|Place of Origin:||China|
|Minimum Order Quantity:||1Bag|
|Packaging Details:||small package or bulk distribute or OEM|
|Delivery Time:||8 work days|
|Payment Terms:||L/C, D/A, D/P, T/T, Western Union, MoneyGram|
|Supply Ability:||100 Bag/Bags Per Day|
|Cat. No.:||P2091c/P2092c||Specification:||P2091c, 400 Rxn/20μl Reaction P2092c, 2000 Rxn/20μl Reaction|
|Appearance:||Colourless||Group:||Real Time PCR Mix|
|Logo Printing:||With Logo Printing||Transport Package:||Packing|
|Production Capacity:||100 Bag/Bags Per Day||Storage Conditions:||Store At -20°C|
Real Time sybr green qpcr master mix,
sybr green qpcr master mix ROX,
400 rxn sybr green qpcr mix
Cat. No: P2091c, 400 rxn/20μl reaction
P2092c, 2000 rxn/20μl reaction
SYBR® Green qPCR Mix with ROX
SYBR® Green qPCR Mix with ROX P2091c/P2092c
For research use only
|2X SYBR® Green qPCR Mix||1 ml||1 ml × 5|
|50X ROX Reference Dye H*||40 μl||200 μl|
|50X ROX Reference Dye L*||40 μl||200 μl|
|Nuclease-free Water||1 ml||1 ml × 5|
* ROX Reference Dye H and ROX Reference Dye L are suitable for fluorescence quantitative PCR instruments requiring high and low concentrations of ROX calibration respectively. It is recommended that 40 μL 50X ROX Reference Dye H or L be premixed into 1ml 2X SYBR Green qPCR Mix. The final working concentration of ROX is 1X.
This mix can be stored for 2 months at 4°C and protected from light. For longer storage, it should be kept at -20°C and protected from light. The ROX Reference Dye should be kept at -20°C and protected from light.
SYBR® Green qPCR Mix with ROX is designed for high-performance, high-throughput real-time PCR. The kit contains Taq DNA Polymerase engineered through a process of molecular evolution. The result is a unique polymerase that specifically designed for qPCR using SYBR® Green I chemistry dye.
2X SYBR® Green qPCR Mix with ROX is a convenient premix of the components (except primers and DNA template) that necessary to perform real-time polymerase chain reaction (PCR) using SYBR® Green I dye with enhanced sensitivity and specificity. The SYBR® Green I dye binds to double-stranded DNA (dsDNA), thus providing a fluorescent signal that reflects the amount of dsDNA products generated during PCR.
• Gene expression analysis
• Low-copy gene detection
• Microarray validation
• Gene knockdown validation
• Compatible with many Real-time systems whether requires ROX reference dye to calibration or not
• Hot-start technology brings high specificity and reproducible amplification
Composition of the 2X SYBR Green qPCR Mix
100mM KCl , 5mM MgCl2, 400μM dNTPs, 0.1U/μl Hotstart Taq DNA Polymerase, 1× SYBR® Green I and other optimized buffer components.
Table of Instrument Guide
|Instruments||Final Conc. of ROX|
|ABI PRISM 7000/ PRISM 7700/ 7300/ 7900HT/ StepOne/ StepOnePlus/ GeneAmp 5700||500 nM,high ROX|
|ABI 7500/ 7500 Fast/ ViiA 7/ QuantStudio 6/7/12K Flex; Agilent Stratagene Mx3000P/ Mx3005P/ Mx4000||50 nM,low ROX|
|Bio-Rad CFX96/ CFX384/ iQ/ iQ5; MJ Research Opticon 2/ Chromo 4; Roche LightCycler 480/ 96; Corbett Rotor Gene G/ Q/ 3000/ 6000; Thermo PikoReal 96; Eppendorf MasterCycler ep realplex; Cepheid Smart Cycler||No ROX|
Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.