| Place of Origin: | China |
|---|---|
| Brand Name: | GDSBio |
| Certification: | / |
| Model Number: | P2301/P2302 |
| Minimum Order Quantity: | 1Bag |
| Packaging Details: | small package or bulk distribute or OEM |
| Delivery Time: | 8 work days |
| Payment Terms: | L/C, D/A, D/P, T/T, Western Union, MoneyGram |
| Supply Ability: | 100 Bag/Bags Per Day |
| Cat. No.: | P2301/P2302 | Specification: | P2301, 400 Rxn/20μl Reaction P2302, 2000 Rxn/20μl Reaction |
|---|---|---|---|
| Appearance: | Colourless | Group: | Real Time PCR Mix |
| Activity: | Pass | Amplification Capability: | Good |
| Logo Printing: | With Logo Printing | Transport Package: | Packing |
| Production Capacity: | 100 Bag/Bags Per Day | Storage Conditions: | Store At -20°C |
| High Light: | HS Probe master mix for real time pcr,400 rxn master mix for real time pcr,400 rxn pcr reagent mix |
||
Cat. No: P2301, 400 rxn/20μl reaction
P2302, 2000 rxn/20μl reaction
HS Probe qPCR Mix with UDG
For research use only
Components
| Component | P2301 | P2302 |
| 2× HS Probe qPCR Mix with UDG | 1 ml | 1 ml × 5 |
| Nuclease-free Water | 1 ml | 1 ml × 5 |
Storage
This reagent can be stored for 2 months at 4°C. For longer storage, it should be kept at -20°C.
Description
HS Probe qPCR Mix with UDG is designed for high-performance, high-throughput real-time PCR using sequence-specific fluorogenic probes. It contains the components (except primers, DNA template and probe) that necessary to perform PCR. The core component of the mix is OptimusTM Hotstart Taq DNA Polymerase, a hot-start polymerase with antibody-like modification, which brings high specificity by reducing non-specific products as the polymerase activity is temperature-dependent and inhibited at room temperature. Combined with well-optimized buffer, it can significantly improve amplification efficiency. This reagent introduced dUTP/UDG system, which can remove the PCR products containing dUTP before PCR reaction, and effectively avoid the influence of cross-contamination of amplification products on the quantification. This product is perfectly compatible with common quantitative PCR instruments, such as ABI, Roche, Bio-Rad, etc.
Applications
• Probe gene expression analysis
• Probe Low-copy gene detection
• Probe microarray validation
• Probe gene knockdown validation
Features
• This kit is suitable for fluorescence quantification by probe method
• This kit is compatible with many real-time systems
• Hot-start technology brings high specificity and reproducible amplification
• dUTP/UDG system, effectively prevent PCR product contamination
Composition of the 2× HS Probe qPCR Mix with UDG
KCl, MgCl2, dNTP/dUTP Mix, OptimusTM Hotstart Taq DNA Polymerase, UDG (Uracil-DNA Glycosylase) and other optimized buffer components.
Table of Instrument Guide
| Instrument | Conc. of ROX (100×) |
| ABI® PRISM® 7000, 7700, 7900HT, ABI® 7300 qPCR Systems, GeneAmp® 5700, StepOne™, and the StepOnePlus™ |
1-2% ROX
|
| ABI® 7500 qPCR Systems, ViiA™ 7, QuantStudio™ 12K Flex, Agilent Mx3000P™ Mx3005P™ and Mx4000™ |
0.2% ROX
|
| BioRad iCycler MiniOpticon, Opticon 2, Chromo 4, iQ5; Roche LightCycler 480; MJ Research DNA Engine Opticon 2, Chromo 4; Corbett Rotogene 3000, 6000 |
No ROX
|
Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.
