Fast DNA Library Prep Kit For High-throughput Sequencing Platform

Fast DNA Library Prep Kit For High-throughput Sequencing Platform

Product Details:

Place of Origin: China
Brand Name: GDSBio
Certification: ISO9001, ISO13485
Model Number: K001-A, K001-B

Payment & Shipping Terms:

Minimum Order Quantity: 6 rxns
Packaging Details: Carton Packaging
Delivery Time: 8work days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 500000Kits/Week
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Detail Information

Package Size: K001-A/24 Rxns; K001-B/96 Rxns; Sample Sack/ 6 Rxns Storage: Stored At 5 ~ 25°C, With A Validity Period Of 12 Months
Adapters: Standard, Customized Delivery Format: Plates, Tubes
End Repair: Yes Fragmentation Method: Ultrasonic, Enzymatic
Input Amount: 10ng-1ug Input Type: Plasmid, PCR Product, Genomic DNA, Total RNA
Library Normalization: Yes Library Quality Control: Qubit, Bioanalyzer, QPCR
Library Quantification: Qubit, QPCR Library Type: DNA/RNA
Product Name: NGS Library Construction Service Support: Yes
Size Selection: Yes
High Light:

5ml virus sampling tube inactivated


2ml virus sampling tube inactivated


10ml viral transport medium tube with swab

Product Description

Fast DNA Library Prep Kit

Instruction for Use

【Product Name】

Fast DNA Library Prep Kit

【Cat. No./Spec.】

K001-A/24 rxns; K001-B/96 rxns; sample sack/ 6 rxns

【Product Description】

Aiming at Illumina high-throughput sequencing platform, this kit provides a convenient and universal DNA library construction scheme in one tube. It combines end repair and A-Tailing into one step, and reagents are highly premixed, greatly shortening the time of library construction and reducing the error caused by tedious steps. The end preparation, adapter ligation, amplification and purification of the fragmented double-stranded DNA can be performed within about 2 hours. Complete library quantification can be performed by dsDNA fluorescent dye method (e.g., Thermo Qubit Flex Fluorometer) or absolute quantification PCR after diluting the library to an appropriate concentration.

【Sample Type】

Application Sample Type Recommended Amount
Whole genome sequencing High quality complex genomes 50ng-1μg
Target capture sequencing of whole exome High quality complex genomes 10ng-1μg
Target capture sequencing of whole genome FFPE DNA ≥50ng
Target capture sequencing of whole genome cfDNA/ctDNA ≥100pg
Whole genome sequencing Microbial genome 1ng-1μg
Whole genome sequencing(PCR-free) High quality DNA

≥50ng (no size selection)

≥200ng (size selection)

ChIP-Seq ChIP DNA ≥100pg
Targeted sequencing Amplicon ≥100pg

【Storage Condition & Shelf Life】

All reagents should be stored at -20°C. Ligation Buffer is normal for crystals to precipitate at low temperatures, it should be balanced to room temperature before use. The product is valid for 12 months.


Component 24 rxns 96 rxns
End Repair & A-Tailing Mix 480 μl 4×480 μl
Fast DNA Ligase 120 μl 2×240 μl
Fast Ligation Buffer 600 μl 4×600 μl
2× HIFI Library PCR Master Mix 600 μl 4×600 μl
Primer Mix* 120 μl 480 μl

* If there are more than one sample, #K002 and #K003 adapter primer mix is recommended. This kit provides a set of primers with index.

Note:recommended selection beads: #NC1011 GDSPure DNA Selection Magbeads or AMPure XP beads.


Patented formula, contains highly efficient lysate for rapid lysis and inactivation of virus, and eliminate biosafety hazards
Fast DNA Library Prep Kit For High-throughput Sequencing Platform 0
Fast DNA Library Prep Kit For High-throughput Sequencing Platform 1
Environmentally friendly and strong carton packing

Disposable Specimen Collection Sampling Tube Virus Transport Medium Vtm St04


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