – Simultaneously quantifies multiple mRNAs (e.g., cytokines, oncogenes) from limited RNA samples.
Small‑volume & high‑throughput qPCR – Works in 5‑10 µL reactions, suitable for 384‑well plates, microfluidics, or portable qPCR devices.
Direct amplification from crude samples – Tolerates inhibitors, enabling one‑step testing from swabs, blood lysates, or environmental samples without RNA extraction.

Features
support 10-plex amplification with high specificity
Reverse transcription at 55 ¡ãC to accommodate complex RNA templates
Contains heat-labile UDG to reduce false-positive contamination
High sensitivity for detection of low-abundance genes
High stability
4X formation, high concentration
Saves time by supporting fast program