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GDSBio In Vitro Diagnostic Products Molecular Biology Grade Proteinase K Powder PK N9016 100mg

Basic Information
Place of Origin: China
Brand Name: GDSBio
Certification: /
Model Number: N9016
Minimum Order Quantity: 1 bag
Packaging Details: small package or bulk distribute or OEM
Delivery Time: 8work days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 100 Bag/Bags Per Day
Detail Information
Cat. No.: N9017 Size: 100mg
Concentration: Contains Proteinase K (≥95%) Appearance: White Powder
Group: In Vitro Diagnostic Products Specific Activity: ≥30 U/mg Protein
Logo Printing: With Logo Printing Transport Package: Packing
Production Capacity: 100 Bag/Bags Per Day Storage Conditions: Store At -20°C
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GDSBio In Vitro Diagnostic Products

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Molecular Biology Grade In Vitro Diagnostic Products

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100mg Proteinase K Solution


Product Description

Proteinase K Powder

Molecular Biology Grade,For Research Use Only

Cat No. N9016

Size: 100mg

Specific Activity: ≥30 U/mg protein

Form: Contains proteinase K (≥95%), lyophilized powder

Components

Component N90116

Proteinase K Powder

100mg

 

Storage

-20ºC recommended. The shelf life of proteinase K lyophilized powder is three years when stored sealed and dry below 4ºC. Proteinase K lyophilized powder is shipped at room temperature.

 

Description

Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. The Proteinase K is classified as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide.

 

Features

• Recombinant proteinase K

• Active in a wide range of reaction products

 

Applications

• Isolation of genomic DNA from cultured cells and tissues

• Removal of DNases and RNases when isolating DNA and RNA from tissues or cell lines

• Determination of enzyme localization

• Improving cloning efficiency of PCR products

 

Quality Control

DNase Activity: None detectable enzyme activity after 6 hrs incubation with λ DNA at 37ºC.

RNase Activity: None detectable ribonuclease activity after 16 hrs incubation with RNA at 25ºC.

 

Source

From yeast cells with cloned gene encoding genetically engineered Engyodontium album (Tritirachium album) endolytic protease.

 

Molecular Weight

29.3 kDa monomer.

 

Definition of Activity Unit

One unit of the enzyme liberates Folin-positive amino acids and peptides corresponding to 1 µmol tyrosine in 1 min at 37°C , pH 7.5 using denatured hemoglobin as substrate.

Enzyme activity is assayed in the following mixture: 0.08 M potassium phosphate (pH 7.5), 5 M urea, 4 mM NaCl, 3 mM CaCl2 and 16.7 mg/ml hemoglobin.

 

Preparation Instructions

Stock solution can be prepared as 40-80mg/ml in dilution buffer [20 mM Tris-HCl (pH 7.4), 1 mM CaCl2] or [20 mM Tris-HCl (pH 7.4), 1 mM CaCl2, 2% Glycerol], sterilized using 0.22μm filter and supplied at final concentration of 20-40mg/ml in 50% Glycerol. Store in aliquots at wide temperature range from 24ºC to -80ºC.

PES and PVDF membranes with low protein binding are recommended in sterile filtration.

 

Inhibition and Inactivation

Inhibitors: Proteinase K is not inactivated by metal chelators, by thiol-reactive reagents or by specific trypsin and chymotrypsin inhibitors. Phenylmethylsulfonyl fluoride and diisopropyl phosphorofluoridate completely inhibit the enzyme.

Inactivated by heating at 95°C for 10 minutes.

 

Note

• Optimum activity at 50-55°C.

• Rapid denaturation of enzyme occurs at temperatures above 65°C.

• The recommended working concentration for Proteinase K is 0.05-1 mg/ml. The activity of the enzyme is stimulated by 0.2-1% SDS or by 1-4 M urea.

• Ca2+ protects Proteinase K against autolysis, increases the thermal stability and has a regulatory function for the substrate binding site of Proteinase K.

• Stable over a wide pH range: 4.0-12.5, optimum pH 7.5-8.0.

GDSBio In Vitro Diagnostic Products Molecular Biology Grade Proteinase K Powder PK N9016 100mg 0

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