T7 RNA Polymerase

Concentration: 20 U/µL

Product Description
Phage T7 RNA polymerase is a DNA-dependent RNA polymerase that has strict specificity for its corresponding double-stranded promoter. It catalyzes the synthesis of RNA in the 5'→3' direction downstream of the promoter, starting from the promoter on single-stranded DNA or double-stranded DNA.

Source
Recombinant E. coli strain containing the cloned gene encoding this enzyme.

Unit Definition
A unit is defined as the amount of enzyme required to incorporate 1 nmol of AMP into a polynucleotide substrate within 60 minutes at 37°C.

Features
- Capable of incorporating modified nucleotides (such as aminoallyl, biotin, fluorescein, digoxigenin-labeled nucleotides).

Scope of Application
The synthesis of unlabeled and labeled RNA can be used for:
Hybridization, in vitro RNA translation
As a substrate in aRNA, siRNA, RNase protection assays, and as a template for genomic DNA sequencing
RNA splicing in studies of RNA secondary structure and RNA-protein interactions.