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Real Time SYBR Green QPCR Master Mix With ROX P2091c P2092c

Basic Information
Place of Origin: China
Brand Name: GDSBio
Certification: /
Model Number: P2091c/P2092c
Minimum Order Quantity: 1Bag
Packaging Details: small package or bulk distribute or OEM
Delivery Time: 8 work days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 100 Bag/Bags Per Day
Detail Information
Cat. No.: P2091c/P2092c Specification: P2091c, 400 Rxn/20μl Reaction P2092c, 2000 Rxn/20μl Reaction
Appearance: Colourless Group: Real Time PCR Mix
Activity: Pass Amplification Capability: Good
Logo Printing: With Logo Printing Transport Package: Packing
Production Capacity: 100 Bag/Bags Per Day Storage Conditions: Store At -20°C
High Light:

Real Time sybr green qpcr master mix

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sybr green qpcr master mix ROX

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400 rxn sybr green qpcr mix


Product Description

Cat. No: P2091c, 400 rxn/20μl reaction

P2092c, 2000 rxn/20μl reaction

 

SYBR® Green qPCR Mix with ROX

SYBR® Green qPCR Mix with ROX P2091c/P2092c

For research use only

Components

Component P2091c P2092c
2X SYBR® Green qPCR Mix 1 ml 1 ml × 5
50X ROX Reference Dye H* 40 μl 200 μl
50X ROX Reference Dye L* 40 μl 200 μl
Nuclease-free Water 1 ml 1 ml × 5

* ROX Reference Dye H and ROX Reference Dye L are suitable for fluorescence quantitative PCR instruments requiring high and low concentrations of ROX calibration respectively. It is recommended that 40 μL 50X ROX Reference Dye H or L be premixed into 1ml 2X SYBR Green qPCR Mix. The final working concentration of ROX is 1X.

 

Storage

This mix can be stored for 2 months at 4°C and protected from light. For longer storage, it should be kept at -20°C and protected from light. The ROX Reference Dye should be kept at -20°C and protected from light.

 

Description

SYBR® Green qPCR Mix with ROX is designed for high-performance, high-throughput real-time PCR. The kit contains Taq DNA Polymerase engineered through a process of molecular evolution. The result is a unique polymerase that specifically designed for qPCR using SYBR® Green I chemistry dye.

2X SYBR® Green qPCR Mix with ROX is a convenient premix of the components (except primers and DNA template) that necessary to perform real-time polymerase chain reaction (PCR) using SYBR® Green I dye with enhanced sensitivity and specificity. The SYBR® Green I dye binds to double-stranded DNA (dsDNA), thus providing a fluorescent signal that reflects the amount of dsDNA products generated during PCR.

 

Applications

• Gene expression analysis

• Low-copy gene detection

• Microarray validation

• Gene knockdown validation

 

Features

• Compatible with many Real-time systems whether requires ROX reference dye to calibration or not

• Hot-start technology brings high specificity and reproducible amplification

 

Composition of the 2X SYBR Green qPCR Mix

100mM KCl , 5mM MgCl2, 400μM dNTPs, 0.1U/μl Hotstart Taq DNA Polymerase, 1× SYBR® Green I and other optimized buffer components.

 

Table of Instrument Guide

Instruments Final Conc. of ROX
ABI PRISM 7000/ PRISM 7700/ 7300/ 7900HT/ StepOne/ StepOnePlus/ GeneAmp 5700 500 nM,high ROX
ABI 7500/ 7500 Fast/ ViiA 7/ QuantStudio 6/7/12K Flex; Agilent Stratagene Mx3000P/ Mx3005P/ Mx4000 50 nM,low ROX
Bio-Rad CFX96/ CFX384/ iQ/ iQ5; MJ Research Opticon 2/ Chromo 4; Roche LightCycler 480/ 96; Corbett Rotor Gene G/ Q/ 3000/ 6000; Thermo PikoReal 96; Eppendorf MasterCycler ep realplex; Cepheid Smart Cycler No ROX

Real Time SYBR Green QPCR Master Mix With ROX P2091c P2092c 0

Real Time SYBR Green QPCR Master Mix With ROX P2091c P2092c 1

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.

Real Time SYBR Green QPCR Master Mix With ROX P2091c P2092c 2

Contact Details
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Phone Number : +8618898478663