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Nucleic Acid PCR Detection Pfu Master Mix P3022 1ml×5

Basic Information
Place of Origin: China
Brand Name: GDSBio
Certification: /
Model Number: P3022
Minimum Order Quantity: 1Bag
Packaging Details: small package or bulk distribute or OEM
Delivery Time: 8 work days
Payment Terms: L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability: 100 Bag/Bags Per Day
Detail Information
Cat. No.: P3022 Specification: P3022 1ml×5
Appearance: Blue Buffer Group: PCR Master Mix
Activity: Pass Amplification Capability: Good
Logo Printing: With Logo Printing Transport Package: Packing
Production Capacity: 100 Bag/Bags Per Day Storage Conditions: Store At -20°C
High Light:

Nucleic Acid PCR Detection pfu master mix

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pfu master mix 1ml

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1ml 2x pfu mix


Product Description

Pfu Kit

#P3022, 5 ml

Pfu Kit For Nucleic Acid PCR Detection P3022 1ml×5

 

Contents:

2× Pfu Reaction Mix 1 ml × 5
Pfu DNA Polymerase (2.5U/μl) 160 μl

 

Store at -20°C

For research use only.

In total 6 vials.

 

Description

Pfu Kit contains Pfu DNA Polymerase and a premixed, ready-to-use Pfu Reaction Mix, which contains dNTPs, Mg2+ and other components at optimal concentrations for efficient amplification of DNA templates by PCR. To prepare the final PCR, only need to add primers and template DNA. The reaction mix contributes to high specificity by optimizing the system, reducing primer-dimer rate. PCR amplification sensitivity is controllable due to the amount of DNA polymerase is flexible.

Pfu DNA Polymerase, derived from the hyperthermophilic archae Pyrococcus furiosus, has been shown to exhibit superior thermostability and proofreading properties compared to other thermostable polymerase. Unlike Taq DNA Polymerase, highly thermostable Pfu DNA Polymerase possesses 3' to 5' exonuclease proofreading activity that enables the polymerase to correct nucleotide-misincorporation errors. This means that Pfu DNA Polymerase-generated PCR fragments will have fewer errors than Taq-generated PCR inserts. Using Pfu DNA Polymerase in your PCR reactions results in blunt-ended PCR products, which are ideal for cloning into blunt-ended vectors. Pfu DNA Polymerase is superior for techniques that require high-fidelity DNA synthesis. The elongation velocity is 1kb/min (70~75°C)

Unit Definition

One unit is defined as the amount of the enzyme required to catalyze the incorporation of 10 nmole of dNTPs into an acid-insoluble form in 30 minutes at 70°C using hering sperm DNA as substrate.

Storage Buffer

20mM Tris-HCl (pH8.0), 1mM DTT, 0.1mM EDTA, 100mM KCl, 0.5% NP-40, 0.5% TW 20, 50% Glycerol

Composition of the 2× Pfu Reaction Mix

2× Pfu Buffer, 0.4mM dNTPs, 3.2mM MgSO4, 0.02% bromophenol blue.

Pfu Reaction Mix is a proprietary formulation optimized for robust performance in PCR.

Applications

• High fidelity PCR

• Routine PCR with high reproducibility

• Site-directed mutagenesis

• Generation of PCR products for TA cloning

Features

Convenient: only primers and template DNA are added when prepare final PCR

• High yields of PCR products with minimal optimization

Fast: saves time due to reduced number of pipetting steps.

Reproducible: lower contamination and pipetting error risk

Flexible: the amount of polymerase is flexible and controllable.

Nucleic Acid PCR Detection Pfu Master Mix P3022 1ml×5 0

Nucleic Acid PCR Detection Pfu Master Mix P3022 1ml×5 1

 

Dongsheng Biotech Co., Ltd takes PCR technology as its core, and its products focus on common PCR, qPCR, nucleic acid detection and other fields. Adhering to the quality policy of "unremitting pursuit of continuous innovation, leading technology and customer satisfaction", we provide our customers with cost-effective molecular biology products.

Nucleic Acid PCR Detection Pfu Master Mix P3022 1ml×5 2

Nucleic Acid PCR Detection Pfu Master Mix P3022 1ml×5 3

Contact Details
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Phone Number : +8618898478663