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White Powder DNase I Powder N9069 1g For Research Use

Basic Information
Place of Origin: China
Brand Name: GDSBio
Certification: /
Model Number: N9069
Minimum Order Quantity: 1 bag
Packaging Details: small package or bulk distribute or OEM
Delivery Time: 8work days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 100 Bag/Bags Per Day
Detail Information
Cat. No.: N9069 Size: 1g
Concentration: ≥ 500 U/mg Appearance: White Powder
Group: In Vitro Diagnostic Products Recommend Use Concentration: /
Logo Printing: With Logo Printing Transport Package: Packing
Production Capacity: 100 Bag/Bags Per Day Storage Conditions: Store At -20°C
High Light:

White Powder DNase I Powder

,

1g DNase I Powder

,

1g N9069


Product Description

DNase I Powder

BR Grade, For Research Use Only

Cat. No. N9069

Size: 1 g

Specific Activity: ≥ 500 U/mg

Form: Contains 1 g DNase I, lyophilized powder

Store at -20°C for 2 years.

 

Description

DNase I is an endonuclease that nonspecifically cleaves DNA to release di-, tri- and oligonucleotide products with 5´-phosphorylated and 3´-hydroxylated ends. DNase I acts on single- and double-stranded DNA, chromatin and RNA: DNA hybrids.

 

Applications

• Degradation of DNA template in transcription reactions

• DNase I footprinting

• Nick translation

 

Definition of Activity Unit

One unit of the enzyme completely degrades 1 µg of plasmid DNA in 10 min at 37°C. Enzyme activity is assayed in the following mixture: 40 mM Tris-HCl (pH 8.0), 10 mM MgSO4, 1 mM CaCl2, 1 µg of pUC19 DNA.

 

Source

Bovine pancreas.

 

Molecular Weight

38 kDa monomer.

 

Quality Control

Functionally tested for digestion of template DNA after in vitro transcription.

 

Inhibition and Inactivation

Inhibitors: metal chelators, transition metals (e.g., Zn) in millimolar concentrations, SDS (even at concentrations less than 0.1%), reducing agents (DTT and beta-mercaptoethanol), ionic strength above 50-100 mM.

Inactivated by heating at 65°C for 10 min in the presence of EGTA or EDTA (use at least 1 mol of EGTA/EDTA per 1 mol of Mn2+/Mg2+).

 

Note

• DNase I is sensitive to physical denaturation. Mix gently by inverting the tube. Do not vortex.

• This grade of DNase I is not used for removing DNA from RNA preparations.

 

White Powder DNase I Powder N9069 1g For Research Use 0

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