|Place of Origin:||China|
|Minimum Order Quantity:||1Bag|
|Packaging Details:||small package or bulk distribute or OEM|
|Delivery Time:||8 work days|
|Payment Terms:||L/C, D/A, D/P, T/T, Western Union, MoneyGram|
|Supply Ability:||100 Bag/Bags Per Day|
|Cat. No.:||P2091a/P2092a||Specification:||P2091a, 400 Rxn/20μl Reaction； P2092a, 2000 Rxn/20μl Reaction|
|Appearance:||Colourless||Group:||Real Time PCR Mix|
|Logo Printing:||With Logo Printing||Transport Package:||Packing|
|Production Capacity:||100 Bag/Bags Per Day||Storage Conditions:||Store At -20°C|
SYBR Green qpcr mastermix,
qpcr mastermix Low ROX+,
Real Time pcr reagent mix
Cat. No: P2091a, 400 rxn/20μl reaction
P2092a, 2000 rxn/20μl reaction
SYBR® Green qPCR Mix (Low ROX+)
SYBR® Green qPCR Mix (Low ROX+) P2091a/P2092a
For research use only
|2X SYBR® Green qPCR Mix (Low ROX+)||1 ml||1 ml × 5|
|Nuclease-free Water||1 ml||1 ml × 5|
This reagent can be stored for 2 months at 4°C and protected from light. For longer storage, it should be kept at -20°C and protected from light.
SYBR® Green qPCR Mix (Low ROX+) is designed for high-performance, high-throughput real-time PCR. The kit contains Taq DNA Polymerase engineered through a process of molecular evolution. The result is a unique polymerase that specifically designed for qPCR using SYBR® Green I chemistry dye.
2X SYBR® Green qPCR Mix (Low ROX+) is a convenient premix of the components (except primers and DNA template) that necessary to perform real-time polymerase chain reaction (PCR) using SYBR® Green I dye with enhanced sensitivity and specificity. The SYBR® Green I dye binds to double-stranded DNA (dsDNA), thus providing a fluorescent signal that reflects the amount of dsDNA products generated during PCR.
• Gene expression analysis
• Low-copy gene detection
• Microarray validation
• Gene knockdown validation
• Compatible with many Real-time systems which requires low concentrtion of ROX reference dye to calibration
• Hot-start technology brings high specificity and reproducible amplification
Composition of the 2X SYBR Green qPCR Mix (Low ROX+)
100mM KCl , 5mM MgCl2, 400μM dNTPs, 0.1U/μl Hotstart Taq DNA Polymerase, 1× SYBR® Green I, 0.1μM ROX reference dye and other optimized buffer components.
Table of Instrument Guide
|Instruments||Final Conc. of ROX|
|ABI PRISM 7000/ PRISM 7700/ 7300/ 7900HT/ StepOne/ StepOnePlus/ GeneAmp 5700||500 nM,high ROX|
|ABI 7500/ 7500 Fast/ ViiA 7/ QuantStudio 6/7/12K Flex; Agilent Stratagene Mx3000P/ Mx3005P/ Mx4000||50 nM,low ROX|
|Bio-Rad CFX96/ CFX384/ iQ/ iQ5; MJ Research Opticon 2/ Chromo 4; Roche LightCycler 480/ 96; Corbett Rotor Gene G/ Q/ 3000/ 6000; Thermo PikoReal 96; Eppendorf MasterCycler ep realplex; Cepheid Smart Cycler||No ROX|
Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.