FS PCR Master Mix PCR Kit For DNA RNA Nucleic Acid Detection P3072 1ml×5
Product Details:
Place of Origin: | China |
Brand Name: | GDSBio |
Certification: | / |
Model Number: | P3072 |
Payment & Shipping Terms:
Minimum Order Quantity: | 1Bag |
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Packaging Details: | small package or bulk distribute or OEM |
Delivery Time: | 8 work days |
Payment Terms: | L/C, D/A, D/P, T/T, Western Union, MoneyGram |
Supply Ability: | 100 Bag/Bags Per Day |
Detail Information |
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Cat. No.: | P3072 | Specification: | P3072 1ml×5 |
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Appearance: | Blue Buffer | Group: | PCR Master Mix |
Activity: | Pass | Amplification Capability: | Good |
Logo Printing: | With Logo Printing | Transport Package: | Packing |
Production Capacity: | 100 Bag/Bags Per Day | Storage Conditions: | Store At -20°C |
High Light: | FS PCR Master Mix,PCR Master Mix DNA RNA Nucleic Acid Detection,1ml pcr kit |
Product Description
FSTM Kit
#P3072, 5 ml
Contents:
2× FSTM Reaction Mix | 1 ml × 5 |
FSTM Taq DNA Polymerase (2.5U/μl) | 160 μl |
Description
FSTM Kit contains FSTM Taq DNA Polymerase and a premixed, ready-to-use FSTM Reaction Mix, which contains dNTPs, Mg2+ and other components at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. The reaction Mix has higher amplification sensitivity due to optimized components and enhancer.
FSTM Taq DNA Polymerase is the latest generation Taq-based DNA polymerase developed by GDSBio. It possesses high amplification efficiency as Taq DNA Polymerase does, and fast elongation ability as KOD polymerase does, can be used in various kinds of PCR. The FSTM PCR Buffer is designed for FSTM Taq DNA Polymerase, can be used in fast amplification reaction. The elongation rate of FSTM Taq DNA Polymerase can be as fast as 3kb/min. It can shorten the amplification time by 2/3. It has 5' to 3' polymerase activity, but lacks of 3' to 5' exonuclease activity, that results in a 3'-dA overhangs PCR product.
Unit Definition
One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.
Storage Buffer
20mM Tris-HCl (pH8.0), 1mM DTT, 0.1mM EDTA, 3.2mM MgCl2, 100mM KCl, 0.1% Triton X-100, 0.1% TW 20, 0.2mg/ml BSA, 50% Glycerol
Composition of the 2× FSTM Reaction Mix
2× FSTM Buffer, 0.4mM dNTPs, 3.2mM MgSO4, 0.02% bromophenol blue.
FSTM Reaction Mix is a proprietary formulation optimized for robust performance in PCR.
Applications
• High throughput PCR
• Long and complex template PCR
• Routine PCR with high reproducibility
• Generation of PCR products for TA cloning
Features
• Convenient: only primers and template DNA are added
when prepare final PCR
• High yields of PCR products with minimal optimization
• High efficiency: saving your time by simplifying the process
• Reproducible: lower contamination and pipetting error risk
• Higher sensitivity and fast compared to conventional Taq DNA Polymerase
• Flexible: the amount of polymerase is flexible and controllable.
Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.